This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules;Gene Delivery/Quantitafive Molecular Biology;Pathology and Imaging;and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1-VSN (05))
Program Officer
Liberman, Ellen S
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University of Wisconsin Madison
Schools of Medicine
United States
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Tan, Li Xuan; Toops, Kimberly A; Lakkaraju, Aparna (2016) Protective responses to sublytic complement in the retinal pigment epithelium. Proc Natl Acad Sci U S A 113:8789-94
De Moraes, C Gustavo; Liebmann, Jeffrey M; Levin, Leonard A (2016) Detection and measurement of clinically meaningful visual field progression in clinical trials for glaucoma. Prog Retin Eye Res :
Croft, Mary Ann; Heatley, Gregg; McDonald, Jared P et al. (2016) Accommodative movements of the lens/capsule and the strand that extends between the posterior vitreous zonule insertion zone & the lens equator, in relation to the vitreous face and aging. Ophthalmic Physiol Opt 36:21-32
Saghiri, Mohammad Ali; Orangi, Jafar; Asatourian, Armen et al. (2016) Functional role of inorganic trace elements in angiogenesis part III: (Ti, Li, Ce, As, Hg, Va, Nb and Pb). Crit Rev Oncol Hematol 98:290-301
Peotter, Jennifer L; Phillips, Jenny; Tong, Tiegang et al. (2016) Involvement of Tiam1, RhoG and ELMO2/ILK in Rac1-mediated phagocytosis in human trabecular meshwork cells. Exp Cell Res 347:301-11
Souma, Tomokazu; Tompson, Stuart W; Thomson, Benjamin R et al. (2016) Angiopoietin receptor TEK mutations underlie primary congenital glaucoma with variable expressivity. J Clin Invest 126:2575-87
Kuehn, Markus H; Lipsett, Koren A; Menotti-Raymond, Marilyn et al. (2016) A Mutation in LTBP2 Causes Congenital Glaucoma in Domestic Cats (Felis catus). PLoS One 11:e0154412
Portillo, Jose-Andres C; Corcino, Yalitza Lopez; Miao, Yanling et al. (2016) CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy. Diabetes :
Gosling, Allyson A; Kiland, Julie A; Rutkowski, Lauren E et al. (2016) Effects of topical corticosteroid administration on intraocular pressure in normal and glaucomatous cats. Vet Ophthalmol 19 Suppl 1:69-76
Teixeira, Leandro B C; Hoeve, James N Ver; Mayer, Joshua A et al. (2016) Modeling the Chronic Loss of Optic Nerve Axons and the Effects on the Retinal Nerve Fiber Layer Structure in Primary Disorder of Myelin. Invest Ophthalmol Vis Sci 57:4859-4868

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