This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules;Gene Delivery/Quantitafive Molecular Biology;Pathology and Imaging;and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1-VSN (05))
Program Officer
Liberman, Ellen S
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University of Wisconsin Madison
Schools of Medicine
United States
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Teixeira, L B C; Zhao, Y; Dubielzig, R R et al. (2015) Ultrastructural abnormalities of the trabecular meshwork extracellular matrix in Cyp1b1-deficient mice. Vet Pathol 52:397-403
Rosenbaum, Erica E; Vasiljevic, Eva; Brehm, Kimberley S et al. (2014) Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster. PLoS Genet 10:e1004349
Teixeira, Leandro B C; Buhr, Kevin A; Bowie, Owen et al. (2014) Quantifying optic nerve axons in a cat glaucoma model by a semi-automated targeted counting method. Mol Vis 20:376-85
Lee, Eun Suk; Rasmussen, Carol A; Filla, Mark S et al. (2014) Prospects for lentiviral vector mediated prostaglandin F synthase gene delivery in monkey eyes in vivo. Curr Eye Res 39:859-70
Shin, Eui Seok; Huang, Qiong; Gurel, Zafer et al. (2014) High glucose alters retinal astrocytes phenotype through increased production of inflammatory cytokines and oxidative stress. PLoS One 9:e103148
Rasmussen, Carol A; Kaufman, Paul L; Kiland, Julie A (2014) Benzalkonium chloride and glaucoma. J Ocul Pharmacol Ther 30:163-9
Fl├╝gel-Koch, Cassandra M; Tektas, Ozan Y; Kaufman, Paul L et al. (2014) Morphological alterations within the peripheral fixation of the iris dilator muscle in eyes with pigmentary glaucoma. Invest Ophthalmol Vis Sci 55:4541-51
Wang, Yun; Wang, Ying; Yang, Qiaona et al. (2014) Neuroprotective effects of C3 exoenzyme in excitotoxic retinopathy. Exp Eye Res 125:128-34
Gagen, Debjani; Faralli, Jennifer A; Filla, Mark S et al. (2014) The role of integrins in the trabecular meshwork. J Ocul Pharmacol Ther 30:110-20
Rosenbaum, Erica E; Vasiljevic, Eva; Cleland, Spencer C et al. (2014) The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival. J Biol Chem 289:32392-409

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