Massively parallel assessment of the copy number and primary sequence of nucleic acids using sequence specific hybridization of tagged and affixed oligonucleotides has allowed for unprecedented analysis of gene expression and DMA sequence variation (Hoheisel, 2006). The amount and complexity of data that can be collected using this technology will place increasing technical demands on neuroscience investigators. To facilitate use of genomics tools by NINDS-funded investigators we are partnering with other members of the UNC genomics community. The Neuroscience Center Genomics and Bioinformatics Core has lead the UNC campus as an early adopter of genomics technology with the early acquisition of an Affymetrix GeneChip station with NINDS support. Subsequently, the Neuroscience Center, Carolina Center for Genome Sciences (CCGS), Lineberger Comprehensive Cancer Center (LCCC), and the UNC Department of Genetics have made complementary efforts to establish the infrastructure for additional genomic analysis. Dr. Kirk Wilhelmsen, a member of each of these Centers, both the Departments of Neurology and Genetics, as well as faculty advisor for both the CCGS genotyping and sequencing cores, has been instrumental in developing genomic infrastructure at UNC since his arrival three years ago. Dr. Chuck Perou has led the LCCC genomics efforts and developed bioinformatics infrastructure for transcriptional profiling (for example see the LCCC microarray databases at https://genome.unc.edu/ and https://dragon.med.unc.edu). During the last twelve months Dr. Wilhelmsen has organized and arranged funding for four equipment upgrades to the Core 1 Affymetrix platform including: an upgrade to allow scanning of the next generation of chips;acquisitions of a second fluidic workstation and an autoloader for the scanner to facilitate continuous data acquisition for large sets of chips;and a upgrade to so that that the platform can scan in four colors which is necessary for custom targeted genotyping panels (MIPS) and comparative genomic hybridization. His lab has determined more than 240 million genotypes for human neurodegenerative disease studies using Affymetrix 500K chips and 2.6 million genotypes using Affymetrix MIPS technology. These upgrades and expansion of the facilities were done while maintaining the ongoing support of transcriptional profiling (an average of 350 samples/per year) in support of NINDS-funded and other neuroscience investigators, and other NIH funded scientists at UNC that use the core. Dr Wilhelmsen's role as Core 1 director and Dr. Perou's role as consultant will facilitate integration of Core 1 with other genomics activity on campus to reduce redundancy and help maintain best practices.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045892-10
Application #
8485509
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-12-01
Budget End
2013-11-30
Support Year
10
Fiscal Year
2013
Total Cost
$90,031
Indirect Cost
$29,199
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Song, Liujiang; Llanga, Telmo; Conatser, Laura M et al. (2018) Serotype survey of AAV gene delivery via subconjunctival injection in mice. Gene Ther 25:402-414
Zhang, Jing; Wu, Tao; Simon, Jeremy et al. (2018) VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma. Science 361:290-295
Boyer, Nicholas P; Monkiewicz, Caroline; Menon, Shalini et al. (2018) Mammalian TRIM67 Functions in Brain Development and Behavior. eNeuro 5:
Sidorov, Michael S; Judson, Matthew C; Kim, Hyojin et al. (2018) Enhanced Operant Extinction and Prefrontal Excitability in a Mouse Model of Angelman Syndrome. J Neurosci 38:2671-2682
Crowther, Andrew J; Lim, Szu-Aun; Asrican, Brent et al. (2018) An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. Stem Cell Reports 10:1146-1159
Allard, Denise E; Wang, Yan; Li, Jian Joel et al. (2018) Schwann cell-derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment. J Clin Invest 128:4727-4741
Yeh, Chia-Yu; Asrican, Brent; Moss, Jonathan et al. (2018) Mossy Cells Control Adult Neural Stem Cell Quiescence and Maintenance through a Dynamic Balance between Direct and Indirect Pathways. Neuron 99:493-510.e4
Thaxton, Courtney; Kloth, Alexander D; Clark, Ellen P et al. (2018) Common Pathophysiology in Multiple Mouse Models of Pitt-Hopkins Syndrome. J Neurosci 38:918-936
Decot, Heather K; Namboodiri, Vijay M K; Gao, Wei et al. (2017) Coordination of Brain-Wide Activity Dynamics by Dopaminergic Neurons. Neuropsychopharmacology 42:615-627
McCoy, Eric S; Taylor-Blake, Bonnie; Aita, Megumi et al. (2017) Enhanced Nociception in Angelman Syndrome Model Mice. J Neurosci 37:10230-10239

Showing the most recent 10 out of 158 publications