The study of genetic traits in intact animals has been facilitated by transgenic and gene-targeting (gene-disruption/ knockout and gene-duplication) experiments in which desired genetic changes are made in the germ-line. Thus, the creation of genetically engineered transgenic mouse and rat models and gene-targeted mouse strains has provided tremendous advancements in biomedical research. The technology to insert, delete, replace, and/or mutate genomic sequences in the living animal models or in specific tissues of interest has provided enhanced opportunities to reproduce or delete the pathogenesis of human diseases and/or to identify their molecular targets. If the gene of interest is quantitive such as blood pressure and cardiovascular function, it requires great efforts to maintain uniform genetic background. The transgenic and gene-targeting technologies provide novel model systems for undertaking the type of investigation through analysis that have either decreased (gene-knockout) or increased (gene-implication) gene copies at the normal chromosomal location controlled by natural regulatory gene sequences. The primary goal of the Transgenic and Gene-Targeted Animal COBRE Core is to provide each investigator an access to our knowledge, resources, and technical support to establish breeding pairs, genotyping, maintain, and generate new colonies of genetically manipulated mouse and rat colonies. For the Phase 111 period, the Transgenic and Gene-Targeted Animal Core facility will be a major resource that will continue providing genetically established mouse and rat models for hypertension and cardiovascular and renal research to the COBRE investigators and others at the Tulane University Health Sciences Center. The core will provide the service, education, and training to our investigators, research fellows, students, and staff to establish, maintain and generate new breeding pairs, genotyping, and characterization of genetically manipulated mice and rat colonies.
The technology to insert, delete,replace, and mutate genomic sequences in the living animals has provided a great opprotunity to reproduce or delete the pathogenesis of human diseases and to identify their molecular targetsand has contributed greatly to our understanding of the pathophysiology of hypertension and associated diseases. The Transgenic and Gene-Targeted Animal COBRE Core will be critical to provide the state-of-art technology to COBRE investigators and to develop novel approaches of research.
|Drawz, Paul E; Pajewski, Nicholas M; Bates, Jeffrey T et al. (2017) Effect of Intensive Versus Standard Clinic-Based Hypertension Management on Ambulatory Blood Pressure: Results From the SPRINT (Systolic Blood Pressure Intervention Trial) Ambulatory Blood Pressure Study. Hypertension 69:42-50|
|Scialla, Julia J; Asplin, John; Dobre, Mirela et al. (2017) Higher net acid excretion is associated with a lower risk of kidney disease progression in patients with diabetes. Kidney Int 91:204-215|
|Freedman, Barry I; Rocco, Michael V; Bates, Jeffrey T et al. (2017) APOL1 renal-risk variants do not associate with incident cardiovascular disease or mortality in the Systolic Blood Pressure Intervention Trial. Kidney Int Rep 2:713-720|
|Singh, Purnima; Castillo, Alexander; Islam, M Toriqul et al. (2017) Evidence for Prohypertensive, Proinflammatory Effect of Interleukin-10 During Chronic High Salt Intake in the Condition of Elevated Angiotensin II Level. Hypertension 70:839-845|
|Odden, Michelle C; Peralta, Carmen A; Berlowitz, Dan R et al. (2017) Effect of Intensive Blood Pressure Control on Gait Speed and Mobility Limitation in Adults 75 Years or Older: A Randomized Clinical Trial. JAMA Intern Med 177:500-507|
|Bazan, Hernan A; Hatfield, Samuel A; Brug, Aaron et al. (2017) Carotid Plaque Rupture Is Accompanied by an Increase in the Ratio of Serum circR-284 to miR-221 Levels. Circ Cardiovasc Genet 10:|
|Gao, Hong; Molinas, Adrien J R; Miyata, Kayoko et al. (2017) Overactivity of Liver-Related Neurons in the Paraventricular Nucleus of the Hypothalamus: Electrophysiological Findings in db/db Mice. J Neurosci 37:11140-11150|
|Zhan, Min; St Peter, Wendy L; Doerfler, Rebecca M et al. (2017) Patterns of NSAIDs Use and Their Association with Other Analgesic Use in CKD. Clin J Am Soc Nephrol 12:1778-1786|
|Berlowitz, Dan R; Foy, Capri G; Kazis, Lewis E et al. (2017) Effect of Intensive Blood-Pressure Treatment on Patient-Reported Outcomes. N Engl J Med 377:733-744|
|Rodriguez, Carlos J; Still, Carolyn H; Garcia, Katelyn R et al. (2017) Baseline blood pressure control in Hispanics: characteristics of Hispanics in the Systolic Blood Pressure Intervention Trial. J Clin Hypertens (Greenwich) 19:116-125|
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