In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases l&ll. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization ofthe Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Program Director and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.

Public Health Relevance

This center of biomedical research excellence (COBRE) is investigating the cells that generate contractile behaviors in smooth muscle tissues. We are isolated cellular components and studying how gene expression changes in development and disease states. The COBRE provides Core laboratories and pilot projects to allow cutting-edge technologies to be applied to smooth muscle research projects at UNR.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110767-01
Application #
8735443
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Nevada Reno
Department
Type
DUNS #
City
Reno
State
NV
Country
United States
Zip Code
89557
Halim, Danny; Wilson, Michael P; Oliver, Daniel et al. (2017) Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice. Proc Natl Acad Sci U S A 114:E2739-E2747
Yanagimachi, Ryuzo; Harumi, Tatsuo; Matsubara, Hajime et al. (2017) Chemical and physical guidance of fish spermatozoa into the egg through the micropyle†,‡. Biol Reprod 96:780-799
Zhang, Ying; Tang, Chong; Yu, Tian et al. (2017) MicroRNAs control mRNA fate by compartmentalization based on 3' UTR length in male germ cells. Genome Biol 18:105
Yu, Tian; Tang, Chong; Zhang, Ying et al. (2017) Microfluidics-based digital quantitative PCR for single-cell small RNA quantification. Biol Reprod 97:490-496
Yan, Wei (2017) An interview with Magdalena Zernicka-Goetz. Biol Reprod 96:503-504
Yan, Wei (2017) Mark it for destruction: a novel role of mRNA methylation in maternal-to-zygotic transition†. Biol Reprod 96:829-830
Yan Editor-In-Chief Biology Of Reproduction, Wei (2017) piRNA-independent PIWI function in spermatogenesis and male fertility. Biol Reprod 96:1121-1123
Zhang, Xudong; Cozen, Aaron E; Liu, Ying et al. (2016) Small RNA Modifications: Integral to Function and Disease. Trends Mol Med 22:1025-1034
Yuan, Shuiqiao; Schuster, Andrew; Tang, Chong et al. (2016) Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development. Development 143:635-47
Baker, Salah A; Drumm, Bernard T; Saur, Dieter et al. (2016) Spontaneous Ca(2+) transients in interstitial cells of Cajal located within the deep muscular plexus of the murine small intestine. J Physiol 594:3317-38

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