Purpose: Microscopic Imaging Module Vision scientists have used optical imaging to assay function at a variety of length scales - from subcellular processes to collections of cell organized into networks. With the advent of new fluorescent probes and imaging technologies, the possibilities of both measuring and manipulating cells and circuit function are more powerful than ever. Two notable breakthroughs in the past few years have revolutionized the ability of vision scientists to manipulate and probe excitable cells in intact tissue. First, the development of two-photon microscopy allows for the stimulation of fluorescent probes deep in tissue with a minimum amount of phototoxicity. Second, ion channels have been engineered to open and close in response to light, allowing for spatially localized stimulation and/or silencing of individual cells with millisecond time resolution. The vision science community at UC Berkeley is unique in that several of its members have been at the forefront of developing and utilizing these and other new optical technologies that are revolutionizing vision research. UC Berkeley is also fortunate in having several advanced microscopy systems on campus. In particular, the Molecular Imaging Center (MIC) in the LSA building has five confocal/2-photon imaging systems, a spinning disk confocal system with a spatial-light-modulator used for optical excitation, and soon-to-be installed PALM microscope for super-resolution imaging, all designated for shared use.

National Institute of Health (NIH)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1)
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University of California Berkeley
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Wang, Tzu-Ming; Holzhausen, Lars C; Kramer, Richard H (2014) Imaging an optogenetic pH sensor reveals that protons mediate lateral inhibition in the retina. Nat Neurosci 17:262-8
Robertson, Danielle M; Alexander, Larry J; Bonanno, Joseph A et al. (2014) Cornea and ocular surface disease: application of cutting-edge optometric research. Optom Vis Sci 91:S3-16
Tochitsky, Ivan; Polosukhina, Aleksandra; Degtyar, Vadim E et al. (2014) Restoring visual function to blind mice with a photoswitch that exploits electrophysiological remodeling of retinal ganglion cells. Neuron 81:800-13
Vlasits, Anna L; Bos, RĂ©mi; Morrie, Ryan D et al. (2014) Visual stimulation switches the polarity of excitatory input to starburst amacrine cells. Neuron 83:1172-84
Piazza, Elise A; Silver, Michael A (2014) Persistent hemispheric differences in the perceptual selection of spatial frequencies. J Cogn Neurosci 26:2021-7
Denison, Rachel N; Vu, An T; Yacoub, Essa et al. (2014) Functional mapping of the magnocellular and parvocellular subdivisions of human LGN. Neuroimage 102 Pt 2:358-69
Lin, Wan-Chen; Davenport, Christopher M; Mourot, Alexandre et al. (2014) Engineering a light-regulated GABAA receptor for optical control of neural inhibition. ACS Chem Biol 9:1414-9
Piazza, Elise A; Sweeny, Timothy D; Wessel, David et al. (2013) Humans use summary statistics to perceive auditory sequences. Psychol Sci 24:1389-97
Chung, Susana T L (2013) The Glenn A. Fry Award Lecture 2012: Plasticity of the visual system following central vision loss. Optom Vis Sci 90:520-9
Bressler, David W; Fortenbaugh, Francesca C; Robertson, Lynn C et al. (2013) Visual spatial attention enhances the amplitude of positive and negative fMRI responses to visual stimulation in an eccentricity-dependent manner. Vision Res 85:104-12

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