The ability to rapidly identify and clone genes of interest, synthesize cRNA probes, and resolve regional and cellular expression has facilitated current understanding of cellular diversity in the brain, as well as appreciation of relationships between mutations, gene expression, and aberrant phenotypes. In parallel, quantitative PCR has emerged as a complimentary method to assess variation in levels as well as patterns of expression. These two methods provide a necessary transition from molecular discovery-based approaches such as expression microarray to hypothesis-driven experiments to understand gene function in the brain. The integration of expression analysis with other Core services described in this proposal facilitates the most difficult step in molecular and genetic analyses of CMS development or function: translation of molecular data into cell biological and systemic insight. The expansion of Core 2 services to include quantitative PCR as well as a well-developed library of validated probe templates will help insure the high level of productivity maintained by NINDS-supported investigators who accessed and used core services in the past 4 years.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS045892-10
Application #
8485510
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-12-01
Budget End
2013-11-30
Support Year
10
Fiscal Year
2013
Total Cost
$88,366
Indirect Cost
$28,660
Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Song, Liujiang; Llanga, Telmo; Conatser, Laura M et al. (2018) Serotype survey of AAV gene delivery via subconjunctival injection in mice. Gene Ther 25:402-414
Zhang, Jing; Wu, Tao; Simon, Jeremy et al. (2018) VHL substrate transcription factor ZHX2 as an oncogenic driver in clear cell renal cell carcinoma. Science 361:290-295
Boyer, Nicholas P; Monkiewicz, Caroline; Menon, Shalini et al. (2018) Mammalian TRIM67 Functions in Brain Development and Behavior. eNeuro 5:
Sidorov, Michael S; Judson, Matthew C; Kim, Hyojin et al. (2018) Enhanced Operant Extinction and Prefrontal Excitability in a Mouse Model of Angelman Syndrome. J Neurosci 38:2671-2682
Crowther, Andrew J; Lim, Szu-Aun; Asrican, Brent et al. (2018) An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus. Stem Cell Reports 10:1146-1159
Allard, Denise E; Wang, Yan; Li, Jian Joel et al. (2018) Schwann cell-derived periostin promotes autoimmune peripheral polyneuropathy via macrophage recruitment. J Clin Invest 128:4727-4741
Yeh, Chia-Yu; Asrican, Brent; Moss, Jonathan et al. (2018) Mossy Cells Control Adult Neural Stem Cell Quiescence and Maintenance through a Dynamic Balance between Direct and Indirect Pathways. Neuron 99:493-510.e4
Thaxton, Courtney; Kloth, Alexander D; Clark, Ellen P et al. (2018) Common Pathophysiology in Multiple Mouse Models of Pitt-Hopkins Syndrome. J Neurosci 38:918-936
Decot, Heather K; Namboodiri, Vijay M K; Gao, Wei et al. (2017) Coordination of Brain-Wide Activity Dynamics by Dopaminergic Neurons. Neuropsychopharmacology 42:615-627
McCoy, Eric S; Taylor-Blake, Bonnie; Aita, Megumi et al. (2017) Enhanced Nociception in Angelman Syndrome Model Mice. J Neurosci 37:10230-10239

Showing the most recent 10 out of 158 publications