Continuing funds are requested by [7 vision scientists holding 10 NEI R01] grants and their personnel to support [three] resource/service cores. In the [Department of Anatomy/Cell Biology and Ophthalmology]. The cores are central units that enable and enhance vision research at Wayne State University and for [one member, at nearby (15 minutes) Oakland University]. Facilities requested are: Imaging/Histopathology (l/H), [Immunology (I)] and Tissue Culture/Molecular (TC/M). The l/H core will provide for confocal laser scanning [(including 3-D confocal microscopy)], light and electron microscopy, immunofluorescence, and image analysis for Western blots and other cell imaging analyses (e.g., viability, phagocytosis, and quantification of plasma membrane potential), as well as training in equipment use. There also is assistance/training in management of digital images, slit lamp photography and poster and publication production. The I core, in Ophthalmology, will provide sophisticated immunological assays and expert consultation to these vision researchers. It will initiate and stimulate innovative research projects that address emerging questions on the immunological basis of many ocular diseases. Among its functions, it will provide for isolation of blood cells for Staining or viable cryopreservation, immunophenotyping, ELISA evaluation of cytokines/chemokines from tissue lysates and culture supernates, data management and storage, and access to the C6 Accuri Flow cytometer and Cellometer instruments and to the Karmanos Flow facility].The Tissue Culture/Molecular facility will assist and train for preparation of specialized media, isolation, purification and characterization of ocular cells for primary culture, subculture and propagation of established cell lines, cryopreserve cells and [for HEK cell culture for AAV vector packaging and purification, DNA cloning, site directed mutagenesis, and DNA transfection]. Assistance and training Is also available for techniques in molecular biology including PCR array and real time RT-PCR, storage of bacteria, vectors and cDNA constructs. Each core is staffed by a well-trained research assistant and directors are NEI-R01 supported. With the PI, this team functions to enhance NEI-supported studies, and facilitate collaborative efforts in vision.

Public Health Relevance

The cores provide central units promoting vision research at Wayne State and [at nearby Oakland University]. They cost effectively enhance research by providing personnel and equipment, often not supported by individual NEI R01 grants, to advance NEI funded research as priority and provide opportunity for collaboration, and attraction of new investigators to vision research.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Program Officer
Liberman, Ellen S
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Wayne State University
Anatomy/Cell Biology
Schools of Medicine
United States
Zip Code
Singh, Pawan Kumar; Guest, John-Michael; Kanwar, Mamta et al. (2017) Zika virus infects cells lining the blood-retinal barrier and causes chorioretinal atrophy in mouse eyes. JCI Insight 2:e92340
Berkowitz, Bruce A; Podolsky, Robert H; Lenning, Jacob et al. (2017) Sodium Iodate Produces a Strain-Dependent Retinal Oxidative Stress Response Measured In Vivo Using QUEST MRI. Invest Ophthalmol Vis Sci 58:3286-3293
Ye, Eun-Ah; Steinle, Jena J (2017) miR-146a suppresses STAT3/VEGF pathways and reduces apoptosis through IL-6 signaling in primary human retinal microvascular endothelial cells in high glucose conditions. Vision Res 139:15-22
Jiang, Youde; Liu, Li; Steinle, Jena J (2017) Compound 49b Regulates ZO-1 and Occludin Levels in Human Retinal Endothelial Cells and in Mouse Retinal Vasculature. Invest Ophthalmol Vis Sci 58:185-189
Suvas, Susmit (2017) Role of Substance P Neuropeptide in Inflammation, Wound Healing, and Tissue Homeostasis. J Immunol 199:1543-1552
Granneman, James G; Kimler, Vickie A; Zhang, Huamei et al. (2017) Lipid droplet biology and evolution illuminated by the characterization of a novel perilipin in teleost fish. Elife 6:
Liu, Li; Jiang, Youde; Curtiss, Elizabeth et al. (2017) TLR4 regulates insulin-resistant proteins to increase apoptosis in the mouse retina. Inflamm Res 66:993-997
Gao, Nan; Liu, Xiaowei; Wu, Jiayin et al. (2017) CXCL10 suppression of hem- and lymph-angiogenesis in inflamed corneas through MMP13. Angiogenesis 20:505-518
Peng, Xudong; Ekanayaka, Sandamali A; McClellan, Sharon A et al. (2017) Characterization of Three Ocular Clinical Isolates of P. aeruginosa: Viability, Biofilm Formation, Adherence, Infectivity, and Effects of Glycyrrhizin. Pathogens 6:
Liu, Lei-Lei; Spix, Nathan J; Zhang, Dao-Qi (2017) NMDA Receptors Contribute to Retrograde Synaptic Transmission from Ganglion Cell Photoreceptors to Dopaminergic Amacrine Cells. Front Cell Neurosci 11:279

Showing the most recent 10 out of 326 publications