The Morphology, Imaging and Instrumentation Core (B) provides the space, facilities, supervision, and training for investigators who use histological methods, and advanced imaging technology to answer research questions. This core builds on research investments from NIH/NCRR Shared Instrumentation Grants, NIH/NCRR COBRE Phase I and II programs, and an NSF Research Infrastructure Improvement Award. Core B provides skilled personnel capable of performing and training users in highly specialized techniques, such as confocal imaging, 3D reconstruction, immunohistochemistry, in situ hybridization, cell sorting, ultrasound, and image analysis;and offers advice with experimental design and analysis of data.
Specific aims are: (a) to maintain the resources for safe and effective use by properly trained research personnel;(b) to assist research projects perform high quality and state-of-the-art image acquisition and data analyses;(c) to help in the design and performance of immunohistochemical and in situ hybridization analyses;(d) to provide a state-of-the-art cell sorting service, and (e) provide programs of outreach and education to investigators using methods and instrumentation available in the core. Faculty and student investigators have access to core facilities to process tissue and operate the histological tools, microscopes, and analysis computers, and are assisted by core staff concerning experimental design, data interpretation and technical information to best utilize facilities and instrumentation. The Core has a 15-year record of providing state-of-the-art service and training to investigators at MUSC and throughout South Carolina. Core staff participates in a weeklong training workshop on confocal microscopy (since 2004), acts as a Beckman Coulter Center of Excellence for development of flow cytometry, and has strong interactions with imaging cores in COBREs at Clemson (Biomaterials) and the University of South Carolina (Colon Cancer).

Public Health Relevance

Cardiovascular diseases are the primary cause of morbidity and mortality in the U.S. This Center of Biomedical Research Excellence conducts research in the mechanisms of normal and abnormal heart development, the developmental basis of adult cardiovascular diseases, and the application of the principles of normal development to guide stem-cell based, tissue regeneration or replacement. The Morphology, Imaging and Instrumentation Core provides advanced imaging and histology support to this effort.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM103342-01
Application #
8471618
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-04-30
Support Year
1
Fiscal Year
2012
Total Cost
$295,002
Indirect Cost
$95,000
Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
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Nolan, Krystal D; Kaur, Jasmine; Isaacs, Jennifer S (2017) Secreted heat shock protein 90 promotes prostate cancer stem cell heterogeneity. Oncotarget 8:19323-19341

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