The Protein Core in the University of Kentucky Center for Molecular Medicine works with biomedical investigators to facilitate the expression, purification, and characterization of recombinant proteins and other macromolecules. It makes available equipment, instrumentation, and expertise not normally be found within individual research laboratories. The Core has fermentors, bioreactors, and tissue culture facilities for expressing recombinant protein in bacteria, yeast, insect, or mammalian cells as required. It also maintains cell cracking equipment and an extensive facility for the purification of recombinant proteins. In addition, a range of analytical instruments for biophysical characterization are available in the Core, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, analytical ultracentrifugation, and X-ray crystallography. Crystallography facilities include instruments for automated crystallization trials, in house data collection equipment, and membership in a group (SER-CAT) operating a state-of-the-art data collection facility at the Advanced Photon Source located within Argonne National Laboratory. A Core staff member maintains the equipment, provides training and, works with researchers on their individual projects. Experienced users have direct access to Core facilities, while new users are first trained by Core personnel and assisted in initial experiments. Users with more difficult projects receive additional assistance from the Core personnel. Service projects are undertaken selectively for users who lack the expertise or personnel to carry out the work on their own. The Core is used by investigators representing 50-70 research groups each year, with over 4200 individual uses of instruments or facilities documented for the first four years of Phase II COBRE funding. Outreach activities include the Core web site, talks given by Core associated faculty, individual and group training sessions, a monthly research talk series, and participation in graduate courses and an NSF REU summer program. The Core is active in obtaining new instrumentation and introducing new techniques. Transition to a selfsustaining regional facility is planned over the course of Phase III COBRE funding.
This Protein Core in the Center for Molecular Medicine provides a variety of services aimed at the production and characterization of proteins for investigator inflated biomedical research. The Core makes available well maintained instruments and facilities not generally found in individual research labs, and provides both training in their use and expert guidence. In addition, service projects carried out by Core personnel are undertaken.
|Wachter, Erin; MoyÃ¡, Diego; Parkin, Sean et al. (2016) Ruthenium Complex "Light Switches" that are Selective for Different G-Quadruplex Structures. Chemistry 22:550-9|
|Meier, Shelby; Bell, Michelle; Lyons, Danielle N et al. (2016) Pathological Tau Promotes Neuronal Damage by Impairing Ribosomal Function and Decreasing Protein Synthesis. J Neurosci 36:1001-7|
|Xu, Xuehe; Watt, David S; Liu, Chunming (2016) Multifaceted roles for thymine DNA glycosylase in embryonic development and human carcinogenesis. Acta Biochim Biophys Sin (Shanghai) 48:82-9|
|Wagner, Jonathan M; Chan, Sum; Evans, Timothy J et al. (2016) Structures of EccB1 and EccD1 from the core complex of the mycobacterial ESX-1 type VII secretion system. BMC Struct Biol 16:5|
|Sviripa, Vitaliy M; Burikhanov, Ravshan; Obiero, Josiah M et al. (2016) Par-4 secretion: stoichiometry of 3-arylquinoline binding to vimentin. Org Biomol Chem 14:74-84|
|Frasinyuk, Mykhaylo S; Mrug, Galyna P; Bondarenko, Svitlana P et al. (2016) Antineoplastic Isoflavonoids Derived from Intermediate ortho-Quinone Methides Generated from Mannich Bases. ChemMedChem 11:600-11|
|Kwiatkowski, Stefan; Sviripa, Vitaliy M; Zhang, Zhaiyi et al. (2016) Synthesis of a norcantharidin-tethered guanosine: Protein phosphatase-1 inhibitors that change alternative splicing. Bioorg Med Chem Lett 26:965-8|
|Riedmann, Caitlyn; Fondufe-Mittendorf, Yvonne N (2016) Comparative analysis of linker histone H1, MeCP2, and HMGD1 on nucleosome stability and target site accessibility. Sci Rep 6:33186|
|Falaleeva, Marina; Surface, Justin; Shen, Manli et al. (2015) SNORD116 and SNORD115 change expression of multiple genes and modify each other's activity. Gene 572:266-73|
|Kril, Liliia M; Vilchez, Valery; Jiang, Jieyun et al. (2015) N-Aryl benzenesulfonamide inhibitors of [3H]-thymidine incorporation and Î²-catenin signaling in human hepatocyte-derived Huh-7 carcinoma cells. Bioorg Med Chem Lett 25:3897-9|
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