Neurodegenerative disease research is one of UND's few recognized strengths as identified in 3 separate Strategic research reports. This COBRE Phase 111 funding request will support our neurodegenerative disease research group's administrative core, pilot grant program, and our mass spectrometry and imaging cores. Our COBRE group continues to focus on determining mechanisms responsible for the pathogenesis of and identifying possible therapeutic interventions against neurodegenerative disorders. Since our COBRE funding started 9 years ago, we supported 11 project directors, we developed and supported two cores, we supported the hiring of 9 neuroscience faculty, and we supported with pilot grants 16 neuroscience investigators. COBRE investigators received about $11M in extramural grants, published 242 manuscripts, and a new Neuroscience Research Building was built. Our Mass Spectrometry Core Facility is used almost exclusively for small molecule analysis including metabolomics, lipidomics, drug metabolism, molecule structure identification, and small molecule quantification. The equipment housed in our facility includes a new Waters Synapt G2 QTOF with UPLC and Nanomate, an API-3000 triple quadripole with HPLC, a QSTAR with nanoLC, a Polaris Q ion trap with GC, and a Beckman 2-D HPLC. Our Imaging Core Facility provides access to fluorescence, confocal, multiphoton, and electron microscopy instrumentation. The equipment housed in our facility includes two confocal microscopes (Zeiss 510 META LSCM, Olympus FV300 LSCM), a Zeiss ConfoCor2 fluorescence correlation spectroscopy unit, an Olympus FV1000MPE Basic multiphoton/visible confocal microscope system, and two fluorescence microscopes (Nikon TE300 equipped for FURA2 and CFP/YFP FRET ratiometric imaging, and a Nikon Eclipse 801). For electron microscopy, we have a scanning (Hitachi 4700) and a transmission (Hitachi 7500) electron microscope, Reichert ultra-microtomes, and a range of ancillary equipment. The COBRE grant-supported neurodegenerative disease research group has been supported strongly by UND, our successes are recognized by UND, and UND is fully committed to helping sustain our efforts after COBRE funding ends.
(provided by applicant): COBRE Phase III funding will (1) support 2 cores essential to our neuroscience COBRE group as well as other biomedical researchers, (2) support our pilot grant program that enables investigators to generate data necessary for getting their respective grants funded, and (3) support our administrative core that provides for centralized ordering, a visiting speaker program, library acquisitions, and an annual neuroscience symposium. UND is fully committed to ensuring the sustainability of this group after the COBRE grant ends.
|Wang, W; Ye, Y; Li, J et al. (2014) Lyn regulates cytotoxicity in respiratory epithelial cells challenged by cigarette smoke extracts. Curr Mol Med 14:663-72|
|Bhatt, Dhaval P; Rosenberger, Thad A (2014) Acetate treatment increases fatty acid content in LPS-stimulated BV2 microglia. Lipids 49:621-31|
|Smith, Mark D; Bhatt, Dhaval P; Geiger, Jonathan D et al. (2014) Acetate supplementation modulates brain adenosine metabolizing enzymes and adenosine A?A receptor levels in rats subjected to neuroinflammation. J Neuroinflammation 11:99|
|Kawamura Jr, Masahito; Ruskin, David N; Geiger, Jonathan D et al. (2014) Ketogenic diet sensitizes glucose control of hippocampal excitability. J Lipid Res 55:2254-60|
|Brose, Stephen A; Marquardt, Amanda L; Golovko, Mikhail Y (2014) Fatty acid biosynthesis from glutamate and glutamine is specifically induced in neuronal cells under hypoxia. J Neurochem 129:400-12|
|Safratowich, Bryan D; Hossain, Murad; Bianchi, Laura et al. (2014) Amphetamine potentiates the effects of ?-phenylethylamine through activation of an amine-gated chloride channel. J Neurosci 34:4686-91|
|Bae, Mihyun; Patel, Neha; Xu, Haoxing et al. (2014) Activation of TRPML1 clears intraneuronal A? in preclinical models of HIV infection. J Neurosci 34:11485-503|
|Nagamoto-Combs, Kumi; Kulas, Joshua; Combs, Colin K (2014) A novel cell line from spontaneously immortalized murine microglia. J Neurosci Methods 233:187-98|
|Wang, Jiani; Li, Jiangtao; Dasgupta, Shamik et al. (2014) Alterations in membrane phospholipid fatty acids of Gram-positive piezotolerant bacterium Sporosarcina sp. DSK25 in response to growth pressure. Lipids 49:347-56|
|Schott, Micah B; Grove, Bryon (2013) Receptor-mediated Ca2+ and PKC signaling triggers the loss of cortical PKA compartmentalization through the redistribution of gravin. Cell Signal 25:2125-35|
Showing the most recent 10 out of 12 publications