The study of genetic traits in intact animals has been facilitated by transgenic and gene-targeting (gene-disruption/ knockout and gene-duplication) experiments in which desired genetic changes are made in the germ-line. Thus, the creation of genetically engineered transgenic mouse and rat models and gene-targeted mouse strains has provided tremendous advancements in biomedical research. The technology to insert, delete, replace, and/or mutate genomic sequences in the living animal models or in specific tissues of interest has provided enhanced opportunities to reproduce or delete the pathogenesis of human diseases and/or to identify their molecular targets. If the gene of interest is quantitive such as blood pressure and cardiovascular function, it requires great efforts to maintain uniform genetic background. The transgenic and gene-targeting technologies provide novel model systems for undertaking the type of investigation through analysis that have either decreased (gene-knockout) or increased (gene-implication) gene copies at the normal chromosomal location controlled by natural regulatory gene sequences. The primary goal of the Transgenic and Gene-Targeted Animal COBRE Core is to provide each investigator an access to our knowledge, resources, and technical support to establish breeding pairs, genotyping, maintain, and generate new colonies of genetically manipulated mouse and rat colonies. For the Phase 111 period, the Transgenic and Gene-Targeted Animal Core facility will be a major resource that will continue providing genetically established mouse and rat models for hypertension and cardiovascular and renal research to the COBRE investigators and others at the Tulane University Health Sciences Center. The core will provide the service, education, and training to our investigators, research fellows, students, and staff to establish, maintain and generate new breeding pairs, genotyping, and characterization of genetically manipulated mice and rat colonies.

Public Health Relevance

The technology to insert, delete,replace, and mutate genomic sequences in the living animals has provided a great opprotunity to reproduce or delete the pathogenesis of human diseases and to identify their molecular targetsand has contributed greatly to our understanding of the pathophysiology of hypertension and associated diseases. The Transgenic and Gene-Targeted Animal COBRE Core will be critical to provide the state-of-art technology to COBRE investigators and to develop novel approaches of research.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
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Tulane University
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Liu, Liu; Kashyap, Shreya; Murphy, Brennah et al. (2016) GPER activation ameliorates aortic remodeling induced by salt-sensitive hypertension. Am J Physiol Heart Circ Physiol 310:H953-61
Hsu, Raymond K; Chai, Boyang; Roy, Jason A et al. (2016) Abrupt Decline in Kidney Function Before Initiating Hemodialysis and All-Cause Mortality: The Chronic Renal Insufficiency Cohort (CRIC) Study. Am J Kidney Dis 68:193-202
Pingili, Ajeeth K; Thirunavukkarasu, Shyamala; Kara, Mehmet et al. (2016) 6β-Hydroxytestosterone, a Cytochrome P450 1B1-Testosterone-Metabolite, Mediates Angiotensin II-Induced Renal Dysfunction in Male Mice. Hypertension 67:916-26
Denburg, Michelle R; Hoofnagle, Andrew N; Sayed, Samir et al. (2016) Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes. J Bone Miner Res 31:1128-36
Osis, Gunars; Handlogten, Mary E; Lee, Hyun-Wook et al. (2016) Effect of NBCe1 deletion on renal citrate and 2-oxoglutarate handling. Physiol Rep 4:
Anwar, Imran J; Miyata, Kayoko; Zsombok, Andrea (2016) Brain stem as a target site for the metabolic side effects of olanzapine. J Neurophysiol 115:1389-98
Navar, L Gabriel (2016) 2016 Young Investigator Award of the American Physiological Society Renal Section. Am J Physiol Renal Physiol :ajprenal.00133.2016
Rocco, Michael V; Chapman, Arlene; Chertow, Glenn M et al. (2016) Chronic Kidney Disease Classification in Systolic Blood Pressure Intervention Trial: Comparison Using Modification of Diet in Renal Disease and CKD-Epidemiology Collaboration Definitions. Am J Nephrol 44:130-40
Navaneethan, Sankar D; Roy, Jason; Tao, Kelvin et al. (2016) Prevalence, Predictors, and Outcomes of Pulmonary Hypertension in CKD. J Am Soc Nephrol 27:877-86
Amdur, Richard L; Feldman, Harold I; Gupta, Jayanta et al. (2016) Inflammation and Progression of CKD: The CRIC Study. Clin J Am Soc Nephrol 11:1546-56

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