This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules;Gene Delivery/Quantitafive Molecular Biology;Pathology and Imaging;and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY016665-07
Application #
8306688
Study Section
Special Emphasis Panel (ZEY1-VSN (05))
Program Officer
Liberman, Ellen S
Project Start
2005-07-01
Project End
2016-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
7
Fiscal Year
2012
Total Cost
$602,000
Indirect Cost
$202,000
Name
University of Wisconsin Madison
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
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Wang, Yun; Wang, Ying; Yang, Qiaona et al. (2014) Neuroprotective effects of C3 exoenzyme in excitotoxic retinopathy. Exp Eye Res 125:128-34
Gagen, Debjani; Faralli, Jennifer A; Filla, Mark S et al. (2014) The role of integrins in the trabecular meshwork. J Ocul Pharmacol Ther 30:110-20
Rosenbaum, Erica E; Vasiljevic, Eva; Cleland, Spencer C et al. (2014) The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival. J Biol Chem 289:32392-409

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