This NINDS-supported Core (formerly called BAG Engineering Technology) has been in operation for nine years under the direction of Dr. JrGang ,Cheng, a pioneer in the field of BAC recombineering. Over this time, our Core established a local and national reputation for excellence in generating BAC transgenes and gene? targeting constructs (Essential function #1 below, Figs. 1.1 &1.2). To capitalize on the molecular expertise of the core and economies of scale, we propose to expand its essential functions (to include items #2-4 below) in this renewal period. ?

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
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Special Emphasis Panel (ZNS1-SRB-R (59))
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University of North Carolina Chapel Hill
Chapel Hill
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Burette, Alain C; Park, Haram; Weinberg, Richard J (2014) Postsynaptic distribution of IRSp53 in spiny excitatory and inhibitory neurons. J Comp Neurol 522:2164-78
Wright, Brittany D; Loo, Lipin; Street, Sarah E et al. (2014) The lipid kinase PIP5K1C regulates pain signaling and sensitization. Neuron 82:836-47
Huang, Hsien-Sung; Yoon, Bong-June; Brooks, Sherian et al. (2014) Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice. PLoS One 9:e98383
Mabb, Angela M; Kullmann, Paul H M; Twomey, Margaret A et al. (2014) Topoisomerase 1 inhibition reversibly impairs synaptic function. Proc Natl Acad Sci U S A 111:17290-5
Zhu, Hu; Pleil, Kristen E; Urban, Daniel J et al. (2014) Chemogenetic inactivation of ventral hippocampal glutamatergic neurons disrupts consolidation of contextual fear memory. Neuropsychopharmacology 39:1880-92
Demyanenko, Galina P; Mohan, Vishwa; Zhang, Xuying et al. (2014) Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling. J Neurosci 34:11274-87
Larsen, Rylan S; Smith, Ikuko T; Miriyala, Jayalakshmi et al. (2014) Synapse-specific control of experience-dependent plasticity by presynaptic NMDA receptors. Neuron 83:879-93
McNeill, Robert S; Schmid, Ralf S; Bash, Ryan E et al. (2014) Modeling astrocytoma pathogenesis in vitro and in vivo using cortical astrocytes or neural stem cells from conditional, genetically engineered mice. J Vis Exp :
Gama, Vivian; Swahari, Vijay; Schafer, Johanna et al. (2014) The E3 ligase PARC mediates the degradation of cytosolic cytochrome c to promote survival in neurons and cancer cells. Sci Signal 7:ra67
Morgan-Smith, Meghan; Wu, Yaohong; Zhu, Xiaoqin et al. (2014) GSK-3 signaling in developing cortical neurons is essential for radial migration and dendritic orientation. Elife 3:e02663

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