The Morphology, Imaging and Instrumentation Core (B) provides the space, facilities, supervision, and training for investigators who use histological methods, and advanced imaging technology to answer research questions. This core builds on research investments from NIH/NCRR Shared Instrumentation Grants, NIH/NCRR COBRE Phase I and II programs, and an NSF Research Infrastructure Improvement Award. Core B provides skilled personnel capable of performing and training users in highly specialized techniques, such as confocal imaging, 3D reconstruction, immunohistochemistry, in situ hybridization, cell sorting, ultrasound, and image analysis;and offers advice with experimental design and analysis of data.
Specific aims are: (a) to maintain the resources for safe and effective use by properly trained research personnel;(b) to assist research projects perform high quality and state-of-the-art image acquisition and data analyses;(c) to help in the design and performance of immunohistochemical and in situ hybridization analyses;(d) to provide a state-of-the-art cell sorting service, and (e) provide programs of outreach and education to investigators using methods and instrumentation available in the core. Faculty and student investigators have access to core facilities to process tissue and operate the histological tools, microscopes, and analysis computers, and are assisted by core staff concerning experimental design, data interpretation and technical information to best utilize facilities and instrumentation. The Core has a 15-year record of providing state-of-the-art service and training to investigators at MUSC and throughout South Carolina. Core staff participates in a weeklong training workshop on confocal microscopy (since 2004), acts as a Beckman Coulter Center of Excellence for development of flow cytometry, and has strong interactions with imaging cores in COBREs at Clemson (Biomaterials) and the University of South Carolina (Colon Cancer).

Public Health Relevance

Cardiovascular diseases are the primary cause of morbidity and mortality in the U.S. This Center of Biomedical Research Excellence conducts research in the mechanisms of normal and abnormal heart development, the developmental basis of adult cardiovascular diseases, and the application of the principles of normal development to guide stem-cell based, tissue regeneration or replacement. The Morphology, Imaging and Instrumentation Core provides advanced imaging and histology support to this effort.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
5P30GM103342-02
Application #
8517764
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
2
Fiscal Year
2013
Total Cost
$284,676
Indirect Cost
$91,675
Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Nagalakshmi, Vidya K; Lindner, Volkhard; Wessels, Andy et al. (2015) microRNA-dependent temporal gene expression in the ureteric bud epithelium during mammalian kidney development. Dev Dyn 244:444-56
Lockhart, Marie M; Boukens, Bastiaan J D; Phelps, Aimee L et al. (2014) Alk3 mediated Bmp signaling controls the contribution of epicardially derived cells to the tissues of the atrioventricular junction. Dev Biol 396:8-18
Twal, Waleed O; Klatt, Sandra C; Harikrishnan, Keerthi et al. (2014) Cellularized microcarriers as adhesive building blocks for fabrication of tubular tissue constructs. Ann Biomed Eng 42:1470-81
Duval, D; Lardeux, A; Le Tourneau, T et al. (2014) Valvular dystrophy associated filamin A mutations reveal a new role of its first repeats in small-GTPase regulation. Biochim Biophys Acta 1843:234-44
Ghatak, Shibnath; Misra, Suniti; Norris, Russell A et al. (2014) Periostin induces intracellular cross-talk between kinases and hyaluronan in atrioventricular valvulogenesis. J Biol Chem 289:8545-61
Tan, Yu; Richards, Dylan J; Trusk, Thomas C et al. (2014) 3D printing facilitated scaffold-free tissue unit fabrication. Biofabrication 6:024111
Lockhart, Marie M; Phelps, Aimee L; van den Hoff, Maurice J B et al. (2014) The Epicardium and the Development of the Atrioventricular Junction in the Murine Heart. J Dev Biol 2:1-17
Zhang, Yong-Mei; Noto, Jennifer M; Hammond, Charles E et al. (2014) Helicobacter pylori-induced posttranscriptional regulation of H-K-ATPase ?-subunit gene expression by miRNA. Am J Physiol Gastrointest Liver Physiol 306:G606-13
Jia, Jia; Richards, Dylan J; Pollard, Samuel et al. (2014) Engineering alginate as bioink for bioprinting. Acta Biomater 10:4323-31
Czajka, Caitlin A; Mehesz, Agnes Nagy; Trusk, Thomas C et al. (2014) Scaffold-free tissue engineering: organization of the tissue cytoskeleton and its effects on tissue shape. Ann Biomed Eng 42:1049-61

Showing the most recent 10 out of 12 publications