The Fluorescence-Activated Cell Sorting/Flow Cytometry Shared Resource Laboratory (FACS/FCMSRL) is and will continue to be a state ofthe art facility to collaborate to provide analytical fluorescence-based flow cytometry and cell sorting. It will serve investigators from laboratories associated with the Thematic Focus of the COBRE, from other laboratories in the University of Nevada School of Medicine (UNSOM), from laboratories in the other Colleges of the University of Nevada Reno (UNR) and from other public and private laboratories in the area. To meet these overall objectives, there are several activities that will be performed. First, the FACS/FCMSRL will continue and extend existing activities which include the following: 1) assisting in designing, executing and analyzing flow cytometric experiments, 2) titrating and testing labeled antibodies and 3) providing laboratory and classroom training in flow cytometry. Second, the FACS/FCMSRL will work with investigators in the Thematic Focus and in the Single Cell Molecular Expression (SCME) laboratory to identify and initiate the characterization of subsets of cells in smooth muscle tissues. These cells include hematopoietic cells, smooth muscle cells, interstitial cells of Cajal, fibroblast-like cells, neuronal cells and mesothelial-like cells. Third, the Director will continue to monitor developments in flow cytometry by continuing to participate in the activities of the International Society for the Advancement of Cytometry (ISAC) by attending regular meetings and Congresses of ISAC and by participating in other ISAC-sponsored activities. Existing instruments will be upgraded as appropriate and additional instruments will be purchased if needed. Finally, during the period ofthe Phase III funding, mechanisms to provide long-term sustainability will be developed.

Public Health Relevance

It is essential for a research university to have state ofthe art flow cytometry (FCM) and fluorescence activated cell sorting (FACS) capabilities. The investigators in Physiology &Cell Biology (PCB) have been at the forefront in using FCM and FACS to characterize the cells in smooth muscle tissues. These studies will now be extended by working with the new equipment in the Single Cell Molecular Expression Laboratory.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110767-01
Application #
8735446
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Nevada Reno
Department
Type
DUNS #
City
Reno
State
NV
Country
United States
Zip Code
89557
Baker, Salah A; Drumm, Bernard T; Skowronek, Karolina E et al. (2018) Excitatory Neuronal Responses of Ca2+ Transients in Interstitial Cells of Cajal in the Small Intestine. eNeuro 5:
Heredia, Dante J; Feng, Cheng-Yuan; Agarwal, Andrea et al. (2018) Postnatal Restriction of Activity-Induced Ca2+ Responses to Schwann Cells at the Neuromuscular Junction Are Caused by the Proximo-Distal Loss of Axonal Synaptic Vesicles during Development. J Neurosci 38:8650-8665
Jorgensen, Brian G; Berent, Robyn M; Ha, Se Eun et al. (2018) DNA methylation, through DNMT1, has an essential role in the development of gastrointestinal smooth muscle cells and disease. Cell Death Dis 9:474
Chuang, Tsai-Der; Xie, Yeming; Yan, Wei et al. (2018) Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma. Fertil Steril 109:919-929
Sung, Alexander P; Tang, Jennifer J-J; Guglielmo, Michael J et al. (2018) An improved method to quantify human NK cell-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) per IgG FcR-positive NK cell without purification of NK cells. J Immunol Methods 452:63-72
Wang, Zhuqing; Lee, Sandy; Oliver, Daniel et al. (2018) Prps1l1, a testis-specific gene, is dispensable for mouse spermatogenesis. Mol Reprod Dev 85:802-804
Shi, Junchao; Ko, Eun-A; Sanders, Kenton M et al. (2018) SPORTS1.0: A Tool for Annotating and Profiling Non-coding RNAs Optimized for rRNA- and tRNA-derived Small RNAs. Genomics Proteomics Bioinformatics 16:144-151
Blanco, Luz P; Payne, Bryan L; Feyertag, Felix et al. (2018) Proteins of generalist and specialist pathogens differ in their amino acid composition. Life Sci Alliance 1:e201800017
Ben Maamar, Millissia; Sadler-Riggleman, Ingrid; Beck, Daniel et al. (2018) Alterations in sperm DNA methylation, non-coding RNA expression, and histone retention mediate vinclozolin-induced epigenetic transgenerational inheritance of disease. Environ Epigenet 4:dvy010
Zhang, Yunfang; Zhang, Xudong; Shi, Junchao et al. (2018) Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs. Nat Cell Biol 20:535-540

Showing the most recent 10 out of 42 publications