The goals for the Multiphoton Microscopy Core Facility are to make advancements on projects that were initiated during the first two phases of the COBRE program and to expand the use of the facility to investigators that require multiphoton microscopy for novel applications, but have limited experience with these techniques. COBRE investigators, in collaboration with other faculty at the Neuroscience Center of Excellence (NCE) have used our multiphoton imaging capabilities to make discoveries in hair cell synapses and plasticity of intrinsic and synaptic properties of dendrites in the hippocampus. The pioneering multiphoton imaging equipment and its applications attracted collaborations that included scientists from outside the Center and University, including Tulane University Medical School. These diverse projects illustrate the applications that can be considered with organized collaboration and core infrastructure. While extending the availability of multiphoton imaging to more researchers in the greater New Orleans area, ultimately the core will attract sufficient new funding, which, in addition to the institutional commitment of the School of Medicine, will make the core self-sufficient by the end of Phase III funding. The three specific aims of this core are: 1) To provide the infrastructure to sustain existing grant projects that listed the core as essential equipment in their NIH applications;2) To utilize the multiphoton facility to implement new research projects;enable collaborations between investigators;create new imaging applications in neuroscience;and help investigators obtain preliminary data for individual grant applications;3) To develop new research strategies by continuing to develop novel capabilities including optogenetics and in vivo imaging.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103340-02
Application #
8536912
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
2
Fiscal Year
2013
Total Cost
$67,393
Indirect Cost
$20,593
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Bazan, Nicolas G (2016) Molecular Principles for Decoding Homeostasis Disruptions in the Retinal Pigment Epithelium: Significance of Lipid Mediators to Retinal Degenerative Diseases. Adv Exp Med Biol 854:385-91
Li, Songhua; Samardzija, Marijana; Yang, Zhihui et al. (2016) Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis. J Neurosci 36:5808-19
Savtchouk, Iaroslav; Sun, Lu; Bender, Crhistian L et al. (2016) Topological Regulation of Synaptic AMPA Receptor Expression by the RNA-Binding Protein CPEB3. Cell Rep 17:86-103
Jin, Minghao; Li, Songhua; Hu, Jane et al. (2016) Functional Rescue of Retinal Degeneration-Associated Mutant RPE65 Proteins. Adv Exp Med Biol 854:525-32
He, Jiucheng; Bazan, Haydee E P (2016) Neuroanatomy and Neurochemistry of Mouse Cornea. Invest Ophthalmol Vis Sci 57:664-74
Musto, Alberto E; Rosencrans, Robert F; Walker, Chelsey P et al. (2016) Dysfunctional epileptic neuronal circuits and dysmorphic dendritic spines are mitigated by platelet-activating factor receptor antagonism. Sci Rep 6:30298
Bhattacharjee, Surjyadipta; Zhao, Yuhai; Dua, Prerna et al. (2016) microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration. PLoS One 11:e0150211
Lee, Minsup; Li, Songhua; Sato, Kota et al. (2016) Interphotoreceptor Retinoid-Binding Protein Mitigates Cellular Oxidative Stress and Mitochondrial Dysfunction Induced by All-trans-Retinal. Invest Ophthalmol Vis Sci 57:1553-62
Calandria, J M; Asatryan, A; Balaszczuk, V et al. (2015) NPD1-mediated stereoselective regulation of BIRC3 expression through cREL is decisive for neural cell survival. Cell Death Differ 22:1363-77
Yang, Hongtian; Hou, Hailong; Pahng, Amanda et al. (2015) Protein Phosphatase-1 Inhibitor-2 Is a Novel Memory Suppressor. J Neurosci 35:15082-7

Showing the most recent 10 out of 56 publications