The Cellular and Molecular Neuroscience Core is designed to facilitate the research objectives of the IDDRC and the scientific progress of individual IDDRC projects by providing state-of-the-art equipment, training, and expertise for IDDRC investigators employing, or wishing to employ, cellular and molecular biology in their research programs. In-house equipment includes advanced microscopy (laser scanning confocal and fluorescence microscopy with Stereology), genomic and proteomic analysis both in situ (flow cytometry) and in cell lysates (phosphonmaging and quantitative PCR), and informatics. During the current project period, the Waisman Center committed $500,000 of gift funds to upgrade the Core's existing proteomics and confocal microscopy capabilities. In addition, the CMN Core has well-established partnerships with nearby UW-Madison core facilities (UW-Biotechnology Center and UW-Cancer Center) to offer cost effective, state-of-the-art genomic and proteomic technology, such as 2-dimensional gel analysis, mass spectrometry, DNA sequencing and synthesis, DNA array and SNP analysis, NextGen sequencing, tiled array analysis of chromatin immunoprecipitation (ChIP) assays, and small molecule drug screening. In response to the growing interest for human-derived IDD models, in 2009 we created a new service of the CMN Core (with ARRA support) to generate induced pluripotent stem cells (iPSCs) from individuals with IDD conditions for IDDRC investigators. The IPSC service leverages the IDDRC's long-standing expertise in stem cell biology, access to well characterized patients with IDD (via the Research Participation Core), and clinical biomanufacturing (Waisman Biomanufacturing) for the production of IPSC viral vectors. Using these complementary resources, the iPSC service has successfully created 23 cell lines from patients with IDD including Rett syndrome, Alexander disease, FXS, DS, SMA, retinitis pigmentosa. Best disease, and others, and provided guidance and expertise in differentiating the cell lines into neuronal sub-types and astrocytes.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Center Core Grants (P30)
Project #
5P30HD003352-48
Application #
8734264
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
48
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
City
Madison
State
WI
Country
United States
Zip Code
53715
Lundwall, Rebecca A; Woodruff, Jason; Tolboe, Steven P (2018) RT Slowing to Valid Cues on a Reflexive Attention Task in Children and Young Adults. Front Psychol 9:1324
Hess, Christi L; Misurelli, Sara M; Litovsky, Ruth Y (2018) Spatial Release From Masking in 2-Year-Olds With Normal Hearing and With Bilateral Cochlear Implants. Trends Hear 22:2331216518775567
Laxman, D J; Greenberg, J S; DaWalt, L S et al. (2018) Medication use by adolescents and adults with fragile X syndrome. J Intellect Disabil Res 62:94-105
Li, James J; Lansford, Jennifer E (2018) A smartphone-based ecological momentary assessment of parental behavioral consistency: Associations with parental stress and child ADHD symptoms. Dev Psychol 54:1086-1098
Lundwall, Rebecca A; Sgro, Jordan F; Fanger, Julia (2018) Response time scores on a reflexive attention task predict a child's inattention score from a parent report. PLoS One 13:e0190724
Morrison, Ryann A; Martinez, Jonathan I; Hilton, Emily C et al. (2018) The influence of parents and schools on developmental trajectories of antisocial behaviors in Caucasian and African American youths. Dev Psychopathol :1-13
Patzlaff, Natalie E; Shen, Minjie; Zhao, Xinyu (2018) Regulation of Adult Neurogenesis by the Fragile X Family of RNA Binding Proteins. Brain Plast 3:205-223
Vijayan, Murali; Kumar, Subodh; Yin, Xiangling et al. (2018) Identification of novel circulatory microRNA signatures linked to patients with ischemic stroke. Hum Mol Genet 27:2318-2329
Plate, Rista C; Fulvio, Jacqueline M; Shutts, Kristin et al. (2018) Probability Learning: Changes in Behavior Across Time and Development. Child Dev 89:205-218
Hartley, Sigan L; Papp, Lauren M; Bolt, Daniel (2018) Spillover of Marital Interactions and Parenting Stress in Families of Children With Autism Spectrum Disorder. J Clin Child Adolesc Psychol 47:S88-S99

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