The Morphology, Imaging and Instrumentation Core (B) provides the space, facilities, supervision, and training for investigators who use histological methods, and advanced imaging technology to answer research questions. This core builds on research investments from NIH/NCRR Shared Instrumentation Grants, NIH/NCRR COBRE Phase I and II programs, and an NSF Research Infrastructure Improvement Award. Core B provides skilled personnel capable of performing and training users in highly specialized techniques, such as confocal imaging, 3D reconstruction, immunohistochemistry, in situ hybridization, cell sorting, ultrasound, and image analysis;and offers advice with experimental design and analysis of data.
Specific aims are: (a) to maintain the resources for safe and effective use by properly trained research personnel;(b) to assist research projects perform high quality and state-of-the-art image acquisition and data analyses;(c) to help in the design and performance of immunohistochemical and in situ hybridization analyses;(d) to provide a state-of-the-art cell sorting service, and (e) provide programs of outreach and education to investigators using methods and instrumentation available in the core. Faculty and student investigators have access to core facilities to process tissue and operate the histological tools, microscopes, and analysis computers, and are assisted by core staff concerning experimental design, data interpretation and technical information to best utilize facilities and instrumentation. The Core has a 15-year record of providing state-of-the-art service and training to investigators at MUSC and throughout South Carolina. Core staff participates in a weeklong training workshop on confocal microscopy (since 2004), acts as a Beckman Coulter Center of Excellence for development of flow cytometry, and has strong interactions with imaging cores in COBREs at Clemson (Biomaterials) and the University of South Carolina (Colon Cancer).

Public Health Relevance

Cardiovascular diseases are the primary cause of morbidity and mortality in the U.S. This Center of Biomedical Research Excellence conducts research in the mechanisms of normal and abnormal heart development, the developmental basis of adult cardiovascular diseases, and the application of the principles of normal development to guide stem-cell based, tissue regeneration or replacement. The Morphology, Imaging and Instrumentation Core provides advanced imaging and histology support to this effort.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM103342-01
Application #
8471618
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
Project End
Budget Start
2012-08-01
Budget End
2013-04-30
Support Year
1
Fiscal Year
2012
Total Cost
$295,002
Indirect Cost
$95,000
Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Richards, Dylan J; Tan, Yu; Coyle, Robert et al. (2016) Nanowires and Electrical Stimulation Synergistically Improve Functions of hiPSC Cardiac Spheroids. Nano Lett 16:4670-8
Fresco, Victor M; Kern, Christine B; Mohammadi, Moosa et al. (2016) Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: EFFECTS ON EMBRYO SURVIVAL. J Biol Chem 291:18730-9
Dupuis, Loren E; Doucette, Lorna; Rice, A Kittrell et al. (2016) Development of myotendinous-like junctions that anchor cardiac valves requires fibromodulin and lumican. Dev Dyn 245:1029-42
Rhett, J Matthew; Wang, Hongjun; Bainbridge, Heather et al. (2016) Connexin-Based Therapeutics and Tissue Engineering Approaches to the Amelioration of Chronic Pancreatitis and Type I Diabetes: Construction and Characterization of a Novel Prevascularized Bioartificial Pancreas. J Diabetes Res 2016:7262680
Mallanna, Sunil K; Cayo, Max A; Twaroski, Kirk et al. (2016) Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes. Stem Cell Reports 7:543-56
Briggs, Laura E; Burns, Tara A; Lockhart, Marie M et al. (2016) Wnt/β-catenin and sonic hedgehog pathways interact in the regulation of the development of the dorsal mesenchymal protrusion. Dev Dyn 245:103-13
Arif, Ehtesham; Sharma, Pankaj; Solanki, Ashish et al. (2016) Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1. Mol Cell Biol 36:1639-54
Grek, Christina L; Rhett, J Matthew; Bruce, Jaclynn S et al. (2016) Connexin 43, breast cancer tumor suppressor: Missed connections? Cancer Lett 374:117-26
Jia, Jia; Coyle, Robert C; Richards, Dylan J et al. (2016) Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications. Acta Biomater 45:110-120
Richards, Dylan; Jia, Jia; Yost, Michael et al. (2016) 3D Bioprinting for Vascularized Tissue Fabrication. Ann Biomed Eng :

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