Purpose: Microscopic Imaging Module Vision scientists have used optical imaging to assay function at a variety of length scales - from subcellular processes to collections of cell organized into networks. With the advent of new fluorescent probes and imaging technologies, the possibilities of both measuring and manipulating cells and circuit function are more powerful than ever. Two notable breakthroughs in the past few years have revolutionized the ability of vision scientists to manipulate and probe excitable cells in intact tissue. First, the development of two-photon microscopy allows for the stimulation of fluorescent probes deep in tissue with a minimum amount of phototoxicity. Second, ion channels have been engineered to open and close in response to light, allowing for spatially localized stimulation and/or silencing of individual cells with millisecond time resolution. The vision science community at UC Berkeley is unique in that several of its members have been at the forefront of developing and utilizing these and other new optical technologies that are revolutionizing vision research. UC Berkeley is also fortunate in having several advanced microscopy systems on campus. In particular, the Molecular Imaging Center (MIC) in the LSA building has five confocal/2-photon imaging systems, a spinning disk confocal system with a spatial-light-modulator used for optical excitation, and soon-to-be installed PALM microscope for super-resolution imaging, all designated for shared use.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY003176-32
Application #
8694037
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
32
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Berkeley
Department
Type
DUNS #
City
Berkeley
State
CA
Country
United States
Zip Code
94704
McNamara, Nancy A; Ge, Shaokui; Lee, Salena M et al. (2016) Reduced Levels of Tear Lacritin Are Associated With Corneal Neuropathy in Patients With the Ocular Component of Sjögren's Syndrome. Invest Ophthalmol Vis Sci 57:5237-5243
Rosa, Juliana M; Morrie, Ryan D; Baertsch, Hans C et al. (2016) Contributions of Rod and Cone Pathways to Retinal Direction Selectivity Through Development. J Neurosci 36:9683-95
Denison, Rachel N; Sheynin, Jacob; Silver, Michael A (2016) Perceptual suppression of predicted natural images. J Vis 16:6
Vlasits, Anna L; Morrie, Ryan D; Tran-Van-Minh, Alexandra et al. (2016) A Role for Synaptic Input Distribution in a Dendritic Computation of Motion Direction in the Retina. Neuron 89:1317-30
Metlapally, Sangeetha; Tong, Jianliang L; Tahir, Humza J et al. (2016) Potential role for microfluctuations as a temporal directional cue to accommodation. J Vis 16:19
Arroyo, David A; Kirkby, Lowry A; Feller, Marla B (2016) Retinal Waves Modulate an Intraretinal Circuit of Intrinsically Photosensitive Retinal Ganglion Cells. J Neurosci 36:6892-905
Tochitsky, Ivan; Helft, Zachary; Meseguer, Victor et al. (2016) How Azobenzene Photoswitches Restore Visual Responses to the Blind Retina. Neuron 92:100-113
Firl, Alana; Ke, Jiang-Bin; Zhang, Lei et al. (2015) Elucidating the role of AII amacrine cells in glutamatergic retinal waves. J Neurosci 35:1675-86
Tochitsky, Ivan; Kramer, Richard H (2015) Optopharmacological tools for restoring visual function in degenerative retinal diseases. Curr Opin Neurobiol 34:74-8
Kramer, Richard H; Davenport, Christopher M (2015) Lateral Inhibition in the Vertebrate Retina: The Case of the Missing Neurotransmitter. PLoS Biol 13:e1002322

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