The initial (1999) application for this IDDRC included separate cores in Neuropathology and Neuroscience, with the latter offering expertise in cell culture. As user needs evolved, it became apparent that these facilities should be merged into a single Cellular Neuroscience Core. This was accomplished in 1994 with Dr. Pleasure as the Director. He served in this capacity unfil 2001, when leadership passed to Dr. Jeffrey Golden, a pediatric neuropathologist with a primary interest in developmental disorders. Dr. Golden first joined CHOP in 1997 and immediately became engaged with the IDDRC, both as a user and as an Associate Director of the Cellular Neuroscience Core. Since its inception this Core has provided users with a diverse repertoire of state-of-the-art methods for visualizaion of the distribufions of gene products in normal, developing neural cells and in those undergoing various forms of degeneration and regenerafion. We have confinually and eagerly added new skills, instrumentafion and reagents to better serve our users'needs. In 1995, with the generous assistance of the Children's Hospital, we purchased a Leica confocal microscope to which we added inverted microscopy, stagemounted micromanipulators/microinjectors, and a stage-mounted environmental chamber, thus permitting prolonged observation and manipulation of living cells under physiological conditions. In this manner we offered 8-color capacity fluorescent imaging. Our institution paid for the apparatus and we used IDDRC funding to partially support a technician. We made several other notable technological additions to the Core repertoire, including in situ hybridization in both sections and whole embryos. Our general purpose has been not only to make available a technology, but a consultative sen/ice that facilitates implementation of the method as well as the interpretation of data. We adhered to this policy when we also added video-enhanced microscopy in order to better support IDDRC investigators in analyzing intracellular Ca2+ and Na+ as well as the estimate of mitochondrial potential.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Center Core Grants (P30)
Project #
5P30HD026979-24
Application #
8523938
Study Section
Special Emphasis Panel (ZHD1-MRG-C)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
24
Fiscal Year
2013
Total Cost
$160,449
Indirect Cost
$42,176
Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Ghanbari, Yasser; Bloy, Luke; Christopher Edgar, J et al. (2015) Joint analysis of band-specific functional connectivity and signal complexity in autism. J Autism Dev Disord 45:444-60
Howerton, Alexis R; Roland, Alison V; Bale, Tracy L (2014) Dorsal raphe neuroinflammation promotes dramatic behavioral stress dysregulation. J Neurosci 34:7113-23
Lin, Hong; Hsu, Fu-Chun; Baumann, Bailey H et al. (2014) Cortical parvalbumin GABAergic deficits with ?7 nicotinic acetylcholine receptor deletion: implications for schizophrenia. Mol Cell Neurosci 61:163-75
Sunnen, C Nicole; Simonet, Jacqueline C; Marsh, Eric D et al. (2014) Arx is required for specification of the zona incerta and reticular nucleus of the thalamus. J Neuropathol Exp Neurol 73:253-61
Jackson, Joshua G; O'Donnell, John C; Takano, Hajime et al. (2014) Neuronal activity and glutamate uptake decrease mitochondrial mobility in astrocytes and position mitochondria near glutamate transporters. J Neurosci 34:1613-24
Lysko, Daniel E; Putt, Mary; Golden, Jeffrey A (2014) SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules. J Neurosci 34:4941-62
Zhang, Zhe; Hailat, Zeyad; Falk, Marni J et al. (2014) Integrative analysis of independent transcriptome data for rare diseases. Methods 69:315-25
Schrier Vergano, Samantha; Rao, Meera; McCormack, Shana et al. (2014) In vivo metabolic flux profiling with stable isotopes discriminates sites and quantifies effects of mitochondrial dysfunction in C. elegans. Mol Genet Metab 111:331-41
Brennan, Jonathan; Lignos, Constantine; Embick, David et al. (2014) Spectro-temporal correlates of lexical access during auditory lexical decision. Brain Lang 133:39-46
Jager, Jennifer; O'Brien, W Timothy; Manlove, Jessica et al. (2014) Behavioral changes and dopaminergic dysregulation in mice lacking the nuclear receptor Rev-erb?. Mol Endocrinol 28:490-8

Showing the most recent 10 out of 250 publications