The mission ofthe Protein Structure Laboratory (PSL) is to provide investigators with state-of-the-art instrumentation, facilities, and expertise for all aspects of protein crystallography. This includes protein crystallization. X-ray data collection, data analysis, structure solution and refinement, and structure analysis. Laboratory staff provides advice and consultation, training, access to facilities for experienced crystallographers, and a range of services from crystal growth to structure solution and refinement, and preparation of materials for publications and grant submissions. In addition, the PSL provides training to students, post-docs and faculty who wish to learn any or all aspects of protein structure determination. To facilitate this, modern crystallization methods, in-house or synchrotron X-ray diffraction data collection, and the most recent crystallographic software are utilized. The staff consists of one experienced full time Ph.D. crystallographer. Dr. Scott Lovell, who serves as the Director ofthe Laboratory, and a Research Assistant to assist with crystallization screening and sample preparation for X-ray diffraction experiments. The PSL is located in close proximity to other core service laboratories in the University of Kansas Structural Biology Center building. During Phase II, the PSL has collaborated with 36 investigators from academic, government and industrial institutions. This has resulted in more than 100 structures delivered, 64 deposited to the Protein Databank, 26 publications and 17 grant applications submitted in which the PSL staff were listed. The PSL will continue to expand the base of collaborators and provide high-level facilities, expertise and training during Phase III ofthe COBRE-PSF.

Public Health Relevance

The Protein Structure Core Laboratory uses X-ray crystallography to determine detailed 3-dimensional structures of proteins for investigators. It also provides training in all relevant methods used in protein crystallography.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C3))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Kansas Lawrence
United States
Zip Code
Obayashi, Eiji; Luna, Rafael E; Nagata, Takashi et al. (2017) Molecular Landscape of the Ribosome Pre-initiation Complex during mRNA Scanning: Structural Role for eIF3c and Its Control by eIF5. Cell Rep 18:2651-2663
Yadav, Rahul; Petrunak, Elyse M; Estrada, D Fernando et al. (2017) Structural insights into the function of steroidogenic cytochrome P450 17A1. Mol Cell Endocrinol 441:68-75
Yu, Jia; Lan, Lan; Lewin, Seth J et al. (2017) Identification of novel small molecule Beclin 1 mimetics activating autophagy. Oncotarget 8:51355-51369
Huang, Gaochao; Shrestha, Ruben; Jia, Kaimin et al. (2017) Enantioselective Synthesis of Dilignol Model Compounds and Their Stereodiscrimination Study with a Dye-Decolorizing Peroxidase. Org Lett 19:1820-1823
Yamanushkin, Pavel; Lu-Diaz, Michael; Edwards, Andrew et al. (2017) Directed nucleophilic addition of phenoxides to cyclopropenes. Org Biomol Chem 15:8153-8165
Petrunak, Elyse M; Rogers, Steven A; Aubé, Jeffrey et al. (2017) Structural and Functional Evaluation of Clinically Relevant Inhibitors of Steroidogenic Cytochrome P450 17A1. Drug Metab Dispos 45:635-645
Yu, Hao; Dranchak, Patricia; Li, Zhiru et al. (2017) Macrocycle peptides delineate locked-open inhibition mechanism for microorganism phosphoglycerate mutases. Nat Commun 8:14932
Ladokhin, Alexey S; Vargas-Uribe, Mauricio; Rodnin, Mykola V et al. (2017) Cellular Entry of the Diphtheria Toxin Does Not Require the Formation of the Open-Channel State by Its Translocation Domain. Toxins (Basel) 9:
Jeanne Dit Fouque, Kevin; Garabedian, Alyssa; Porter, Jacob et al. (2017) Fast and Effective Ion Mobility-Mass Spectrometry Separation of d-Amino-Acid-Containing Peptides. Anal Chem 89:11787-11794
Damalanka, Vishnu C; Kim, Yunjeong; Galasiti Kankanamalage, Anushka C et al. (2017) Design, synthesis, and evaluation of a novel series of macrocyclic inhibitors of norovirus 3CL protease. Eur J Med Chem 127:41-61

Showing the most recent 10 out of 50 publications