In order to support the goals and of the CHAIN Center and its investigators advanced mass spectrometric based analyses are needed.
The aims proposed in the CHAIN Center cover a broad range of critical topics including biomarker discovery, assessment of the role of macrophages in neuronal damage, and the need for CNS penetrating antiviral drugs. These invesfigations are planned in order to advance our knowledge, prevention and treatment of neuroAIDS in the now chronic setting of HIV infection, and its relationship to aging. Here we will provide state of the art proteomic and metabolomics technologies to enable the identification, quantification, and correlation of the broad range of proteins and metabolites relevant to neuroAIDS. Meeting this challenge is integral to understanding the now altered progression of disease and the development of diagnostic and treatment tools. Our methods and analyses will permit the identification of specific proteins and metabolites associated with neuroAIDS and its models. One of expectations is that results of proteomics experiments will be integrated with metabolomics data, and through the use of analysis tools in this Core, the Bioinformatics subunit of the Administrative Core and the Systems Biology Core a collaborative effort will result in integrated data collection, sharing and analysis capabilities that are simultaneously divers and integrated, not only enabling our investigators to meet their scientific aims but generating a unique resource for neuroAIDS. This will be accomplished within the Core and Center as a whole and is directly applicable to in vitro, in vivo and ex vivo clinical translational studies performed in the examination of the effects of HIV on the CNS.
The Specific Aims are designed to meet the Center's Action Plan, and the two Core Directors not only bring significant expertise in proteomics and metabolomics but also have a good understanding of HIV and its neurobiology, and experience in neuroAIDS studies, which facilitate the likelihood of success of CHAIN Center investigators. Analytical instrumentation and scientific capabilities are complementary among proteomics and metabolomics components of this Core. Both directors will promote and facilitate communication between the CHAIN Center investigators and the cores to insure that the most optimal experimental approaches and instrumentation will be used.
|Kelso, Matthew L; Elliott, Bret R; Haverland, Nicole A et al. (2015) Granulocyte-macrophage colony stimulating factor exerts protective and immunomodulatory effects in cortical trauma. J Neuroimmunol 278:162-73|
|Wilson, Tony W; Heinrichs-Graham, Elizabeth; Becker, Katherine M et al. (2015) Multimodal neuroimaging evidence of alterations in cortical structure and function in HIV-infected older adults. Hum Brain Mapp 36:897-910|
|Gendelman, Howard E; Gelbard, Harris A (2014) Adjunctive and long-acting nanoformulated antiretroviral therapies for HIV-associated neurocognitive disorders. Curr Opin HIV AIDS 9:585-90|
|Johnson, Caroline H; Fisher, Timothy S; Hoang, Linh T et al. (2014) Luciferase does not Alter Metabolism in Cancer Cells. Metabolomics 10:354-360|
|Guo, Dongwei; Li, Tianyuzi; McMillan, JoEllyn et al. (2014) Small magnetite antiretroviral therapeutic nanoparticle probes for MRI of drug biodistribution. Nanomedicine (Lond) 9:1341-52|
|Sabouri, Amir H; Marcondes, Maria Cecilia Garibaldi; Flynn, Claudia et al. (2014) TLR signaling controls lethal encephalitis in WNV-infected brain. Brain Res 1574:84-95|
|Patti, Gary J; Tautenhahn, Ralf; Johannsen, Darcy et al. (2014) Meta-analysis of global metabolomic data identifies metabolites associated with life-span extension. Metabolomics 10:737-743|
|Haverland, Nicole A; Fox, Howard S; Ciborowski, Pawel (2014) Quantitative proteomics by SWATH-MS reveals altered expression of nucleic acid binding and regulatory proteins in HIV-1-infected macrophages. J Proteome Res 13:2109-19|
|Rinehart, Duane; Johnson, Caroline H; Nguyen, Thomas et al. (2014) Metabolomic data streaming for biology-dependent data acquisition. Nat Biotechnol 32:524-7|
|Villeneuve, Lance M; Stauch, Kelly L; Fox, Howard S (2014) Proteomic analysis of the mitochondria from embryonic and postnatal rat brains reveals response to developmental changes in energy demands. J Proteomics 109:228-39|
Showing the most recent 10 out of 184 publications