Towards advancing Center and Institute investigations (and continued Center development), our Progenitor Cell Analysis Core provides expert services, training, instrumentation and resources in three related areas: i) analytical flow cytometry, together with FACS and MACS cell isolation services;ii) high through-put colony/cell imaging, fluorimetry, and luminometry;iii) services/resources for stem &progenitor cell culture, including unique cell populations, plus ES cells and approved cell lines. Several core supported services (eg, flow cytometry and FACS) are indispensable, unique components, and are used widely by Center and Institute investigators. Combined instrumentation as a BD FacsCalibur flow cytometer plus FACSAria-2 cell sorter (near-UV laser) enable complex four color multi-parameter analyses. This includes cell surface markers, as well as (co-) analyses of cell cycle parameters, apoptosis, phosphoprotein signaling, ROS, calcium flux and a number of additional informative cell properties. Our expert staff are also skilled in the enrichment and isolation of rare cell sub-populations (eg, using combined MACS plus FACS approaches). In addition, this core supports basic analyses (and manipulations of) progenitor cell populations (Vicell system, injectable multi-well luminometer, fluorimeter, electroporation/transfection units). Recently, we also have launched several innovative cell-based analyses, with cell and colony scanning via STEMvision as one example. With regards to services and resources for the culture and manipulation of stem and progenitor cells, these benefit further from a dedicated tissue culture facility (Zeiss and Evos fluorescent microscopy systems, cell culture hoods and incubators, hypoxia chambers, clinical centrifuges, specialty reagents). Our Progenitor Cell Analysis Core also interacts directly with Center and Institute cores in Histopathology, Molecular Phenotyping, Confocal Microscopy and Viral Vectors;facilitates productive multi-unit interactions;provides vital roles in new Center and Institute faculty recruitment;and continues to advance our biomedical research investigations (together with nationally competitive grant applications).

Public Health Relevance

The majority of Center and Institute investigators utilize (and often depend critically upon) key services and training as supported by our Progenitor Cell Analysis Core for scientific advancements. In addition, this Core spring-boards innovative approaches (eg, STEMvision, enabling of our new Physiology Core);facilitates productive multi-unit interactions;serves vital roles in new Center and Institute faculty recruitment;and bolsters the advancement of biomedical research plus competitive grant applications.

National Institute of Health (NIH)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Maine Medical Center
United States
Zip Code
Ufkin, Melanie L; Peterson, Sarah; Yang, Xuehui et al. (2014) miR-125a regulates cell cycle, proliferation, and apoptosis by targeting the ErbB pathway in acute myeloid leukemia. Leuk Res 38:402-10
Guay, Justin A; Wojchowski, Don M; Fang, Jing et al. (2014) Death associated protein kinase 2 is expressed in cortical interstitial cells of the mouse kidney. BMC Res Notes 7:345
Favreau, Amanda J; Vary, Calvin P H; Brooks, Peter C et al. (2014) Cryptic collagen IV promotes cell migration and adhesion in myeloid leukemia. Cancer Med 3:265-72
Han, Xiang Hua; Jin, Yong-Ri; Tan, Leonard et al. (2014) Regulation of the follistatin gene by RSPO-LGR4 signaling via activation of the WNT/*-catenin pathway in skeletal myogenesis. Mol Cell Biol 34:752-64
Peterson, Sarah M; Thompson, Jeffrey A; Ufkin, Melanie L et al. (2014) Common features of microRNA target prediction tools. Front Genet 5:23
Verma, Rakesh; Su, Su; McCrann, Donald J et al. (2014) RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast development. J Exp Med 211:1715-22
Fetting, Jennifer L; Guay, Justin A; Karolak, Michele J et al. (2014) FOXD1 promotes nephron progenitor differentiation by repressing decorin in the embryonic kidney. Development 141:17-27
Guntur, Anyonya R; Le, Phuong T; Farber, Charles R et al. (2014) Bioenergetics during calvarial osteoblast differentiation reflect strain differences in bone mass. Endocrinology 155:1589-95
Li, Lei; Byrne, Susan M; Rainville, Nicole et al. (2014) Brief report: serpin Spi2A as a novel modulator of hematopoietic progenitor cell formation. Stem Cells 32:2550-6