Module Use and Impact A major function of the MBM Module is to provide access to and training for small and large equipment that would be impractical for a single laboratory to acquire or maintain. The Module maintains shared equipment in good-working order, ensures fair access according to Core Center priorities, and assists in the acquisition of new equipment. The Molecular Biology Module also provides a range of services designed to expand the molecular biology capabilities of vision researchers and make their research activities more efficient. These services range from providing assistance with DNA cloning to aiding in the design, execution and analysis of complex experiments. The majority of these services facilitate research in one of two broad categories: gene expression analysis and manipulation of gene expression. The MBM provides technical services that complement and expand upon services provided by larger campus core facilities. It also provides a repository of molecular biology techniques and reagents that can be efficiently shared by a cadre of vision researchers. The University of Michigan Medical School (UMMS) boasts its outstanding Biomedical Research Core Facilities (, providing state-of-the-art genomics, transcriptomics, proteomics, metabolomics, bioinformatics, viral vector, and transgenic animal production services. However, effective utilization of these core technologies involves relative sophistication in experimental design, sample preparation, data set analysis, quantitative RT-PCR (qRT-PCR) validation of mRNA expression, vector design, DNA cloning, plasmid construction, and genotyping assay development. By providing these services, the Molecular Biology Module facilitates the interaction of our investigators with the UMMS facilities. While the economics of scale dictate that duplication of these services would be cost-ineffective, the MBM provides an additional economics of repetition, alleviating the need for each vision research laboratory to adopt the tools necessary to utilize core technologies and interpret the data provided. Dr. Reed provides expertise in analytical and statistical analysis for experimental design, power estimation, bioinformatics, and investigations of complex data sets.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Michigan Ann Arbor
Ann Arbor
United States
Zip Code
Fernando, Roshini; Grisolia, Ana Beatriz Diniz; Lu, Yan et al. (2018) Slit2 Modulates the Inflammatory Phenotype of Orbit-Infiltrating Fibrocytes in Graves' Disease. J Immunol 200:3942-3949
Zhang, Haonan; Xie, Xinyi; Li, Jia et al. (2018) Removal of choroidal vasculature using concurrently applied ultrasound bursts and nanosecond laser pulses. Sci Rep 8:12848
Kiang, Lee; Ross, Bing X; Yao, Jingyu et al. (2018) Vitreous Cytokine Expression and a Murine Model Suggest a Key Role of Microglia in the Inflammatory Response to Retinal Detachment. Invest Ophthalmol Vis Sci 59:3767-3778
Saera-Vila, Alfonso; Louie, Ke'ale W; Sha, Cuilee et al. (2018) Extraocular muscle regeneration in zebrafish requires late signals from Insulin-like growth factors. PLoS One 13:e0192214
Lu, Yan; Atkins, Stephen J; Fernando, Roshini et al. (2018) CD34- Orbital Fibroblasts From Patients With Thyroid-Associated Ophthalmopathy Modulate TNF-? Expression in CD34+ Fibroblasts and Fibrocytes. Invest Ophthalmol Vis Sci 59:2615-2622
Bian, Zong-Mei; Field, Matthew G; Elner, Susan G et al. (2018) Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells. Exp Eye Res 170:29-39
Cao, Xu; Pattnaik, Bikash R; Hughes, Bret A (2018) Mouse retinal pigment epithelial cells exhibit a thiocyanate-selective conductance. Am J Physiol Cell Physiol 315:C457-C473
Chawla, Bahaar; Swain, William; Williams, Antionette L et al. (2018) Retinoic Acid Maintains Function of Neural Crest-Derived Ocular and Craniofacial Structures in Adult Zebrafish. Invest Ophthalmol Vis Sci 59:1924-1935
Shibata, Maho; Ishizaki, Eisuke; Zhang, Ting et al. (2018) Purinergic Vasotoxicity: Role of the Pore/Oxidant/KATP Channel/Ca2+ Pathway in P2X7-Induced Cell Death in Retinal Capillaries. Vision (Basel) 2:
Lundy, Steven K; Nikoopour, Enayat; Karoukis, Athanasios J et al. (2018) T Helper 1 Cellular Immunity Toward Recoverin Is Enhanced in Patients With Active Autoimmune Retinopathy. Front Med (Lausanne) 5:249

Showing the most recent 10 out of 214 publications