The goal of the Cell and Molecular Imaging (CMI) Core is to provide RFK-IDDRC investigators with a comprehensive package of expert guidance, training and assistance in optical, electron and related microscopy techniques and image processing, together with access to costly state-of-the-art equipment. It is expected that the vast majority of IDDRC investigators will make use of this Core given its widespread and fundamental applicability to biological studies. This Core, previously known as the Morphology Core, has existed at Einstein for over 20 years, and has grown from two widefield light microscopes and one electron microscope to a compendium of multiple facilities. Its recognized broad utility to investigators also garnered generous financial support from the Department of Neuroscience, both to sustain personnel as well as to provide for purchase of additional equipment during the absence of NICHD-sponsored center funding. This permitted continued Core growth and uninterrupted service to investigators both inside and outside of the Kennedy Center. Core resources include specialized widefield microscopes and electron microscopes that are utilized for a wide array of specimens and purposes by IDDRC investigators. Samples come from a variety of animal species and include cells, tissue sections or slices, organotypic preparations or whole organisms. Specific microscopy goals for IDDRC investigators are diverse and include studies focused on areas such as cell organization or cytoarchitecture, intracellular organelle distribution, and changes in pathologic markers. Live cell or tissue imaging studies are also carried out, including tracer studies for analysis of organelle transport and turnover. All studies benefit from expert advice and assistance from experienced Core personnel followed by comprehensive image acquisition and analysis. Utility of the Core is further extended by its provision of equipment for high-resolution laser-assisted dissection for gene expression and proteomic studies on tissue and cell preparations, and biolistic technology for facile delivery of naked DNA constructs. The goal is not only to facilitate high-end equipment use, but also to educate and provide highly personalized advice and support to investigators on all relevant methods from initial specimen preparation to final packaging of data for publication or presentation.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Center Core Grants (P30)
Project #
5P30HD071593-04
Application #
8734924
Study Section
Special Emphasis Panel (ZHD1-DSR-Y)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
4
Fiscal Year
2014
Total Cost
$152,536
Indirect Cost
$61,197
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Goldman, Sylvie; DeNigris, Danielle (2015) Parents' strategies to elicit autobiographical memories in autism spectrum disorders, developmental language disorders and typically developing children. J Autism Dev Disord 45:1464-73
Hahn, Noemi; Foxe, John J; Molholm, Sophie (2014) Impairments of multisensory integration and cross-sensory learning as pathways to dyslexia. Neurosci Biobehav Rev 47:384-92
Negoro, Hiromitsu; Urban-Maldonado, Marcia; Liou, Louis S et al. (2014) Pannexin 1 channels play essential roles in urothelial mechanotransduction and intercellular signaling. PLoS One 9:e106269
Altschuler, Ted S; Molholm, Sophie; Butler, John S et al. (2014) The effort to close the gap: tracking the development of illusory contour processing from childhood to adulthood with high-density electrical mapping. Neuroimage 90:360-73
O'Guin, Kathleen N; Gruber, Ross C; Raine, Cedric S et al. (2014) Gas6 enhances axonal ensheathment by MBP+ membranous processes in human DRG/OL promyelinating co-cultures. ASN Neuro 6:e00135
Qureshi, Irfan A; Mehler, Mark F (2014) Epigenetic mechanisms underlying the pathogenesis of neurogenetic diseases. Neurotherapeutics 11:708-20
Liu, Xingyin; Greer, Christina; Secombe, Julie (2014) KDM5 interacts with Foxo to modulate cellular levels of oxidative stress. PLoS Genet 10:e1004676
Berko, Esther R; Suzuki, Masako; Beren, Faygel et al. (2014) Mosaic epigenetic dysregulation of ectodermal cells in autism spectrum disorder. PLoS Genet 10:e1004402
Nguyen, Giang D; Gokhan, Solen; Molero, Aldrin E et al. (2014) The role of H1 linker histone subtypes in preserving the fidelity of elaboration of mesendodermal and neuroectodermal lineages during embryonic development. PLoS One 9:e96858
Foxe, John J; Murphy, Jeremy W; De Sanctis, Pierfilippo (2014) Throwing out the rules: anticipatory alpha-band oscillatory attention mechanisms during task-set reconfigurations. Eur J Neurosci 39:1960-72

Showing the most recent 10 out of 48 publications