This core will provide Biophysical based assessments for CHAIN investigators. Both state-of-the-art Bioimaging, including quantitative magnetic resonance imaging (MRI), MR spectroscopy (MRS) and single photon emission computed tomography (SPECT), and Electrophysiological methods, including patch clamping and extracellular analysis synaptic activity. Two 7-Tesla small animal MRl/S systems will provide quantitative neuroimaging and/or superparamagnetic iron oxide (SPIO) labeled cell tracking on rodent models of HlV-1 associated neurodegenerative disorder (HAND). The bioimaging core methods also include quantitative mapping of blood-brain barrier permeability, quantitative arterial spin labeled perfusion mapping, and quantitative proton MRS (^H MRS). The electrophysiology equipment and expertise will allow investigators access to state-of-the-art in vitro and in vivo approaches for studying how immune deficiency virus, viral products, cytokines, and cytotoxins, as well as genetic over- or under-expression of these elements alter molecular, cellular and synaptic physiology of neurons and brain regions believed to be involved in neuroAIDS. The techniques in the Core as a whole will also support developmental therapeutic studies relevant to microglial activation in HAD. The results obtained from this core will have direct applicability for determining the mechanisms and monitoring the course of HIV infection in its chronic stage. Our overriding goal is to assist CHAIN Pis and other researchers interested in neuroAIDS in determining and characterizing changes of CNS function as they develop in the various in vitro and in vivo models of neuroAIDS, and in exploring therapeutic potentials aiming at ameliorating or reversing such functional changes.
Interdisciplinary studies are key to the analysis of complex diseases such as HIV infection of the brain. This core will enable CHAIN scientists from a variety of fields to access state-of-the-art bioimaging and neurophysiological technoligies to meet the aims ofthe Center.
|Kelso, Matthew L; Elliott, Bret R; Haverland, Nicole A et al. (2015) Granulocyte-macrophage colony stimulating factor exerts protective and immunomodulatory effects in cortical trauma. J Neuroimmunol 278:162-73|
|Wilson, Tony W; Heinrichs-Graham, Elizabeth; Becker, Katherine M et al. (2015) Multimodal neuroimaging evidence of alterations in cortical structure and function in HIV-infected older adults. Hum Brain Mapp 36:897-910|
|Gendelman, Howard E; Gelbard, Harris A (2014) Adjunctive and long-acting nanoformulated antiretroviral therapies for HIV-associated neurocognitive disorders. Curr Opin HIV AIDS 9:585-90|
|Johnson, Caroline H; Fisher, Timothy S; Hoang, Linh T et al. (2014) Luciferase does not Alter Metabolism in Cancer Cells. Metabolomics 10:354-360|
|Guo, Dongwei; Li, Tianyuzi; McMillan, JoEllyn et al. (2014) Small magnetite antiretroviral therapeutic nanoparticle probes for MRI of drug biodistribution. Nanomedicine (Lond) 9:1341-52|
|Sabouri, Amir H; Marcondes, Maria Cecilia Garibaldi; Flynn, Claudia et al. (2014) TLR signaling controls lethal encephalitis in WNV-infected brain. Brain Res 1574:84-95|
|Patti, Gary J; Tautenhahn, Ralf; Johannsen, Darcy et al. (2014) Meta-analysis of global metabolomic data identifies metabolites associated with life-span extension. Metabolomics 10:737-743|
|Haverland, Nicole A; Fox, Howard S; Ciborowski, Pawel (2014) Quantitative proteomics by SWATH-MS reveals altered expression of nucleic acid binding and regulatory proteins in HIV-1-infected macrophages. J Proteome Res 13:2109-19|
|Rinehart, Duane; Johnson, Caroline H; Nguyen, Thomas et al. (2014) Metabolomic data streaming for biology-dependent data acquisition. Nat Biotechnol 32:524-7|
|Villeneuve, Lance M; Stauch, Kelly L; Fox, Howard S (2014) Proteomic analysis of the mitochondria from embryonic and postnatal rat brains reveals response to developmental changes in energy demands. J Proteomics 109:228-39|
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