The GeneLab Core Facility provides molecular biology support, reagents and services to all Centerfor Experimental Infectious Disease Research (CEIDR) investigators. GeneLab sen/ices include general support and state-of-the art next generation sequencing (NGS) and bioinformatics support services. Of primary importance to all CEIDR faculty at Louisiana State University (LSU) and the Tulane National Primate Research Center (TNPRC) is the relatively new (one year) GeneLab state-of-the-art services, which are devoted to NGS and bioinformatics. These activities were started after consensus decisions were reached among CEIDR researchers that they were critically needed by ongoing NIH-funded research projects, as well as the entire Louisiana-based NIH-funded community. NGS equipment includes a Life Technologies, Inc. equipment suite with an Ion PGM and Ion Proton Sequencing Machines, as well as a Quant Studio Flex system equipped with digital PCR capabilities. GeneLab NGS activities are focused on confirmation and sequencing of viral and bacterial genomes, as well as the determination of pathogen and host transcriptional changes during host-pathogen interactions. Bioinformatics support services are provided through a collaboration with the Center for Computation and Technology (CCT), and the Louisiana Biomedical Research Network (LBRN), which is supported by the NIH: NIGMS INBRE grant P20GM103424. GeneLab is currently staffed with 5 PhD-level scientists, one research associate, two computer science graduate students and two undergraduate student workers. In addition, James Cavalcoli, PhD, Director of the Bionformatics Core at the University of Michigan, Ann Arbor serves as a consultant providing expert advice and support, as well as a resource for state-of-the art bioinformatics knowledge. The GeneLab Core fulfills a highly unmet need in the State of Louisiana, since there is no other similar support Core laboratory in the state.GeneLab's efforts in NGS and bioinformatics are consistent with the NIH Director's roadmap, which emphasizes computational approaches to biology and in our infectious disease research.

Public Health Relevance

GeneLab ams to provide 1) infrastructure support for funded investigators and new grant applications by listing GeneLab as a significant strength ofthe research environment at LSU-SVM and TNPRC;2) infrastructure, training and support for NGS experimentation and data analysis allowing for collection of preliminary data and develop new data-analytic methods to support current and new R01 applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110760-01
Application #
8751060
Study Section
Special Emphasis Panel (ZGM1-TWD-C (3C))
Project Start
2014-06-02
Project End
2019-04-30
Budget Start
2014-06-02
Budget End
2015-04-30
Support Year
1
Fiscal Year
2014
Total Cost
$212,878
Indirect Cost
$67,874
Name
Louisiana State University A&M Col Baton Rouge
Department
Type
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803
Verhoeve, Victoria I; Jirakanwisal, Krit; Utsuki, Tadanobu et al. (2016) Differential Rickettsial Transcription in Bloodfeeding and Non-Bloodfeeding Arthropod Hosts. PLoS One 11:e0163769
Riley, Sean P; Fish, Abigail I; Garza, Daniel A et al. (2016) Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection. Infect Immun 84:790-7
Jambunathan, Nithya; Charles, Anu-Susan; Subramanian, Ramesh et al. (2016) Deletion of a Predicted β-Sheet Domain within the Amino Terminus of Herpes Simplex Virus Glycoprotein K Conserved among Alphaherpesviruses Prevents Virus Entry into Neuronal Axons. J Virol 90:2230-9
Shen, Li; Macnaughtan, Megan A; Frohlich, Kyla M et al. (2015) Multipart Chaperone-Effector Recognition in the Type III Secretion System of Chlamydia trachomatis. J Biol Chem 290:28141-55
Kaushal, Deepak; Foreman, Taylor W; Gautam, Uma S et al. (2015) Mucosal vaccination with attenuated Mycobacterium tuberculosis induces strong central memory responses and protects against tuberculosis. Nat Commun 6:8533
Stanfield, Brent; Kousoulas, Konstantin Gus (2015) Herpes Simplex Vaccines: Prospects of Live-attenuated HSV Vaccines to Combat Genital and Ocular infections. Curr Clin Microbiol Rep 2:125-136
Baños-Lara, Ma Del Rocío; Harvey, Lindsey; Mendoza, Alexander et al. (2015) Impact and regulation of lambda interferon response in human metapneumovirus infection. J Virol 89:730-42
Charles, Anu-Susan; Chouljenko, Vladimir N; Jambunathan, Nithya et al. (2014) Phenylalanine residues at the carboxyl terminus of the herpes simplex virus 1 UL20 membrane protein regulate cytoplasmic virion envelopment and infectious virus production. J Virol 88:7618-27
McCracken, M K; Christofferson, R C; Grasperge, B J et al. (2014) Aedes aegypti salivary protein ""aegyptin"" co-inoculation modulates dengue virus infection in the vertebrate host. Virology 468-470:133-9
Gillespie, Joseph J; Driscoll, Timothy P; Verhoeve, Victoria I et al. (2014) Genomic diversification in strains of Rickettsia felis Isolated from different arthropods. Genome Biol Evol 7:35-56

Showing the most recent 10 out of 12 publications