The Fluorescence-Activated Cell Sorting/Flow Cytometry Shared Resource Laboratory (FACS/FCMSRL) is and will continue to be a state ofthe art facility to collaborate to provide analytical fluorescence-based flow cytometry and cell sorting. It will serve investigators from laboratories associated with the Thematic Focus of the COBRE, from other laboratories in the University of Nevada School of Medicine (UNSOM), from laboratories in the other Colleges of the University of Nevada Reno (UNR) and from other public and private laboratories in the area. To meet these overall objectives, there are several activities that will be performed. First, the FACS/FCMSRL will continue and extend existing activities which include the following: 1) assisting in designing, executing and analyzing flow cytometric experiments, 2) titrating and testing labeled antibodies and 3) providing laboratory and classroom training in flow cytometry. Second, the FACS/FCMSRL will work with investigators in the Thematic Focus and in the Single Cell Molecular Expression (SCME) laboratory to identify and initiate the characterization of subsets of cells in smooth muscle tissues. These cells include hematopoietic cells, smooth muscle cells, interstitial cells of Cajal, fibroblast-like cells, neuronal cells and mesothelial-like cells. Third, the Director will continue to monitor developments in flow cytometry by continuing to participate in the activities of the International Society for the Advancement of Cytometry (ISAC) by attending regular meetings and Congresses of ISAC and by participating in other ISAC-sponsored activities. Existing instruments will be upgraded as appropriate and additional instruments will be purchased if needed. Finally, during the period ofthe Phase III funding, mechanisms to provide long-term sustainability will be developed.

Public Health Relevance

It is essential for a research university to have state ofthe art flow cytometry (FCM) and fluorescence activated cell sorting (FACS) capabilities. The investigators in Physiology &Cell Biology (PCB) have been at the forefront in using FCM and FACS to characterize the cells in smooth muscle tissues. These studies will now be extended by working with the new equipment in the Single Cell Molecular Expression Laboratory.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110767-01
Application #
8735446
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Nevada Reno
Department
Type
DUNS #
City
Reno
State
NV
Country
United States
Zip Code
89557
Halim, Danny; Wilson, Michael P; Oliver, Daniel et al. (2017) Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice. Proc Natl Acad Sci U S A 114:E2739-E2747
Yanagimachi, Ryuzo; Harumi, Tatsuo; Matsubara, Hajime et al. (2017) Chemical and physical guidance of fish spermatozoa into the egg through the micropyle†,‡. Biol Reprod 96:780-799
Zhang, Ying; Tang, Chong; Yu, Tian et al. (2017) MicroRNAs control mRNA fate by compartmentalization based on 3' UTR length in male germ cells. Genome Biol 18:105
Yu, Tian; Tang, Chong; Zhang, Ying et al. (2017) Microfluidics-based digital quantitative PCR for single-cell small RNA quantification. Biol Reprod 97:490-496
Yan, Wei (2017) An interview with Magdalena Zernicka-Goetz. Biol Reprod 96:503-504
Yan, Wei (2017) Mark it for destruction: a novel role of mRNA methylation in maternal-to-zygotic transition†. Biol Reprod 96:829-830
Yan Editor-In-Chief Biology Of Reproduction, Wei (2017) piRNA-independent PIWI function in spermatogenesis and male fertility. Biol Reprod 96:1121-1123
Zhang, Xudong; Cozen, Aaron E; Liu, Ying et al. (2016) Small RNA Modifications: Integral to Function and Disease. Trends Mol Med 22:1025-1034
Yuan, Shuiqiao; Schuster, Andrew; Tang, Chong et al. (2016) Sperm-borne miRNAs and endo-siRNAs are important for fertilization and preimplantation embryonic development. Development 143:635-47
Baker, Salah A; Drumm, Bernard T; Saur, Dieter et al. (2016) Spontaneous Ca(2+) transients in interstitial cells of Cajal located within the deep muscular plexus of the murine small intestine. J Physiol 594:3317-38

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