This is a proposal to continue NINDS funding of a Core Facility at Brandeis University. This will allow us to fully exploit the three subcomponents that are now firmly established: a microarray and FACS facility, an imaging facility and a mouse/transgenic facility. They are the underpinnings of a large number of NINDS- funded and other neuroscience-relevant research projects on campus. We also propose major expansions of all three existing cores and the establishment of a fourth core. The microarray/FACS facility will be expanded to encompass proteomics thereby becoming the Genomics/Proteomics Core Facility. The imaging facility will be expanded to include Correlated Light and Electron Microscopy (CLEM) and ultra high- resolution cryo-fluorescence imaging thereby become the Imaging/CLEM Core Facility. The mouse/transgenic facility has recently added the capacity to produce Lentiviral vectors for transfection and transgenesis, and is now referred to as the Transgenic Mouse and Viral Transfection Core Facility. Finally, we will establish a new Computational Core Facility to support large scale neural simulations and computational biology projects as part of a large high-performance computing cluster. These additions will allow the Brandeis community to remain at the cutting edge technologically, and ensure that we can continue to generate exciting and ground-breaking new science. The projects supported by the cores are joined together through the shared interests of multiple neuroscience faculty members in basic as well disease- related aspects of brain and neuron function: cell identity, synaptic transmission and circuits, plasticity, behavior and its modulation. The proposed studies will exploit vertebrate and invertebrate model systems, with a strong emphasis on transgenic animals. They address basic and applied problems that are pertinent to a wide range of neurological and psychiatric diseases including disturbances of excitability, such as epilepsy, disturbances of sleep, waking and mood, neurodevelopmental disorders such as Autism and Rett Syndrome, neurodegenerative disorders, like Amyotrophic Lateral Sclerosis, and disturbances of long and short -term memory such as those that accompany Alzheimer's Disease and Schizophrenia.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045713-10
Application #
8387998
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Program Officer
Talley, Edmund M
Project Start
2003-03-01
Project End
2014-11-30
Budget Start
2012-12-01
Budget End
2014-11-30
Support Year
10
Fiscal Year
2013
Total Cost
$755,634
Indirect Cost
$273,134
Name
Brandeis University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
Matts, Jessica A; Sytnikova, Yuliya; Chirn, Gung-Wei et al. (2014) Small RNA library construction from minute biological samples. Methods Mol Biol 1093:123-36
Rosado, Michelle; Barber, Cynthia F; Berciu, Cristina et al. (2014) Critical roles for multiple formins during cardiac myofibril development and repair. Mol Biol Cell 25:811-27
Ghiretti, Amy E; Moore, Anna R; Brenner, Rebecca G et al. (2014) Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo. J Neurosci 34:392-407
Moore, Anna R; Ghiretti, Amy E; Paradis, Suzanne (2013) A loss-of-function analysis reveals that endogenous Rem2 promotes functional glutamatergic synapse formation and restricts dendritic complexity. PLoS One 8:e74751
Hall, Sarah E; Chirn, Gung-Wei; Lau, Nelson C et al. (2013) RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans. RNA 19:306-19
Kuzirian, Marissa S; Moore, Anna R; Staudenmaier, Emily K et al. (2013) The class 4 semaphorin Sema4D promotes the rapid assembly of GABAergic synapses in rodent hippocampus. J Neurosci 33:8961-73
Perrat, Paola N; DasGupta, Shamik; Wang, Jie et al. (2013) Transposition-driven genomic heterogeneity in the Drosophila brain. Science 340:91-5
Ghiretti, Amy E; Kenny, Katelyn; Marr 2nd, Michael T et al. (2013) CaMKII-dependent phosphorylation of the GTPase Rem2 is required to restrict dendritic complexity. J Neurosci 33:6504-15
Luther, J A; Enes, J; Birren, S J (2013) Neurotrophins regulate cholinergic synaptic transmission in cultured rat sympathetic neurons through a p75-dependent mechanism. J Neurophysiol 109:485-96
Ghiretti, Amy E; Paradis, Suzanne (2011) The GTPase Rem2 regulates synapse development and dendritic morphology. Dev Neurobiol 71:374-89

Showing the most recent 10 out of 46 publications