Module Use and Impact The Functional Assessment Module is a resource and service Module providing access to state-of-the-art instrumentation and expertise for the assessment of ocular structure and function in living rodents. Prior to the establishment of this Module, most investigators who wished to evaluate retinal structure or function in small animals had very limited options and relied on terminal experiments performed in individual laboratories. The Department of Ophthalmology and Visual Sciences had a small collection of shared instruments available for use and investigators had access to ERG analysis via collaboration with Dr. Heckenlively or Dr. Khan when their time allowed. Within the past several months, the Functional Assessment Module has greatly increased the range of measurements possible through the purchase of the Micron III, SDOCT, pupillometer, and the optokinetic device. The current function of this Module is to provide investigators and members of their group access to the use of this equipment. In the next funding cycle, staff will be added to the Module to provide training on the use of the equipment and assist investigators with retinal imaging (OCT and Micron III) and visual function tests (ERG, pupillometry, optokinetics). Module staff will 1) perform retinal imaging in anesthetized rodents using the Micron III to assess retinal permeability and provide images to investigators for quantitative analysis (support for image analysis is provided by the Morphology and Imaging Module) and to observe fluorescently labeled proteins or cells;2) perform retinal imaging and analysis by SDOCT to provide retinal microstructure details including retinal layer thickness;3) provide assistance with the measurement and analysis of pupillary responses to specific light stimuli to assess retinal function;4) assist with optokinetic analysis, the measurement of visual acuity and contrast sensitivity by recording the optokinetic reflex or tracking response to a rotating visual stimulus displayed on LCD panels;and 5) perform measurement and analysis of photopic and scotopic ERGs and photopigment regeneration kinetics.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY007003-26
Application #
8434326
Study Section
Special Emphasis Panel (ZEY1-VSN (10))
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
26
Fiscal Year
2012
Total Cost
$32,447
Indirect Cost
$11,581
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Eason, Jessica; Williams, Antionette L; Chawla, Bahaar et al. (2017) Differences in neural crest sensitivity to ethanol account for the infrequency of anterior segment defects in the eye compared with craniofacial anomalies in a zebrafish model of fetal alcohol syndrome. Birth Defects Res 109:1212-1227
Smith, Terry J; Kahaly, George J; Ezra, Daniel G et al. (2017) Teprotumumab for Thyroid-Associated Ophthalmopathy. N Engl J Med 376:1748-1761
Pawar, Mercy; Busov, Boris; Chandrasekhar, Aaruran et al. (2017) FAS apoptotic inhibitory molecule 2 is a stress-induced intrinsic neuroprotective factor in the retina. Cell Death Differ 24:1799-1810
Shahi, Pawan K; Liu, Xinling; Aul, Bryce et al. (2017) Abnormal Electroretinogram after Kir7.1 Channel Suppression Suggests Role in Retinal Electrophysiology. Sci Rep 7:10651
Carver, Kyle A; Lin, C M; Bowes Rickman, Catherine et al. (2017) Lack of the P2X7 receptor protects against AMD-like defects and microparticle accumulation in a chronic oxidative stress-induced mouse model of AMD. Biochem Biophys Res Commun 482:81-86
Walsh, Caroline E; Hitchcock, Peter F (2017) Progranulin regulates neurogenesis in the developing vertebrate retina. Dev Neurobiol 77:1114-1129
Beaudoin, Deborah L; Kupershtok, Mania; Demb, Jonathan B (2017) Selective synaptic connections in the retinal pathway for night vision. J Comp Neurol :
Seo, Seungwoon; Chen, Lisheng; Liu, Wenzhong et al. (2017) Foxc1 and Foxc2 in the Neural Crest Are Required for Ocular Anterior Segment Development. Invest Ophthalmol Vis Sci 58:1368-1377
Zhao, Xiwu; Wong, Kwoon Y; Zhang, Dao-Qi (2017) Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina. Sci Rep 7:7920
Zhao, Xiwu; Reifler, Aaron N; Schroeder, Melanie M et al. (2017) Mechanisms creating transient and sustained photoresponses in mammalian retinal ganglion cells. J Gen Physiol 149:335-353

Showing the most recent 10 out of 186 publications