Brandeis has a long and rich history of neurogenetics in invertebrate model organisms, but only in the last two years, have we begun to begin to take full advantage of existing transgenic mouse strains and develop new ones ourselves. Prior mammalian work in the Nelson, Turrigiano, Birren and Lisman laboratories has been primarily conducted in rats. Thanks to establishment of the Mouse/Virus Core facility powerful new knockout and transgenic approaches in mice are now part of the research program in each of these laboratories, and these state-of-the-art approaches have engendered new collaborations between Brandeis faculty. For example, Birren and Sengupta are studying signaling associated with the transcription factor Arx in worms and mice, and they have begun work on a conditional knockout of Arx in mice. The Nelson lab has generated new transgenic lines which allow labeling and manipulation of specific forebrain cell types. The first characterized line labels layer 4 star-pyramid neurons in primary sensory cortices. It will enhance a collaborative project with Turrigiano on the molecular basis of changing forms of synaptic plasticity in layer 4 of visual cortex. The new mice have been made using lentiviral transgenesis and reflect the facilities newly developed capability to creat and harvest lentiviral vectors for brain transfection and transgenesis. In order to optimize delivery of lentivirus to delicate tissues we proposed to purchase a Burliegh Piezo Impact Drill system. Newer additions to the Brandeis faculty will also make use of the facility. Katz is adapting his rat multielectrode recording techniques to mice and will collaborate in multiple projects with Birren, Nelson and Turrigiano on in vivo analyses of transgenic mouse models. Paradis will join the faculty in January 2008;she will use the facility to make knockout mice important for analyzing molecules that are critical regulators of glutamatergic and GABAergic synapse development in the mammalian CNS. These include class 4 Semaphorins, which she identified in a largescale RNAi screen 1. Continued support of the facility would permit these investigators to obtain sufficient preliminary results to seek additional NINDS funding.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
Project #
Application #
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Brandeis University
United States
Zip Code
Matts, Jessica A; Sytnikova, Yuliya; Chirn, Gung-Wei et al. (2014) Small RNA library construction from minute biological samples. Methods Mol Biol 1093:123-36
Rosado, Michelle; Barber, Cynthia F; Berciu, Cristina et al. (2014) Critical roles for multiple formins during cardiac myofibril development and repair. Mol Biol Cell 25:811-27
Ghiretti, Amy E; Moore, Anna R; Brenner, Rebecca G et al. (2014) Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo. J Neurosci 34:392-407
Moore, Anna R; Ghiretti, Amy E; Paradis, Suzanne (2013) A loss-of-function analysis reveals that endogenous Rem2 promotes functional glutamatergic synapse formation and restricts dendritic complexity. PLoS One 8:e74751
Hall, Sarah E; Chirn, Gung-Wei; Lau, Nelson C et al. (2013) RNAi pathways contribute to developmental history-dependent phenotypic plasticity in C. elegans. RNA 19:306-19
Kuzirian, Marissa S; Moore, Anna R; Staudenmaier, Emily K et al. (2013) The class 4 semaphorin Sema4D promotes the rapid assembly of GABAergic synapses in rodent hippocampus. J Neurosci 33:8961-73
Perrat, Paola N; DasGupta, Shamik; Wang, Jie et al. (2013) Transposition-driven genomic heterogeneity in the Drosophila brain. Science 340:91-5
Ghiretti, Amy E; Kenny, Katelyn; Marr 2nd, Michael T et al. (2013) CaMKII-dependent phosphorylation of the GTPase Rem2 is required to restrict dendritic complexity. J Neurosci 33:6504-15
Luther, J A; Enes, J; Birren, S J (2013) Neurotrophins regulate cholinergic synaptic transmission in cultured rat sympathetic neurons through a p75-dependent mechanism. J Neurophysiol 109:485-96
Ghiretti, Amy E; Paradis, Suzanne (2011) The GTPase Rem2 regulates synapse development and dendritic morphology. Dev Neurobiol 71:374-89

Showing the most recent 10 out of 46 publications