The Protein Core in the University of Kentucky Center for Molecular Medicine works with biomedical investigators to facilitate the expression, purification, and characterization of recombinant proteins and other macromolecules. It makes available equipment, instrumentation, and expertise not normally be found within individual research laboratories. The Core has fermentors, bioreactors, and tissue culture facilities for expressing recombinant protein in bacteria, yeast, insect, or mammalian cells as required. It also maintains cell cracking equipment and an extensive facility for the purification of recombinant proteins. In addition, a range of analytical instruments for biophysical characterization are available in the Core, including dynamic light scattering, spectropolarimetry, fluorescence, isothermal titration calorimetry, high performance liquid chromatography, analytical ultracentrifugation, and X-ray crystallography. Crystallography facilities include instruments for automated crystallization trials, in house data collection equipment, and membership in a group (SER-CAT) operating a state-of-the-art data collection facility at the Advanced Photon Source located within Argonne National Laboratory. A Core staff member maintains the equipment, provides training and, works with researchers on their individual projects. Experienced users have direct access to Core facilities, while new users are first trained by Core personnel and assisted in initial experiments. Users with more difficult projects receive additional assistance from the Core personnel. Service projects are undertaken selectively for users who lack the expertise or personnel to carry out the work on their own. The Core is used by investigators representing 50-70 research groups each year, with over 4200 individual uses of instruments or facilities documented for the first four years of Phase II COBRE funding. Outreach activities include the Core web site, talks given by Core associated faculty, individual and group training sessions, a monthly research talk series, and participation in graduate courses and an NSF REU summer program. The Core is active in obtaining new instrumentation and introducing new techniques. Transition to a selfsustaining regional facility is planned over the course of Phase III COBRE funding.

Public Health Relevance

This Protein Core in the Center for Molecular Medicine provides a variety of services aimed at the production and characterization of proteins for investigator inflated biomedical research. The Core makes available well maintained instruments and facilities not generally found in individual research labs, and provides both training in their use and expert guidence. In addition, service projects carried out by Core personnel are undertaken.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110787-01
Application #
8751190
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-04-30
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Kentucky
Department
Type
DUNS #
City
Lexington
State
KY
Country
United States
Zip Code
40506
Zhang, Jinchao; Huang, Yunjie; Chen, Jing et al. (2018) Dynamic cycling of t-SNARE acylation regulates platelet exocytosis. J Biol Chem 293:3593-3606
Bodnar, Colleen N; Morganti, Josh M; Bachstetter, Adam D (2018) Depression following a traumatic brain injury: uncovering cytokine dysregulation as a pathogenic mechanism. Neural Regen Res 13:1693-1704
Frazier, H N; Anderson, K L; Maimaiti, S et al. (2018) Expression of a Constitutively Active Human Insulin Receptor in Hippocampal Neurons Does Not Alter VGCC Currents. Neurochem Res :
Sharma, Savita; Vander Kooi, Carl D; Gentry, Matthew S et al. (2018) Oligomerization and carbohydrate binding of glucan phosphatases. Anal Biochem 563:51-55
Tuukkanen, Anne T; Freire, Diana; Chan, Sum et al. (2018) Structural Variability of EspG Chaperones from Mycobacterial ESX-1, ESX-3, and ESX-5 Type VII Secretion Systems. J Mol Biol :
Lanzillotta, Chiara; Tramutola, Antonella; Meier, Shelby et al. (2018) Early and Selective Activation and Subsequent Alterations to the Unfolded Protein Response in Down Syndrome Mouse Models. J Alzheimers Dis 62:347-359
Sviripa, Vitaliy M; Kril, Liliia M; Zhang, Wen et al. (2018) Phenylethynyl-substituted Heterocycles Inhibit Cyclin D1 and Induce the Expression of Cyclin-dependent Kinase Inhibitor p21Wif1/Cip1 in Colorectal Cancer Cells. Medchemcomm 9:87-99
Rush, Jeffrey S; Edgar, Rebecca J; Deng, Pan et al. (2017) The molecular mechanism of N-acetylglucosamine side-chain attachment to the Lancefield group A carbohydrate in Streptococcus pyogenes. J Biol Chem 292:19441-19457
Wachter, Erin; Moy√°, Diego; Glazer, Edith C (2017) Combining a Ru(II) ""Building Block"" and Rapid Screening Approach to Identify DNA Structure-Selective ""Light Switch"" Compounds. ACS Comb Sci 19:85-95
Sikora, Aleksandra E; Mills, Robert H; Weber, Jacob V et al. (2017) Peptide Inhibitors Targeting the Neisseria gonorrhoeae Pivotal Anaerobic Respiration Factor AniA. Antimicrob Agents Chemother 61:

Showing the most recent 10 out of 43 publications