Funds are requested by 29 vision scientists to support four research modules that facilitate and enhance interaction among the investigators in the various disciplines represented at the Schepens Eye Research Institute. The modules will extend endeavors of individual research programs by providing opportunities for projects in which investigators do not have expertise, funding, or technical capabilities. The modules are: Morphology, Animal Resource, Laboratory Computer Applications and Flow Cytometry. The Morphology Module will provide light and electron microscopy, confocal microscopy and tissue preparation. The module houses and maintains shared equipment, and provides instruction on use of equipment. The module is available to all members of the Core making morphological techniques available to all. The Animal Resource Module provides expertise and assistance in animal surgical techniques and postoperative care, administration of anesthesia, drugs, and medications. In addition, it will also provide for the daily animal husbandry that is required to maintain our AALAC standards for animal housing, sanitation, and veterinary care. The Laboratory Computer Applications Module will provide support to individual and collaborative efforts by programming and developing software for gathering, processing, and analyzing experimental data by computer systems. The module will also provide assistance to projects involving image processing and analysis. The module interacts actively with the Morphology and Flow Cytometry Modules supporting analysis and export of data. The Flow Cytometry Module is a centralized service providing principal investigators and collaborators access to equipment and technical support for flow cytometry and cell sorting. Available to the investigators are a Cytomation MoFlo Ultra-high speed cell sorter, an EPICS SL analyzer, a BD LSR II analyzer and a CompuCyte Laser Scanning Cytometer. Personnel with specialized training in the respective fields and equipment staff the modules. Each module is under the immediate supervision of an established, experienced, investigator. The module heads constitute the Core Grant Committee, which is responsible to the Co-directors of Research.

Public Health Relevance

The Institute Core grant provides funds for 29 vision scientists to support four research modules at the Schepens Eye Research Institute, Morphology, Animal Resource, Laboratory Computer Applications and Flow Cytometry. The Modules provide individual researchers to have access to expertise and expensive pieces of equipment that are not available in their lab.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY003790-33
Application #
8511635
Study Section
Special Emphasis Panel (ZEY1-VSN (05))
Program Officer
Liberman, Ellen S
Project Start
1997-08-01
Project End
2016-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
33
Fiscal Year
2013
Total Cost
$970,000
Indirect Cost
$470,000
Name
Schepens Eye Research Institute
Department
Type
DUNS #
073826000
City
Boston
State
MA
Country
United States
Zip Code
02114
Karamichos, Dimitrios; Funderburgh, Martha L; Hutcheon, Audrey E K et al. (2014) A role for topographic cues in the organization of collagenous matrix by corneal fibroblasts and stem cells. PLoS One 9:e86260
Karamichos, D; Hutcheon, A E K; Zieske, J D (2014) Reversal of fibrosis by TGF-?3 in a 3D in vitro model. Exp Eye Res 124:31-6
Liu, Yang; Kam, Wendy R; Ding, Juan et al. (2014) One man's poison is another man's meat: using azithromycin-induced phospholipidosis to promote ocular surface health. Toxicology 320:1-5
Alberti, Concetta F; Horowitz, Todd; Bronstad, P Matthew et al. (2014) Visual attention measures predict pedestrian detection in central field loss: a pilot study. PLoS One 9:e89381
Karamichos, D; Hutcheon, A E K; Rich, C B et al. (2014) In vitro model suggests oxidative stress involved in keratoconus disease. Sci Rep 4:4608
Peli, Eli; Satgunam, PremNandhini (2014) Bitemporal hemianopia; its unique binocular complexities and a novel remedy. Ophthalmic Physiol Opt 34:233-42
Stepp, Mary Ann; Zieske, James D; Trinkaus-Randall, Vickery et al. (2014) Wounding the cornea to learn how it heals. Exp Eye Res 121:178-93
Masli, Sharmila; Sheibani, Nader; Cursiefen, Claus et al. (2014) Matricellular protein thrombospondins: influence on ocular angiogenesis, wound healing and immuneregulation. Curr Eye Res 39:759-74
Karamichos, Dimitrios; Rich, Celeste B; Zareian, Ramin et al. (2013) TGF-*3 stimulates stromal matrix assembly by human corneal keratocyte-like cells. Invest Ophthalmol Vis Sci 54:6612-9
Doherty, Amy L; Bowers, Alex R; Luo, Gang et al. (2013) The effect of strabismus on object detection in the ring scotoma of a monocular bioptic telescope. Ophthalmic Physiol Opt 33:550-60

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