The overall purpose of the SC Cardiovascular COBRE Gene Function Core Is to provide intellectual and physical resources (specifically transgenic and gene targeted mice) to enhance our understanding of cardiovascular malformations and adult cardiac disease, while supporting all investigators around the state of SC in using genetically modified mice. The renewal proposal of this Core builds upon the previous investments of the NIH/NCRR COBRE Phase I and II programs, NIH/NCRR Shared Instrumentation Grant, NIH/NCRR construction grant, and institutional support. The Gene Function COBRE Core integrates two existing state-of-the-art intramural shared resource facilities each of which are unique within the state of SC: the MUSC Transgenic Facility and the MUSC Gene Targeting Facility. The Gene Function Core also offers plasmid construction, optimizing PCR, genotyping, mentoring PIs, and training lab personnel. Since 2001 the COBRE Gene Function Core has serviced over 50 different investigators and facilitated their research involving >$45 million of extramural grant support. During this time period the Gene Function Core has added new services, like cryopreservation, rederivation, and in vitro fertilization, to facilitate the use and transfer of genetically modified mice. A critical addition has been the ability to make both transgenic and gene targeted mice in the C57BL/6 strain. Since the Gene Function Core provides the only means to make genetically modified mice within the State of South Carolina, this Core will continue to support the research programs of MUSC faculty and throughout the state, as we have over the past 10 years. The major aims of the Gene Function Core during Phase III COBRE funding are: 1) to expand the use of genetically modified mice through consultation and education, 2) to provide the expertise and resources required to design and generate DNA constructs for production of genetically modified mice, 3) to generate new transgenic mice, 4) to generate new gene-targeted mice, 5) to employ business practices and pricing that foster long term sustainability of the core, and 6) to facilitate the mission of the Cardiovascular Developmental Biology Center (CDBC) in understanding the developmental basis of cardiovascular diseases.
The Gene Function Core of the SC Cardiovascular COBRE is the only place in the state of Sout Carolina that provides genetically modified and associated services, along with education and outreach, to facilitate biomedical research throughout the state and region.
|Daoud, Abdelkader; Gopal, Udhayakumar; Kaur, Jasmine et al. (2017) Molecular and functional crosstalk between extracellular Hsp90 and ephrin A1 signaling. Oncotarget 8:106807-106819|
|Beiko, Tatsiana; Janech, Michael G; Alekseyenko, Alexander V et al. (2017) Serum Proteins Associated with Emphysema Progression in Severe Alpha-1 Antitrypsin Deficiency. Chronic Obstr Pulm Dis 4:204-216|
|Ghatak, Shibnath; Markwald, Roger R; Hascall, Vincent C et al. (2017) Transforming growth factor ?1 (TGF?1) regulates CD44V6 expression and activity through extracellular signal-regulated kinase (ERK)-induced EGR1 in pulmonary fibrogenic fibroblasts. J Biol Chem 292:10465-10489|
|Pulkoski-Gross, Michael J; Uys, Joachim D; Orr-Gandy, K Alexa et al. (2017) Novel sphingosine kinase-1 inhibitor, LCL351, reduces immune responses in murine DSS-induced colitis. Prostaglandins Other Lipid Mediat 130:47-56|
|Tan, Yu; Richards, Dylan; Coyle, Robert C et al. (2017) Cell number per spheroid and electrical conductivity of nanowires influence the function of silicon nanowired human cardiac spheroids. Acta Biomater 51:495-504|
|Chen, Wei; Zhang, Yong-Mei; Davies, Christopher (2017) Penicillin-Binding Protein 3 Is Essential for Growth of Pseudomonas aeruginosa. Antimicrob Agents Chemother 61:|
|Richards, Dylan; Jia, Jia; Yost, Michael et al. (2017) 3D Bioprinting for Vascularized Tissue Fabrication. Ann Biomed Eng 45:132-147|
|Ghatak, Shibnath; Hascall, Vincent C; Markwald, Roger R et al. (2017) Transforming growth factor ?1 (TGF?1)-induced CD44V6-NOX4 signaling in pathogenesis of idiopathic pulmonary fibrosis. J Biol Chem 292:10490-10519|
|Richards, Dylan J; Coyle, Robert C; Tan, Yu et al. (2017) Inspiration from heart development: Biomimetic development of functional human cardiac organoids. Biomaterials 142:112-123|
|Nolan, Krystal D; Kaur, Jasmine; Isaacs, Jennifer S (2017) Secreted heat shock protein 90 promotes prostate cancer stem cell heterogeneity. Oncotarget 8:19323-19341|
Showing the most recent 10 out of 61 publications