This Neuroscience Center Core Facility, directed by Drs. Xandra Breakefield and Bakhos Tannous will be an institutionally unique resource which will bring our neuroscience faculty together and enhance their research on neurologic disorders through powerful, synergistic new technologies. The three new proposed cores are truly state-of-the-art. The Imaging Core (directed by Drs. Brad Hyman and Marian DiFiglia) will include array tomography, transmission electron microscopy and in vivo multiphoton imaging of detailed brain structure. The Microfluidics Core (directed by Drs. Daniel Irimia and Mehmet Toner) will provide specialized microfluidic devices and expertise in analysis for separation of cells and cell organelles, and high precision, real-time imaging of cell migration and axon guidance. The Vector Core (directed by Drs. Bakhos Tannous and Xandra Breakefield) will generate a variety of viral vectors for all projects and continue to expand its repertoire with advances in this field now including 10 serotypes of adeno-associated virus (AAV) vectors and coat-deficient rabies virus. The administration organization overseeing this program will include the P30 Director/Co-Director and Core Directors/Co-Directors to monitor ongoing activities and to interface with users; a Steering Committee to review operating procedures and activity reports on an annual basis, and to advise on prioritization issues and core effectiveness; an Advisory Board to offer advice on technology updates and management issues; and an Administrative Core which will logistically support the program with a dedicated senior grants manager All these services will be offered free-of-charge to the NINDS-funded neuroscience investigators at our institution and will serve to create a very dynamic, interactive environment where ideas become reality. This group of neuroscience investigators includes international leaders in the field, as well as junior and mid-level investigators for whom research on neurologic disorders is the main focus of their work and who have made many major contributions in the field. Our research covers a broad range of neurologic disorders due to stroke, injury, brain tumors and seizures, as well as neurodegenerative diseases including Alzheimer's disease, Parkinson's disease and Huntington's disease, and pediatric disorders, such as early onset dystonia, familial dysautonomia, hereditary sensory and autonomic neuropathy and adrenoleukodystrophy. Our vision for this PSO Core Facility is to achieve a deeper level of understanding of disease pathogenesis and to discover means of alleviation by providing technologies which can achieve high resolution of brain structure.

Public Health Relevance

Our program will support basic and translational research on a large number of neurologic disorders which have devastating on humans. The availability of the proposed high resolution technologies will be critical in elucidating the molecular and cellula etiologies of these disorders. In a hospital setting we are well positioned to translate our findins into improved diagnosis and therapy, and have a strong track record in improving patient care in neurologic disorders through research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045776-14
Application #
9391702
Study Section
Special Emphasis Panel (ZNS1)
Program Officer
Stewart, Randall R
Project Start
2003-05-01
Project End
2018-11-30
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
14
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02114
Teng, Jian; Carla da Hora, Cintia; Kantar, Rami S et al. (2017) Dissecting inherent intratumor heterogeneity in patient-derived glioblastoma culture models. Neuro Oncol 19:820-832
Mandelblat-Cerf, Yael; Kim, Angela; Burgess, Christian R et al. (2017) Bidirectional Anticipation of Future Osmotic Challenges by Vasopressin Neurons. Neuron 93:57-65
Merkel, Steven F; Andrews, Allison M; Lutton, Evan M et al. (2017) Trafficking of adeno-associated virus vectors across a model of the blood-brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells. J Neurochem 140:216-230
Wassmer, Sarah J; Carvalho, Livia S; György, Bence et al. (2017) Exosome-associated AAV2 vector mediates robust gene delivery into the murine retina upon intravitreal injection. Sci Rep 7:45329
Lagerweij, Tonny; Dusoswa, Sophie A; Negrean, Adrian et al. (2017) Optical clearing and fluorescence deep-tissue imaging for 3D quantitative analysis of the brain tumor microenvironment. Angiogenesis 20:533-546
Teng, Jian; Hejazi, Seyedali; Hiddingh, Lotte et al. (2017) Recycling drug screen repurposes hydroxyurea as a sensitizer of glioblastomas to temozolomide targeting de novo DNA synthesis, irrespective of molecular subtype. Neuro Oncol :
Maesako, Masato; Horlacher, Jana; Zoltowska, Katarzyna M et al. (2017) Pathogenic PS1 phosphorylation at Ser367. Elife 6:
Wong, Keith H K; Tessier, Shannon N; Miyamoto, David T et al. (2017) Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells. Nat Commun 8:1733
György, Bence; Sage, Cyrille; Indzhykulian, Artur A et al. (2017) Rescue of Hearing by Gene Delivery to Inner-Ear Hair Cells Using Exosome-Associated AAV. Mol Ther 25:379-391
Arbel-Ornath, Michal; Hudry, Eloise; Boivin, Josiah R et al. (2017) Soluble oligomeric amyloid-? induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain. Mol Neurodegener 12:27

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