Today, the advance in translational research requires complex integration of molecular, cellular, animal and human models. This multitude of approaches exceeds the possibilities of a 'standard'laboratory and demands a specialized environment to enhance the success rate and increase the competiveness of our investigators. The Cell and Molecular Analysis Core was developed starting in the second year ofthe Phase I COBRE program. Today this core offers our researchers access to a unique facility on the LSUHSC campus, which provides the latest state-of the-art methodological approaches in cell and molecular biology. This core provides skilled personnel to assist with and/or perform highly specialized experimental procedures. This core is coordinated by Drs. Catalin Filipeanu and Arthur Haas and is organized in the following units: Proteomics, Mass Spectrometry, Electron Paramagnetic Resonance (EPR) Imaging, HPLC, Cell Culture, and Flow-cytometry.
The Specific Aims of this core are: 1) to provide skilled personnel to assist with and/or perform highly specialized procedures for HPLC separation of proteins, 2D separation of proteins for proteomic analysis by mass spectrometry, cell culture, and EPR imaging, 2) to maintain the resources for safe and effective use 3) to provide programs of education and training ofthe methods and instrumentation available in the Core, and 4) to assist investigators in the generation of project data. These facilities are open to all COBRE investigators, as well as researchers throughout the LSUHSC and other institutions. The availability of multiple types of advanced equipment, sophisticated data analysis systems, and specialized personnel dramatically increase the research achievements by current and previous COBRE and LSUHSC investigators. The COBRE Phase III transition for this Core builds upon the previous investments that our University committed to the NCRR Phase I and Phase II programs. In Phase II, the services provided by this COBRE Cell and Molecular Analysis core were integrated under the umbrella ofthe LSUHSC Proteomics Facility, thus providing the foundation for transformation of this successful core to an Institutional facility.
Cardiovascular diseases remain the number one cause of morbidity and mortality in the United States (1) and in Louisiana one in four deaths are caused by cardiovascular complications (2). This core capitalizes on using the expertise of Core Directors and state-of-the-art instrumentation and methodologies that have been developed over the past 10 years to examine the cellular and molecular basis of cardiovascular disease.
|Delgado-Peraza, Francheska; Ahn, Kwang H; Nogueras-Ortiz, Carlos et al. (2016) Mechanisms of Biased Î²-Arrestin-Mediated Signaling Downstream from the Cannabinoid 1 Receptor. Mol Pharmacol 89:618-29|
|Sanchez, Maria Dulfary; Ochoa, Augusto C; Foster, Timothy P (2016) Development and evaluation of a host-targeted antiviral that abrogates herpes simplex virus replication through modulation of arginine-associated metabolic pathways. Antiviral Res 132:13-25|
|Ibba, Salome' V; Ghonim, Mohamed A; Pyakurel, Kusma et al. (2016) Potential of Inducible Nitric Oxide Synthase as a Therapeutic Target for Allergen-Induced Airway Hyperresponsiveness: A Critical Connection to Nitric Oxide Levels and PARP Activity. Mediators Inflamm 2016:1984703|
|Chuang, Gin C; Xia, Huijing; Mahne, Sarah E et al. (2016) Environmentally Persistent Free Radicals Cause Apoptosis in HL-1 Cardiomyocytes. Cardiovasc Toxicol :|
|Burn, Brendan R; Varner, Kurt J (2015) Environmentally persistent free radicals compromise left ventricular function during ischemia/reperfusion injury. Am J Physiol Heart Circ Physiol 308:H998-H1006|
|Ghonim, Mohamed A; Pyakurel, Kusma; Ibba, Salome V et al. (2015) PARP is activated in human asthma and its inhibition by olaparib blocks house dust mite-induced disease in mice. Clin Sci (Lond) 129:951-62|
|Harrison-Bernard, Lisa M (2015) Sphingolipids, new kids on the block, promoting glomerular fibrosis in the diabetic kidney. Am J Physiol Renal Physiol 309:F685-6|
|Xia, Huijing; de Queiroz, Thyago Moreira; Sriramula, Srinivas et al. (2015) Brain ACE2 overexpression reduces DOCA-salt hypertension independently of endoplasmic reticulum stress. Am J Physiol Regul Integr Comp Physiol 308:R370-8|
|Sriramula, Srinivas; Xia, Huijing; Xu, Ping et al. (2015) Brain-targeted angiotensin-converting enzyme 2 overexpression attenuates neurogenic hypertension by inhibiting cyclooxygenase-mediated inflammation. Hypertension 65:577-86|
|Mendoza, Alberto; Lazartigues, Eric (2015) The compensatory renin-angiotensin system in the central regulation of arterial pressure: new avenues and new challenges. Ther Adv Cardiovasc Dis 9:201-8|
Showing the most recent 10 out of 12 publications