The Administrative Core (AC) with the approval ofthe External Advisory Committee (EAC) officially launched the Protein Core laboratory (PCL ) as an independent CEIDR Core in January 2013 to provide protein production, purification and characterization and generation of monospecific antibodies. The increasing need for protein-associated support among CEIDR scientists and the opportunity to closely collaborate with other Centralized Facilities in the LSU main campus and LSU faculty working in protein structure and function were the primary motives for launching this Core, expanding on few protein-associated functions previously offered through the Molecular Immunopathology Core (MIPC). The PCL was strategically located in the LSU main campus (Wilson Hall) immediately adjacent to the Agricultural Biotechnology laboratory that provides additional protein-associated functions including peptide synthesis and characterization for the CEIDR Protein Core laboratory. Wilson Hall is located proximal to other LSU Core Facilities offering mass spectroscopy, NMR and x-ray crystallography. The PCL provides protein production using both prokaryotic and eukaryotic expression systems, purification and characterization of proteins, generation of monospecific antibodies in mice and rabbits, and characterization of protein-protein interactions using surface plasmon resonance (SPR) and other methodologies. PCL also produces monospecific antibodies in rabbits and mice using purified protein immunogens, as well as viral vector and DNA-based immunization methods. Antibodies are concentrated and purified using standard methodologies. The laboratory seeks to continuously adapt and develop new methodologies needed by CEIDR scientists. Equipment scheduling, reagents and services are facilitated by the internet-based ilab Solutions, Inc. software providing an efficient online system for communication, ordering and evaluation ofthe Core's performance. The sen/ices provided by PCL will be of great help to all louisiana-based scientists, since there is no other similar facility in the state. Importantly, the PCL will enable collaboration between protein chemists and infectious disease researchers toward the submission of competitive NIH grant applications.

Public Health Relevance

The PCL is designed to provide basic and advanced services, equipment and consultation to enable primarily CElDR-affiliated researchers to sustain their NIH-funding and launch new NIH grant applications. PCL provides access to expensive equipment and resources as well as experienced technical support that is difficult to duplicate in individual laboratories.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1-TWD-C (3C))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Louisiana State University A&M Col Baton Rouge
Baton Rouge
United States
Zip Code
Verhoeve, Victoria I; Jirakanwisal, Krit; Utsuki, Tadanobu et al. (2016) Differential Rickettsial Transcription in Bloodfeeding and Non-Bloodfeeding Arthropod Hosts. PLoS One 11:e0163769
Riley, Sean P; Fish, Abigail I; Garza, Daniel A et al. (2016) Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection. Infect Immun 84:790-7
Jambunathan, Nithya; Charles, Anu-Susan; Subramanian, Ramesh et al. (2016) Deletion of a Predicted β-Sheet Domain within the Amino Terminus of Herpes Simplex Virus Glycoprotein K Conserved among Alphaherpesviruses Prevents Virus Entry into Neuronal Axons. J Virol 90:2230-9
Shen, Li; Macnaughtan, Megan A; Frohlich, Kyla M et al. (2015) Multipart Chaperone-Effector Recognition in the Type III Secretion System of Chlamydia trachomatis. J Biol Chem 290:28141-55
Kaushal, Deepak; Foreman, Taylor W; Gautam, Uma S et al. (2015) Mucosal vaccination with attenuated Mycobacterium tuberculosis induces strong central memory responses and protects against tuberculosis. Nat Commun 6:8533
Stanfield, Brent; Kousoulas, Konstantin Gus (2015) Herpes Simplex Vaccines: Prospects of Live-attenuated HSV Vaccines to Combat Genital and Ocular infections. Curr Clin Microbiol Rep 2:125-136
Baños-Lara, Ma Del Rocío; Harvey, Lindsey; Mendoza, Alexander et al. (2015) Impact and regulation of lambda interferon response in human metapneumovirus infection. J Virol 89:730-42
Charles, Anu-Susan; Chouljenko, Vladimir N; Jambunathan, Nithya et al. (2014) Phenylalanine residues at the carboxyl terminus of the herpes simplex virus 1 UL20 membrane protein regulate cytoplasmic virion envelopment and infectious virus production. J Virol 88:7618-27
McCracken, M K; Christofferson, R C; Grasperge, B J et al. (2014) Aedes aegypti salivary protein ""aegyptin"" co-inoculation modulates dengue virus infection in the vertebrate host. Virology 468-470:133-9
Gillespie, Joseph J; Driscoll, Timothy P; Verhoeve, Victoria I et al. (2014) Genomic diversification in strains of Rickettsia felis Isolated from different arthropods. Genome Biol Evol 7:35-56

Showing the most recent 10 out of 12 publications