Increasingly sophisticated methods to visualize tissue/cellular structure/neuronal circuits and to monitor real-time molecular interactions and biochemical reactions, have contributed to recent progress both in developmental neurobiology and in studying neurodevelopmental disorders. Current advancement has been augmented by access to specific antibodies for immunostaining, cloning of nucleic acids and in situ hybridization, new dyes and cell labeling techniques, in combination with a diverse array of powerful new microscopes. Imaging technologies such as laser scanning confocal microscopy, multiphoton excitation fluorescence, fluorescence recovery after photobleaching (FRAP), fluorescence resonance energy transfer (FRET), Spinning Disk Confocal microscopy and Array Tomography offer greatly increased resolution of cellular processes and require sophisticated analysis programs. However, the cost of purchase and maintenance of these instruments exceeds the budgets of most individual laboratories. The objective of the Cellular imaging Core, which is the result of the combination of two former IDDRC Cores, is to provide affordable access to state-of-the-art equipment. services, training and advice for projects using histological and imaging techniques. This new core, comprised of three components. Histology, Microscopy and Image Analysis, will build on the strengths and experience of the former cores, by providing a much more integrated set of services and capabilities. The Core continuously explores new technologies and has invested heavily in new equipment and image analysis software during the past grant period. As a result of these investments, the Core has greatly expanded its'repertoire of tools to visualize and analyze cellular processes, enabling IDDRC investigators to take their research to new levels. We are committed to maintaining this core as a central resource for neuroscience research at CHB and Harvard Medical School.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Center Core Grants (P30)
Project #
5P30HD018655-32
Application #
8509728
Study Section
Special Emphasis Panel (ZHD1-DSR-Y)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
32
Fiscal Year
2013
Total Cost
$226,426
Indirect Cost
$96,297
Name
Children's Hospital Boston
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115
Soto-Rivera, Carmen L; Fichorova, Raina N; Allred, Elizabeth N et al. (2015) The relationship between TSH and systemic inflammation in extremely preterm newborns. Endocrine 48:595-602
Duffy, Frank H; Shankardass, Aditi; McAnulty, Gloria B et al. (2014) Corticosteroid therapy in regressive autism: a retrospective study of effects on the Frequency Modulated Auditory Evoked Response (FMAER), language, and behavior. BMC Neurol 14:70
O'Shea, T Michael; Joseph, Robert M; Kuban, Karl C K et al. (2014) Elevated blood levels of inflammation-related proteins are associated with an attention problem at age 24 mo in extremely preterm infants. Pediatr Res 75:781-7
Mellado Lagarde, Marcia M; Wan, Guoqiang; Zhang, LingLi et al. (2014) Spontaneous regeneration of cochlear supporting cells after neonatal ablation ensures hearing in the adult mouse. Proc Natl Acad Sci U S A 111:16919-24
Pekkurnaz, Gulcin; Trinidad, Jonathan C; Wang, Xinnan et al. (2014) Glucose regulates mitochondrial motility via Milton modification by O-GlcNAc transferase. Cell 158:54-68
Kong, Sek Won; Sahin, Mustafa; Collins, Christin D et al. (2014) Divergent dysregulation of gene expression in murine models of fragile X syndrome and tuberous sclerosis. Mol Autism 5:16
Logan, J Wells; Allred, Elizabeth N; Fichorova, Raina N et al. (2014) Endogenous erythropoietin varies significantly with inflammation-related proteins in extremely premature newborns. Cytokine 69:22-8
Painter, Michio W; Brosius Lutz, Amanda; Cheng, Yung-Chih et al. (2014) Diminished Schwann cell repair responses underlie age-associated impaired axonal regeneration. Neuron 83:331-43
Bankova, Lora G; Lezcano, Cecilia; Pejler, Gunnar et al. (2014) Mouse mast cell proteases 4 and 5 mediate epidermal injury through disruption of tight junctions. J Immunol 192:2812-20
Singh, Sasha A; Winter, Dominic; Kirchner, Marc et al. (2014) Co-regulation proteomics reveals substrates and mechanisms of APC/C-dependent degradation. EMBO J 33:385-99

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