Cell and molecular imaging of tissue/cell preparations stained with histochemical and immunohistochemical markers allows researchers to identify the biochemical components and pathways of specimens at subcellular resolution. The COBRE Imaging and Histology Core has been instrumental in this area and provides investigators with space, facilities, instrumentation, supervision, and training for microscopy of livecells and histological specimens (fixed and frozen), laser capture microdissection, confocal imaging, and a variety of histochemical techniques to investigate cardiovascular biology at the cellular and molecular level.
The specific aims of this Core are: 1)To provide skilled personnel to assist and/or perform highly specialized microscopic techniques including deconvolution, confocal microscopy, 3D reconstruction, co-localization, ratio imaging. Total Internal Reflection Fluorescence (TIRF), fluorescent resonance energy transfer (FRET), and fluorescence recovery after photobleaching (FRAP) of both fixed and live cell samples;2) To assist in the collection, processing and staining of histological samples, including standard and special stains, and immunohistochemical antibody labeling;3) To conduct experiments and assist investigators with the selection of appropriate special stains as well as interpretation of histological and immunohistochemical data; 4) To mentor young investigators and students, and advise senior investigators, on the potential application of microscopic techniques, histological analysis and immunohistochemistry to enhance their research projects and provide avenues towards developing translational research projects;5) To guide image analysis methodologies for histological examination. This Core continues to provide COBRE and University investigators, including faculty, postdoctoral fellows, and graduate students, with imaging recourses and technical assistance. Whether investigators are studying cardiovascular biology at the level ofthe cell, tissue, or whole animal, this core has been instrumental to each of our COBRE researchers in examining the structural and/or histological basis for, or confirmation of, different cardiovascular disease states.

Public Health Relevance

Cardiovascular disease (CVD) is the leading cause of death and a major cause of disability woridwide (1,2). The state-of-the-art technology and experienced personnel provided by this Core will continue to enhance the COBRE investigator's progress towards acquisition of extramural funding and will support the overall mission ofthe Cardiovascular Center of Excellence.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM106392-01A1
Application #
8735441
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C3))
Project Start
Project End
Budget Start
2014-09-09
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
$189,800
Indirect Cost
$59,800
Name
Louisiana State Univ Hsc New Orleans
Department
Type
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Trivedi, Rishi K; Polhemus, David J; Li, Zhen et al. (2018) Combined Angiotensin Receptor-Neprilysin Inhibitors Improve Cardiac and Vascular Function Via Increased NO Bioavailability in Heart Failure. J Am Heart Assoc 7:
Tsumagari, Koji; Abd Elmageed, Zakaria Y; Sholl, Andrew B et al. (2018) Bortezomib sensitizes thyroid cancer to BRAF inhibitor in vitro and in vivo. Endocr Relat Cancer 25:99-109
Ghonim, Mohamed A; Wang, Jeffrey; Ibba, Salome V et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway. J Transl Med 16:243
Connick, J Patrick; Reed, James R; Backes, Wayne L (2018) Characterization of Interactions Among CYP1A2, CYP2B4, and NADPH-cytochrome P450 Reductase: Identification of Specific Protein Complexes. Drug Metab Dispos 46:197-203
Lassak, Adam; Dean, Mathew; Wyczechowska, Dorota et al. (2018) Molecular and Structural Traits of Insulin Receptor Substrate 1/LC3 Nuclear Structures and Their Role in Autophagy Control and Tumor Cell Survival. Mol Cell Biol 38:
Carvalho-Galv√£o, Alynne; Ogunlade, Blessing; Xu, Jiaxi et al. (2018) Central administration of TRV027 improves baroreflex sensitivity and vascular reactivity in spontaneously hypertensive rats. Clin Sci (Lond) 132:1513-1527
Berger, Nathan A; Besson, Valerie C; Boulares, A Hamid et al. (2018) Opportunities for the repurposing of PARP inhibitors for the therapy of non-oncological diseases. Br J Pharmacol 175:192-222
Kharbanda, Kusum K; Ronis, Martin J J; Shearn, Colin T et al. (2018) Role of Nutrition in Alcoholic Liver Disease: Summary of the Symposium at the ESBRA 2017 Congress. Biomolecules 8:
Xu, Jiaxi; Sriramula, Srinivas; Lazartigues, Eric (2018) Excessive Glutamate Stimulation Impairs ACE2 Activity Through ADAM17-Mediated Shedding in Cultured Cortical Neurons. Cell Mol Neurobiol :
Flanagan, M; Li, C; Dietrich, M A et al. (2018) Downregulation of heat shock protein B8 decreases osteogenic differentiation potential of dental pulp stem cells during in vitro proliferation. Cell Prolif 51:e12420

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