The mission of the Fluorescence Cytometry (FC) Core Facility is to provide investigators with state-of-the art technology in a cost-effective manner to aid in understanding cellular processes involved in environmental health challenges. Understanding the processes at this level enables researchers to formulate and test theories as to how environmental factors affect health. The Core is home to instruments that use fluorescent markers and/or monoclonal antibodies to investigate cellular processes. The BD FACSAria? is a state-of-the art flow cytometer and high-speed cell sorter. The three-laser, ten filter-set configuration allows the identification of multiple molecular signals to be analyzed simultaneously. Further, the instrument has capabilities of precise sorting of subpopulations of interest for further investigation. The BD FACSCalibur? is a 1-laser, 3-detector flow cytometer that provides basic cell analysis in 5 parameters. The CompuCyte iCys? Laser Scanning Cytometer (LSC) offers similar detection of molecular signals as in flow Cytometry, the difference being that the sample is fixed on a microscope slide or cell culture plates, rather than in a fluid suspension. This allows more types of tissues to be analyzed and permits visualization of tissues and individual cells. The Miltenyi Biotec autoMacs? cell sorter purifies subpopulations by magnetic bead separation for further investigation. The Luminex? 100? analyzes addressable laser bead arrays for capture and detection of multiple analytes in small samples. The Zeiss? Fluorescence Microscope and Imaging System is used to visualize and capture images of fluorescently tagged cells. In addition to providing reliable, well-maintained instrumentation, the Core offers scientific expertise in experiment design and analysis. The complexity of the technology of the instruments, reagents and protocols require a thorough understanding and training. Having a Core Director and Staff Scientist available to oversee projects ensures reliable and reproducible results using accepted methodologies. The combination of available instrumentation and expertise puts the researchers at CEHS as well as other investigators at the University of Montana at a competitive advantage in publishing and obtaining extramural funding.

Public Health Relevance

The Fluorescence Cytometry Core will provide CEHS investigators with accessible and reliable cytometry instrumentation that is state-of-the-art to aid them in their scientific research projects. The Core also serves to provide scientific expertise for training, experiment design and data analysis. Core staff is responsible for maintenance, quality control, instrument update/replacement and sustainability of the Core.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM103338-01A1
Application #
8542075
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C3))
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-03-31
Support Year
1
Fiscal Year
2013
Total Cost
$139,076
Indirect Cost
$40,789
Name
University of Montana
Department
Type
DUNS #
010379790
City
Missoula
State
MT
Country
United States
Zip Code
59812
Larson, Erica L; Vanderpool, Dan; Keeble, Sara et al. (2016) Contrasting Levels of Molecular Evolution on the Mouse X Chromosome. Genetics 203:1841-57
Brown, Traci A; Lee, Joong Won; Holian, Andrij et al. (2016) Alterations in DNA methylation corresponding with lung inflammation and as a biomarker for disease development after MWCNT exposure. Nanotoxicology 10:453-61
Ferguson, Matthew D; Semmens, Erin O; Dumke, Charles et al. (2016) Measured Pulmonary and Systemic Markers of Inflammation and Oxidative Stress Following Wildland Firefighter Simulations. J Occup Environ Med 58:407-13
Jessop, Forrest; Hamilton, Raymond F; Rhoderick, Joseph F et al. (2016) Autophagy deficiency in macrophages enhances NLRP3 inflammasome activity and chronic lung disease following silica exposure. Toxicol Appl Pharmacol 309:101-10
Brown, Traci Ann; Holian, Andrij; Pinkerton, Kent E et al. (2016) Early life exposure to environmental tobacco smoke alters immune response to asbestos via a shift in inflammatory phenotype resulting in increased disease development. Inhal Toxicol 28:349-56
Lee, Joong Won; Jaffar, Zeina; Pinkerton, Kent E et al. (2015) Alterations in DNA methylation and airway hyperreactivity in response to in utero exposure to environmental tobacco smoke. Inhal Toxicol 27:724-30
Montrose, Luke; Noonan, Curtis W; Cho, Yoon Hee et al. (2015) Evaluating the effect of ambient particulate pollution on DNA methylation in Alaskan sled dogs: potential applications for a sentinel model of human health. Sci Total Environ 512-513:489-94
Jessop, Forrest; Holian, Andrij (2015) Extracellular HMGB1 regulates multi-walled carbon nanotube-induced inflammation in vivo. Nanotoxicology 9:365-72
Lacher, Sarah E; Skagen, Kasse; Veit, Joachim et al. (2015) P-Glycoprotein Transport of Neurotoxic Pesticides. J Pharmacol Exp Ther 355:99-107
Cho, Yoon Hee; Woo, Hae Dong; Jang, Yoonhee et al. (2015) The Association of LINE-1 Hypomethylation with Age and Centromere Positive Micronuclei in Human Lymphocytes. PLoS One 10:e0133909

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