This P30 Core Grant for Vision Research application from the University of Wisconsin Vision Researchers proposes three modules;Gene Delivery/Quantitafive Molecular Biology;Pathology and Imaging;and Animal Model and Eye Organ Culture. The ability to efficiently deliver genes to cells is a powerful new therapeutic approach in treating ocular disease and is an essential research tool. The Gene delivery module will construct vectors, prepare high titer stocks of these materials for use by researchers, and assist investigators with gene delivery methods. The use of quantitative methods in molecular biology is growing. In particular, the quantitation of gene expression by RT-PCR and the analysis of gene expression changes using array technology. The Gene Delivery/Quantitative Molecular Biology Module will provide dedicated technical expertise to these areas and will enhance the abilities of those researchers currently using these technologies to add them to their research programs. Pathology analysis and sophisticated imaging methods have become increasingly important, as the emphasis on translational research has grown. The Pathology and Imaging Module will provide advanced histology, microscopy and image analysis, and morphometry of tissue sections and cultured cells. The development of new therapies and improving our understanding of visual system function relies on transferring work done in vitro to animal models. The Animal Model and Eye Organ Culture Module will assist investigators in using animal models (primate, cat, rabbit and rodent), experimental glaucoma induction, imaging and ocular physiology. This expertise will aid the efforts of core users in assisting the effects of their infectious agents, drugs, manipulations, and vectors/constructs on ocular function in rodents, cats, rabbits and primates. During the previous funding period a total of 28 qualifying NEI grants were assisted. As of the submission date, 188 peer reviewed papers have involved use of the core.

National Institute of Health (NIH)
National Eye Institute (NEI)
Center Core Grants (P30)
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Special Emphasis Panel (ZEY1-VSN (05))
Program Officer
Liberman, Ellen S
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University of Wisconsin Madison
Schools of Medicine
United States
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Chan, Wesley; Almasieh, Mohammadali; Catrinescu, Maria-Magdalena et al. (2018) Cobalamin-Associated Superoxide Scavenging in Neuronal Cells Is a Potential Mechanism for Vitamin B12-Deprivation Optic Neuropathy. Am J Pathol 188:160-172
Janus, David A; Lieven, Christopher J; Crowe, Megan E et al. (2018) Polyester-based microdisc systems for sustained release of neuroprotective phosphine-borane complexes. Pharm Dev Technol 23:882-889
Ashwinbalaji, Soundararajan; Senthilkumari, Srinivasan; Gowripriya, Chidambaranathan et al. (2018) SB772077B, A New Rho Kinase Inhibitor Enhances Aqueous Humour Outflow Facility in Human Eyes. Sci Rep 8:15472
Lawlor, Mitchell; Danesh-Meyer, Helen; Levin, Leonard A et al. (2018) Glaucoma and the brain: Trans-synaptic degeneration, structural change, and implications for neuroprotection. Surv Ophthalmol 63:296-306
Gurel, Zafer; Sheibani, Nader (2018) O-Linked ?-N-acetylglucosamine (O-GlcNAc) modification: a new pathway to decode pathogenesis of diabetic retinopathy. Clin Sci (Lond) 132:185-198
Tan, Junkai; Fan, Ning; Wang, Ningli et al. (2018) Effects of Lentivirus-Mediated C3 Expression on Trabecular Meshwork Cells and Intraocular Pressure. Invest Ophthalmol Vis Sci 59:4937-4944
Saghiri, Mohammad Ali; Asatourian, Armen; Sheibani, Nader (2018) Angiogenesis and the prevention of alveolar osteitis: a review study. J Korean Assoc Oral Maxillofac Surg 44:93-102
Saghiri, Mohammad Ali; Asatourian, Armen; Sorenson, Christine M et al. (2018) Mice dental pulp and periodontal ligament endothelial cells exhibit different proangiogenic properties. Tissue Cell 50:31-36
Farnoodian, Mitra; Sorenson, Christine M; Sheibani, Nader (2018) PEDF expression affects the oxidative and inflammatory state of choroidal endothelial cells. Am J Physiol Cell Physiol 314:C456-C472
Haruta, Miyoshi; Tan, Li Xuan; Bushey, Daniel B et al. (2018) Environmental and Genetic Factors Regulating Localization of the Plant Plasma Membrane H+-ATPase. Plant Physiol 176:364-377

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