This core will provide Biophysical based assessments for CHAIN investigators. Both state-of-the-art Bioimaging, including quantitative magnetic resonance imaging (MRI), MR spectroscopy (MRS) and single photon emission computed tomography (SPECT), and Electrophysiological methods, including patch clamping and extracellular analysis synaptic activity. Two 7-Tesla small animal MRl/S systems will provide quantitative neuroimaging and/or superparamagnetic iron oxide (SPIO) labeled cell tracking on rodent models of HlV-1 associated neurodegenerative disorder (HAND). The bioimaging core methods also include quantitative mapping of blood-brain barrier permeability, quantitative arterial spin labeled perfusion mapping, and quantitative proton MRS (^H MRS). The electrophysiology equipment and expertise will allow investigators access to state-of-the-art in vitro and in vivo approaches for studying how immune deficiency virus, viral products, cytokines, and cytotoxins, as well as genetic over- or under-expression of these elements alter molecular, cellular and synaptic physiology of neurons and brain regions believed to be involved in neuroAIDS. The techniques in the Core as a whole will also support developmental therapeutic studies relevant to microglial activation in HAD. The results obtained from this core will have direct applicability for determining the mechanisms and monitoring the course of HIV infection in its chronic stage. Our overriding goal is to assist CHAIN Pis and other researchers interested in neuroAIDS in determining and characterizing changes of CNS function as they develop in the various in vitro and in vivo models of neuroAIDS, and in exploring therapeutic potentials aiming at ameliorating or reversing such functional changes.

Public Health Relevance

Interdisciplinary studies are key to the analysis of complex diseases such as HIV infection of the brain. This core will enable CHAIN scientists from a variety of fields to access state-of-the-art bioimaging and neurophysiological technoligies to meet the aims ofthe Center.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Center Core Grants (P30)
Project #
5P30MH062261-14
Application #
8629783
Study Section
Special Emphasis Panel (ZMH1-ERB-M)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
14
Fiscal Year
2014
Total Cost
$211,075
Indirect Cost
$68,937
Name
University of Nebraska Medical Center
Department
Type
DUNS #
168559177
City
Omaha
State
NE
Country
United States
Zip Code
68198
Villeneuve, Lance M; Purnell, Phillip R; Stauch, Kelly L et al. (2016) HIV-1 transgenic rats display mitochondrial abnormalities consistent with abnormal energy generation and distribution. J Neurovirol :
Li, Weizhe; Tong, Hsin-I; Gorantla, Santhi et al. (2016) Neuropharmacologic Approaches to Restore the Brain's Microenvironment. J Neuroimmune Pharmacol 11:484-94
Singh, Dhirender; McMillan, JoEllyn; Hilaire, James et al. (2016) Development and characterization of a long-acting nanoformulated abacavir prodrug. Nanomedicine (Lond) 11:1913-27
Sajja, Balasrinivasa R; Bade, Aditya N; Zhou, Biyun et al. (2016) Generation and Disease Model Relevance of a Manganese Enhanced Magnetic Resonance Imaging-Based NOD/scid-IL-2Rγc(null) Mouse Brain Atlas. J Neuroimmune Pharmacol 11:133-41
Jaeger, Philipp A; Lucin, Kurt M; Britschgi, Markus et al. (2016) Network-driven plasma proteomics expose molecular changes in the Alzheimer's brain. Mol Neurodegener 11:31
Bade, Aditya N; Gorantla, Santhi; Dash, Prasanta K et al. (2016) Manganese-Enhanced Magnetic Resonance Imaging Reflects Brain Pathology During Progressive HIV-1 Infection of Humanized Mice. Mol Neurobiol 53:3286-97
Zhang, Gang; Guo, Dongwei; Dash, Prasanta K et al. (2016) The mixed lineage kinase-3 inhibitor URMC-099 improves therapeutic outcomes for long-acting antiretroviral therapy. Nanomedicine 12:109-22
Burns, Ariel; Ciborowski, Pawel (2016) Acute exposure to methamphetamine alters TLR9-mediated cytokine expression in human macrophage. Immunobiology 221:199-207
Yang, Lu; Yao, Honghong; Chen, Xufeng et al. (2016) Role of Sigma Receptor in Cocaine-Mediated Induction of Glial Fibrillary Acidic Protein: Implications for HAND. Mol Neurobiol 53:1329-42
Dong, Weiguo; Embury, Christine M; Lu, Yaman et al. (2016) The mixed-lineage kinase 3 inhibitor URMC-099 facilitates microglial amyloid-β degradation. J Neuroinflammation 13:184

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