BASIC SCIENCE CORE II: ANIMAL MODEL, BEHAVIORAL TESTING, AND HISTOLOGY Basic Science Core II (BSCII) will focus on in vivo and translational aspects of basic neuroscience and will encompass animal models and their behavioral testing, histology, microscopy, and neuropathology. The BSCII will assist investigators with animal models and behavioral testing allowing them to overcome the challenges of developing and characterizing new rodent models, particularly with behavioral phenotypes, which may be outside their areas of expertise. The core will offer the most refined models of NeuroAIDS currently available including immunodeficient mouse models of HIV encephalopathy, inducible and tissue specific transgenic, knockdown, or knockout models, cell transplantation models, viral vector delivery, etc. Expertise in behavioral testing for cognitive changes in learning and memory as well as peripheral neuropathies will be provided. The experience available in breeding and phenotyping of neurological models, and behavioral testing which can be particularly challenging, will be an invaluable resource. Assistance will also be provided with histological and immunohistochemical evaluation of human autopsy and biopsy clinical samples from patients with neurological disorders including AIDS, PML, neurodegenerative diseases, and CNS neoplasia, tissues harvested from experimental animal models, and cell cultures. The core will provide neuropathological evaluation, study design and data interpretation, and microscopy services including Laser Capture Microdissection (LCM) accessioning of tissue samples and nucleic acid extraction for downstream molecular biological applications and two-photon (2P) excitation microscopy of cells and tissues for high resolution microscopic analysis. These services will assist investigators in the neuroAIDS community with histopatholgical analysis and translational studies. The core will also provide the necessary training to the investigators and their staff to gain the proficiency needed to independently conduct any of the routine core functions.

Public Health Relevance

This core will assist researchers studying AIDS and brain disorders to utilize animal models for evaluating therapeutics and behavioral tests for improving treatments diagnosis and prevention. Moreover, this core will provide services to researchers in analyzing human samples and animal tissues for improving diagnosis of disease and understanding the mechanism involved in the pathogenesis of neuroAIDS.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Center Core Grants (P30)
Project #
5P30MH092177-04
Application #
8668160
Study Section
Special Emphasis Panel (ZMH1-ERB-F)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$353,312
Indirect Cost
$153,561
Name
Temple University
Department
Type
DUNS #
057123192
City
Philadelphia
State
PA
Country
United States
Zip Code
19122
Antell, Gregory C; Dampier, Will; Aiamkitsumrit, Benjamas et al. (2017) Evidence of Divergent Amino Acid Usage in Comparative Analyses of R5- and X4-Associated HIV-1 Vpr Sequences. Int J Genomics 2017:4081585
Dampier, Will; Antell, Gregory C; Aiamkitsumrit, Benjamas et al. (2017) Specific amino acids in HIV-1 Vpr are significantly associated with differences in patient neurocognitive status. J Neurovirol 23:113-124
Sariyer, Rahsan; De-Simone, Francesca I; Donadoni, Martina et al. (2017) Alcohol-Mediated Missplicing of Mcl-1 Pre-mRNA is Involved in Neurotoxicity. Alcohol Clin Exp Res 41:1715-1724
Tahrir, Farzaneh G; Knezevic, Tijana; Gupta, Manish K et al. (2017) Evidence for the Role of BAG3 in Mitochondrial Quality Control in Cardiomyocytes. J Cell Physiol 232:797-805
Dampier, Will; Sullivan, Neil T; Chung, Cheng-Han et al. (2017) Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants. Sci Rep 7:14413
Sami Saribas, A; Cicalese, Stephanie; Ahooyi, Taha Mohseni et al. (2017) HIV-1 Nef is released in extracellular vesicles derived from astrocytes: evidence for Nef-mediated neurotoxicity. Cell Death Dis 8:e2542
Gupta, Manish K; Kaminski, Rafal; Mullen, Brian et al. (2017) HIV-1 Nef-induced cardiotoxicity through dysregulation of autophagy. Sci Rep 7:8572
Ahooyi, Taha Mohseni; Shekarabi, Masoud; Decoppet, Emilie A et al. (2017) Network Analysis of Hippocampal Neurons by Microelectrode Array in the Presence of HIV-1 Tat and Cocaine. J Cell Physiol :
O'Connor, E E; Jaillard, A; Renard, F et al. (2017) Reliability of White Matter Microstructural Changes in HIV Infection: Meta-Analysis and Confirmation. AJNR Am J Neuroradiol 38:1510-1519
Gannon, Patrick J; Akay-Espinoza, Cagla; Yee, Alan C et al. (2017) HIV Protease Inhibitors Alter Amyloid Precursor Protein Processing via ?-Site Amyloid Precursor Protein Cleaving Enzyme-1 Translational Up-Regulation. Am J Pathol 187:91-109

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