A. Research Plan The Small Animal Ocular Imaging Core (SAOIC) comprises a suite of noninvasive imaging services for living mice and rats, including reflectance and fluorescence fundus imaging with a Micron III mouse digital camera system, optical coherence tomography (OCT), adaptive optics (AO) combined with OCT, scanning laser ophthalmoscopy (SLO) and AO-SLO using custom-built optical systems. These techniques allow widefield to cellular-level resolution imaging of the anterior segment, of the fundus vasculature with fluorescence angiography and phase-variance OCT, and of retinal neuronal and RPE cell structure and function. Mice are the most widely used mammalian model for the investigation of fundamental retinal physiology and cell biology, retinal disease and therapeutics. Noninvasive imaging of mice has become increasingly important in eye research, with commercial instruments such as Phoenix Ltd's Micron III widefield fundus camera (available in our facility) being widely employed. UC Davis has outstanding mouse husbandry facilities, and indeed, has one of the NIH-supported Mutant Mouse Regional Resource Centers (described above). The value of the SAOIC is further enhanced by the Molecular Construct and Packaging Core of this grant, which will provide for the production and packaging of DNA vectors for delivery to the mouse eye either by neonatal injection and iontophoresis, or by viral (AAV) packaging and intravitreal injection.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY012576-16
Application #
8884897
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-06-30
Support Year
16
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
DUNS #
City
Davis
State
CA
Country
United States
Zip Code
95618
Cua, Michelle; Wahl, Daniel J; Zhao, Yuan et al. (2016) Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging. Sci Rep 6:32223
Gorczynska, Iwona; Migacz, Justin V; Zawadzki, Robert J et al. (2016) Comparison of amplitude-decorrelation, speckle-variance and phase-variance OCT angiography methods for imaging the human retina and choroid. Biomed Opt Express 7:911-42
Shen, Yunyun; Pfluger, Trisha; Ferreira, Fernando et al. (2016) Diabetic cornea wounds produce significantly weaker electric signals that may contribute to impaired healing. Sci Rep 6:26525
Thomasy, Sara M; Cortes, Dennis E; Hoehn, Alyssa L et al. (2016) In Vivo Imaging of Corneal Endothelial Dystrophy in Boston Terriers: A Spontaneous, Canine Model for Fuchs' Endothelial Corneal Dystrophy. Invest Ophthalmol Vis Sci 57:OCT495-503
Jian, Yifan; Lee, Sujin; Ju, Myeong Jin et al. (2016) Lens-based wavefront sensorless adaptive optics swept source OCT. Sci Rep 6:27620
Thomasy, Sara M; Eaton, J Seth; Timberlake, Matthew J et al. (2016) Species Differences in the Geometry of the Anterior Segment Differentially Affect Anterior Chamber Cell Scoring Systems in Laboratory Animals. J Ocul Pharmacol Ther 32:28-37
Strom, Ann R; Cortés, Dennis E; Rasmussen, Carol A et al. (2016) In vivo evaluation of the cornea and conjunctiva of the normal laboratory beagle using time- and Fourier-domain optical coherence tomography and ultrasound pachymetry. Vet Ophthalmol 19:50-6
Smit-McBride, Zeljka; Moisseiev, Elad; Modjtahedi, Sara P et al. (2016) Comparison of In Vivo Gene Expression Profiling of RPE/Choroid following Intravitreal Injection of Dexamethasone and Triamcinolone Acetonide. J Ophthalmol 2016:9856736
Sebbag, Lionel; Reilly, Christopher M; Eid, Ramzi et al. (2016) Goblet cell density and distribution in cats with clinically and histologically normal conjunctiva. Vet Ophthalmol 19 Suppl 1:38-43
Strom, Ann R; Cortés, Dennis E; Thomasy, Sara M et al. (2016) In vivo ocular imaging of the cornea of the normal female laboratory beagle using confocal microscopy. Vet Ophthalmol 19:63-7

Showing the most recent 10 out of 206 publications