The pluripotency of ES cells, combined with their ease of genetic manipulation and selection, has revolutionized gene functional studies in vivo via the generation of transgenic, chimeric and knockout mice. New technologies are evolving that will make the ability to perform appropriate manipulations of ES cells even more important for the next generation of mouse mutants (Draper and Nagy, 2007). These include, for example, the combination of inducible recombinases (Cre and Flp) for regulated temporal and spatial ablation of genes, RNAi, and development of new techniques that accelerate generation of completely ES cell derived FO mice (i.e. tetraploid aggregation and laser-assisted injection). The main service of Core 4 is to provide to NINDS-funded Qualifying Investigators a high level of expertise in mouse ES cell targeting in order to permit the efficient generation of animal models for neurobiological research. In addition to ES cell electroporation and characterization, the staff of the Core 4 will work with the NINDS-funded investigators to establish and characterize ES cell lines from a number of genetic backgrounds (particularly C57BI/6) that allow mice to be generated on a pure genetic background. Finally, Core 4 will maintain a bank of genetically marked ES cell lines. The ES cells and mice generated from these lines will be used for in vivo lineage tracing and in vitro differentiation into neuronal and glial cell lines.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045892-09
Application #
8374466
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
9
Fiscal Year
2012
Total Cost
$118,511
Indirect Cost
$38,436
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Sinnett, Sarah E; Hector, Ralph D; Gadalla, Kamal K E et al. (2017) Improved MECP2 Gene Therapy Extends the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Mol Ther Methods Clin Dev 5:106-115
McHenry, Jenna A; Otis, James M; Rossi, Mark A et al. (2017) Hormonal gain control of a medial preoptic area social reward circuit. Nat Neurosci 20:449-458
Decot, Heather K; Namboodiri, Vijay M K; Gao, Wei et al. (2017) Coordination of Brain-Wide Activity Dynamics by Dopaminergic Neurons. Neuropsychopharmacology 42:615-627
Hirsch, Matthew L; Conatser, Laura M; Smith, Sara M et al. (2017) AAV vector-meditated expression of HLA-G reduces injury-induced corneal vascularization, immune cell infiltration, and fibrosis. Sci Rep 7:17840
Dong, Enheng; Bachleda, Amelia; Xiong, Yubin et al. (2017) Reduced phosphoCREB in Müller glia during retinal degeneration in rd10 mice. Mol Vis 23:90-102
Otis, James M; Namboodiri, Vijay M K; Matan, Ana M et al. (2017) Prefrontal cortex output circuits guide reward seeking through divergent cue encoding. Nature 543:103-107
Bigler, Rebecca L; Kamande, Joyce W; Dumitru, Raluca et al. (2017) Messenger RNAs localized to distal projections of human stem cell derived neurons. Sci Rep 7:611
Guo, Jiami; Otis, James M; Higginbotham, Holden et al. (2017) Primary Cilia Signaling Shapes the Development of Interneuronal Connectivity. Dev Cell 42:286-300.e4
Irvin, David M; McNeill, Robert S; Bash, Ryan E et al. (2017) Intrinsic Astrocyte Heterogeneity Influences Tumor Growth in Glioma Mouse Models. Brain Pathol 27:36-50
McCoy, Eric S; Taylor-Blake, Bonnie; Aita, Megumi et al. (2017) Enhanced Nociception in Angelman Syndrome Model Mice. J Neurosci 37:10230-10239

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