The goal of this core is to assist investigators with state-of-the-art technologies for 3-D tissue analysis and stereology. This core is the logical progression of the Molecular Neurobiology core, but now allows quantitation and analysis of cellular and molecular markers in tissue in 3-D. The LSUHSC-NCE has made a commitment to continue to capitalize, build, equip, and staff an Imaging Core Facility with state-of-the-art confocal and stereology microscopes and computers that can be used by the NCE and researchers from the surrounding biomedical community. It provides the technology and personnel to assist researchers in the preparation, embedding, sectioning, labeling, and contrast enhancement of tissue samples. Also with this technology, we can create 3-D images by reconstructing objects from optical or physical sections in 3-dimensions, archive this data, and quantitate them with state-of-the art microscopy and stereology software. This core will be of great help to newly hired NCE PI's as they initiate their research programs, and other researchers in different departments at LSUHSC, Tulane, and Xavier who need state-of-the-art imaging and stereology but don't have the resources or experience. This core will enable greater collaborations through imaging interactions, and additional R01, P50, and T32 grant applications. This facility will greatly sustain both the research community at LSUHSC and the greater New Orleans area. Starting in year 4, we will gradually transition to a fee-for-service facility so that by the end of the Sustainability Plan period this core would be fully self sustaining and independent. The three specific aims of this core are to: 1) hire and train a PhD level scientist to run this core and provide a graduate level seminar entitled: """"""""Principles and Practice of Stereology and Confocal Microscopy"""""""";2) enable researchers to implement stereologic quantitation and whole-slide scanning using the new Olympus VS110 microscope and Visiopharm stereology software;and 3) operate the core to actualize COBRE Phase III project goals, and provide assistance to researchers and their projects throughout the process of design and implementation of 3-D confocal imaging.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103340-03
Application #
8668115
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
3
Fiscal Year
2014
Total Cost
$86,525
Indirect Cost
$26,438
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Elison, Jasmine R; Weinstein, Jessica E; Sheets, Kristopher G et al. (2018) Platelet-Activating Factor (PAF) Receptor Antagonism Modulates Inflammatory Signaling in Experimental Uveitis. Curr Eye Res 43:821-827
Alapure, Bhagwat V; Lu, Yan; Peng, Hongying et al. (2018) Surgical Denervation of Specific Cutaneous Nerves Impedes Excisional Wound Healing of Small Animal Ear Pinnae. Mol Neurobiol 55:1236-1243
Dhingra, Anuradha; Bell, Brent A; Peachey, Neal S et al. (2018) Microtubule-Associated Protein 1 Light Chain 3B, (LC3B) Is Necessary to Maintain Lipid-Mediated Homeostasis in the Retinal Pigment Epithelium. Front Cell Neurosci 12:351
Combe, Crescent L; Canavier, Carmen C; Gasparini, Sonia (2018) Intrinsic Mechanisms of Frequency Selectivity in the Proximal Dendrites of CA1 Pyramidal Neurons. J Neurosci 38:8110-8127
Belayev, Ludmila; Hong, Sung-Ha; Menghani, Hemant et al. (2018) Docosanoids Promote Neurogenesis and Angiogenesis, Blood-Brain Barrier Integrity, Penumbra Protection, and Neurobehavioral Recovery After Experimental Ischemic Stroke. Mol Neurobiol 55:7090-7106
Li, Songhua; Sato, Kota; Gordon, William C et al. (2018) Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. J Biol Chem 293:15256-15268
Gao, Jingxia; Hu, Xiao-Dong; Yang, Hongtian et al. (2018) Distinct Roles of Protein Phosphatase 1 Bound on Neurabin and Spinophilin and Its Regulation in AMPA Receptor Trafficking and LTD Induction. Mol Neurobiol 55:7179-7186
Knott, Eric J; Gordon, William C; Jun, Bokkyoo et al. (2018) Retinal Pigment Epithelium and Photoreceptor Preconditioning Protection Requires Docosanoid Signaling. Cell Mol Neurobiol 38:901-917
Bazan, Nicolas G (2018) Docosanoids and elovanoids from omega-3 fatty acids are pro-homeostatic modulators of inflammatory responses, cell damage and neuroprotection. Mol Aspects Med 64:18-33
Gordon, William C; López, Virginia García; Bhattacharjee, Surjyadipta et al. (2018) A Nonsteroidal Novel Formulation Targeting Inflammatory and Pruritus-Related Mediators Modulates Experimental Allergic Contact Dermatitis. Dermatol Ther (Heidelb) 8:111-126

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