The Genetic Technologies Core evolved from the Viral Core that began with funding from an ARRA supplement grant in 2009 and was incorporated into the COBRE in 2011. It was initially conceived as a central service facility for the preparation, purification and titering of recombinant lentlviruses, adenoviruses and adeno-associated viruses for the use of investigators associated with the Center for Molecuair Medicine and for other investigators interested in the use of recombinant viruses as a delivery tool for nucleic acids. Over the past four years, the Core has steadily grown in the number of individual viral preparations that it produced and the number of investigators it served. There has evolved an interest from users for services in addition to those associated with viral production and the Core has adapted by increasing the scope of services provided, necessitating the change to its new designation as the Genetic Technologies Core. At present, the Genetic Technologies Core provides a broad range of services related to the manipulation and analysis of recombinant DNAs, especially for those laboratories with clinical/translational interests that are not equipped for recombinant DNA work. Evolution of the Core continues as it adds capacity in genomic manipulation through the development and use of """"""""recombineering"""""""", TALEN and piggybac technologies. These technologies will be especially valuable for the creation of cellular and animal models of human diseases that can be exploited by members of the Center for Molecular Medicine. In addition to its scientific development, the Core is also expanding its outreach programs by interfacing with the expanding Masters of Medical Science program (currently over 30 students) to provide hands-on and didactic instruction in recombinant DNA technologies, including viral production and assay, mammalian genomic manipulation, tissue culture techniques and federal regulatory and safety requirements for up to three students each semester. A parallel, didactic-only class is being developed for PhD graduate students. These courses will serve as additional revenue sources to sustain this core beyond COBRE funding.

Public Health Relevance

The Genetic Technologies Core provides a wide range of services relating to the manipulation of recombinant DNAs and mammalian genomes for investigators associated with the Center for Molecular Medicine. Of particular importance are the production of recombinant viruses for the delivery of nucleic acids for experimental use and the manipulation of mammalian genomes for creation of cell-based and animalbased human disease models.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Center Core Grants (P30)
Project #
1P30GM110787-01
Application #
8751197
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-04-30
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Kentucky
Department
Type
DUNS #
City
Lexington
State
KY
Country
United States
Zip Code
40506
Zhang, Jinchao; Huang, Yunjie; Chen, Jing et al. (2018) Dynamic cycling of t-SNARE acylation regulates platelet exocytosis. J Biol Chem 293:3593-3606
Bodnar, Colleen N; Morganti, Josh M; Bachstetter, Adam D (2018) Depression following a traumatic brain injury: uncovering cytokine dysregulation as a pathogenic mechanism. Neural Regen Res 13:1693-1704
Frazier, H N; Anderson, K L; Maimaiti, S et al. (2018) Expression of a Constitutively Active Human Insulin Receptor in Hippocampal Neurons Does Not Alter VGCC Currents. Neurochem Res :
Sharma, Savita; Vander Kooi, Carl D; Gentry, Matthew S et al. (2018) Oligomerization and carbohydrate binding of glucan phosphatases. Anal Biochem 563:51-55
Tuukkanen, Anne T; Freire, Diana; Chan, Sum et al. (2018) Structural Variability of EspG Chaperones from Mycobacterial ESX-1, ESX-3, and ESX-5 Type VII Secretion Systems. J Mol Biol :
Lanzillotta, Chiara; Tramutola, Antonella; Meier, Shelby et al. (2018) Early and Selective Activation and Subsequent Alterations to the Unfolded Protein Response in Down Syndrome Mouse Models. J Alzheimers Dis 62:347-359
Sviripa, Vitaliy M; Kril, Liliia M; Zhang, Wen et al. (2018) Phenylethynyl-substituted Heterocycles Inhibit Cyclin D1 and Induce the Expression of Cyclin-dependent Kinase Inhibitor p21Wif1/Cip1 in Colorectal Cancer Cells. Medchemcomm 9:87-99
Rush, Jeffrey S; Edgar, Rebecca J; Deng, Pan et al. (2017) The molecular mechanism of N-acetylglucosamine side-chain attachment to the Lancefield group A carbohydrate in Streptococcus pyogenes. J Biol Chem 292:19441-19457
Wachter, Erin; Moy√°, Diego; Glazer, Edith C (2017) Combining a Ru(II) ""Building Block"" and Rapid Screening Approach to Identify DNA Structure-Selective ""Light Switch"" Compounds. ACS Comb Sci 19:85-95
Sikora, Aleksandra E; Mills, Robert H; Weber, Jacob V et al. (2017) Peptide Inhibitors Targeting the Neisseria gonorrhoeae Pivotal Anaerobic Respiration Factor AniA. Antimicrob Agents Chemother 61:

Showing the most recent 10 out of 43 publications