The overall purpose of the SC Cardiovascular COBRE Gene Function Core Is to provide intellectual and physical resources (specifically transgenic and gene targeted mice) to enhance our understanding of cardiovascular malformations and adult cardiac disease, while supporting all investigators around the state of SC in using genetically modified mice. The renewal proposal of this Core builds upon the previous investments of the NIH/NCRR COBRE Phase I and II programs, NIH/NCRR Shared Instrumentation Grant, NIH/NCRR construction grant, and institutional support. The Gene Function COBRE Core integrates two existing state-of-the-art intramural shared resource facilities each of which are unique within the state of SC: the MUSC Transgenic Facility and the MUSC Gene Targeting Facility. The Gene Function Core also offers plasmid construction, optimizing PCR, genotyping, mentoring PIs, and training lab personnel. Since 2001 the COBRE Gene Function Core has serviced over 50 different investigators and facilitated their research involving >$45 million of extramural grant support. During this time period the Gene Function Core has added new services, like cryopreservation, rederivation, and in vitro fertilization, to facilitate the use and transfer of genetically modified mice. A critical addition has been the ability to make both transgenic and gene targeted mice in the C57BL/6 strain. Since the Gene Function Core provides the only means to make genetically modified mice within the State of South Carolina, this Core will continue to support the research programs of MUSC faculty and throughout the state, as we have over the past 10 years. The major aims of the Gene Function Core during Phase III COBRE funding are: 1) to expand the use of genetically modified mice through consultation and education, 2) to provide the expertise and resources required to design and generate DNA constructs for production of genetically modified mice, 3) to generate new transgenic mice, 4) to generate new gene-targeted mice, 5) to employ business practices and pricing that foster long term sustainability of the core, and 6) to facilitate the mission of the Cardiovascular Developmental Biology Center (CDBC) in understanding the developmental basis of cardiovascular diseases.

Public Health Relevance

The Gene Function Core of the SC Cardiovascular COBRE is the only place in the state of Sout Carolina that provides genetically modified and associated services, along with education and outreach, to facilitate biomedical research throughout the state and region.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Medical University of South Carolina
United States
Zip Code
Richards, Dylan J; Tan, Yu; Coyle, Robert et al. (2016) Nanowires and Electrical Stimulation Synergistically Improve Functions of hiPSC Cardiac Spheroids. Nano Lett 16:4670-8
Fresco, Victor M; Kern, Christine B; Mohammadi, Moosa et al. (2016) Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: EFFECTS ON EMBRYO SURVIVAL. J Biol Chem 291:18730-9
Dupuis, Loren E; Doucette, Lorna; Rice, A Kittrell et al. (2016) Development of myotendinous-like junctions that anchor cardiac valves requires fibromodulin and lumican. Dev Dyn 245:1029-42
Rhett, J Matthew; Wang, Hongjun; Bainbridge, Heather et al. (2016) Connexin-Based Therapeutics and Tissue Engineering Approaches to the Amelioration of Chronic Pancreatitis and Type I Diabetes: Construction and Characterization of a Novel Prevascularized Bioartificial Pancreas. J Diabetes Res 2016:7262680
Mallanna, Sunil K; Cayo, Max A; Twaroski, Kirk et al. (2016) Mapping the Cell-Surface N-Glycoproteome of Human Hepatocytes Reveals Markers for Selecting a Homogeneous Population of iPSC-Derived Hepatocytes. Stem Cell Reports 7:543-56
Briggs, Laura E; Burns, Tara A; Lockhart, Marie M et al. (2016) Wnt/β-catenin and sonic hedgehog pathways interact in the regulation of the development of the dorsal mesenchymal protrusion. Dev Dyn 245:103-13
Arif, Ehtesham; Sharma, Pankaj; Solanki, Ashish et al. (2016) Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1. Mol Cell Biol 36:1639-54
Grek, Christina L; Rhett, J Matthew; Bruce, Jaclynn S et al. (2016) Connexin 43, breast cancer tumor suppressor: Missed connections? Cancer Lett 374:117-26
Jia, Jia; Coyle, Robert C; Richards, Dylan J et al. (2016) Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications. Acta Biomater 45:110-120
Richards, Dylan; Jia, Jia; Yost, Michael et al. (2016) 3D Bioprinting for Vascularized Tissue Fabrication. Ann Biomed Eng :

Showing the most recent 10 out of 47 publications