Module Use and Impact A major function of the MBM Module is to provide access to and training for small and large equipment that would be impractical for a single laboratory to acquire or maintain. The Module maintains shared equipment in good-working order, ensures fair access according to Core Center priorities, and assists in the acquisition of new equipment. The Molecular Biology Module also provides a range of services designed to expand the molecular biology capabilities of vision researchers and make their research activities more efficient. These services range from providing assistance with DNA cloning to aiding in the design, execution and analysis of complex experiments. The majority of these services facilitate research in one of two broad categories: gene expression analysis and manipulation of gene expression. The MBM provides technical services that complement and expand upon services provided by larger campus core facilities. It also provides a repository of molecular biology techniques and reagents that can be efficiently shared by a cadre of vision researchers. The University of Michigan Medical School (UMMS) boasts its outstanding Biomedical Research Core Facilities (www.med.umich.edu/brcf/index.htm), providing state-of-the-art genomics, transcriptomics, proteomics, metabolomics, bioinformatics, viral vector, and transgenic animal production services. However, effective utilization of these core technologies involves relative sophistication in experimental design, sample preparation, data set analysis, quantitative RT-PCR (qRT-PCR) validation of mRNA expression, vector design, DNA cloning, plasmid construction, and genotyping assay development. By providing these services, the Molecular Biology Module facilitates the interaction of our investigators with the UMMS facilities. While the economics of scale dictate that duplication of these services would be cost-ineffective, the MBM provides an additional economics of repetition, alleviating the need for each vision research laboratory to adopt the tools necessary to utilize core technologies and interpret the data provided. Dr. Reed provides expertise in analytical and statistical analysis for experimental design, power estimation, bioinformatics, and investigations of complex data sets.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY007003-27
Application #
8511641
Study Section
Special Emphasis Panel (ZEY1-VSN)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
27
Fiscal Year
2013
Total Cost
$95,968
Indirect Cost
$34,252
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Fernando, Roshini; Atkins, Stephen J; Smith, Terry J (2016) Intersection of Chemokine and TSH Receptor Pathways in Human Fibrocytes: Emergence of CXCL-12/CXCR4 Cross Talk Potentially Relevant to Thyroid-Associated Ophthalmopathy. Endocrinology 157:3779-3787
Puro, Donald G; Kohmoto, Ryohsuke; Fujita, Yasushi et al. (2016) Bioelectric impact of pathological angiogenesis on vascular function. Proc Natl Acad Sci U S A 113:9934-9
Sifuentes, Christopher J; Kim, Jung-Woong; Swaroop, Anand et al. (2016) Rapid, Dynamic Activation of Müller Glial Stem Cell Responses in Zebrafish. Invest Ophthalmol Vis Sci 57:5148-5160
Chen, Lisheng; Gage, Philip J (2016) Heterozygous Pitx2 Null Mice Accurately Recapitulate the Ocular Features of Axenfeld-Rieger Syndrome and Congenital Glaucoma. Invest Ophthalmol Vis Sci 57:5023-5030
Clark, Andrea J; Petty, Howard R (2016) WO3/Pt nanoparticles promote light-induced lipid peroxidation and lysosomal instability within tumor cells. Nanotechnology 27:075103
Korot, Edward; Comer, Grant; Steffens, Timothy et al. (2016) Algorithm for the Measure of Vitreous Hyperreflective Foci in Optical Coherence Tomographic Scans of Patients With Diabetic Macular Edema. JAMA Ophthalmol 134:15-20
Carver, Kyle A; Yang, Dongli (2016) N-Acetylcysteine Amide Protects Against Oxidative Stress-Induced Microparticle Release From Human Retinal Pigment Epithelial Cells. Invest Ophthalmol Vis Sci 57:360-71
Wu, Chris Y; Niziol, Leslie M; Musch, David C et al. (2016) Thyroid-Related Orbital Decompression Surgery: A Multivariate Analysis of Risk Factors and Outcomes. Ophthal Plast Reconstr Surg :
Clark, Andrea J; Petty, Howard R (2016) Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep 6:27039
Zhao, Xiwu; Pack, Weston; Khan, Naheed W et al. (2016) Prolonged Inner Retinal Photoreception Depends on the Visual Retinoid Cycle. J Neurosci 36:4209-17

Showing the most recent 10 out of 148 publications