Rationale and Mission. The development of advanced microscopy techniques is a driving force behind advances in biological research and medical imaging. In the past several decades, the refinement and commercialization of confocal/multiphoton microscopes along with advances in fluorophore development (such as GFP derivatives, Chameleons and many others) have led to a renaissance in biological microscopy (Denk et al., Science 248, 1990;Piston, Trends Cell Biol 9, 1999;Diaspro, 2002, Wiley-Liss;Van Roessel and Brand, Nat Cell Biol 4, 2002;Giepmans et al., Science 312, 2006). The newest imaging methods, however, often require expertise and instruments beyond the financial and technical capacity of most individual scientists. Thus, shared imaging facilities have become crucial components of university research cores. The CNR Imaging &Cell Analysis Core was developed in response to the rapid advances occurring in contemporary biological microscopy and to meet the imaging priorities of Tufts neuroscience investigators. The core now provides Tufts neuroscientists with a broad range of microscopic imaging services including widefield fluorescence microscopy &digital imaging, confocal microscopy, 2-photon microscopy, live-cell imaging, electron microscopy, and laser capture microdissection. In addition to standard microscopic investigations, investigators use core facilities to perform dynamic imaging studies such as fluorescence resonance energy transfer (FRET) or fluorescence recovery after photobleaching (FRAP) experiments. Core equipment and services are described in a later section.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS047243-09
Application #
8374492
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
9
Fiscal Year
2012
Total Cost
$170,050
Indirect Cost
$66,990
Name
Tufts University
Department
Type
DUNS #
039318308
City
Boston
State
MA
Country
United States
Zip Code
02111
Mukherjee, Jayanta; Cardarelli, Ross A; Cantaut-Belarif, Yasmine et al. (2017) Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses. Proc Natl Acad Sci U S A 114:11763-11768
Khayat, A; Monteiro, N; Smith, E E et al. (2017) GelMA-Encapsulated hDPSCs and HUVECs for Dental Pulp Regeneration. J Dent Res 96:192-199
Felsted, Jennifer A; Chien, Cheng-Hao; Wang, Dongqing et al. (2017) Alpha2delta-1 in SF1+ Neurons of the Ventromedial Hypothalamus Is an Essential Regulator of Glucose and Lipid Homeostasis. Cell Rep 21:2737-2747
Salzberg, Yehuda; Coleman, Andrew J; Celestrin, Kevin et al. (2017) Reduced Insulin/Insulin-Like Growth Factor Receptor Signaling Mitigates Defective Dendrite Morphogenesis in Mutants of the ER Stress Sensor IRE-1. PLoS Genet 13:e1006579
Urra, Félix A; Muñoz, Felipe; Lovy, Alenka et al. (2017) The Mitochondrial Complex(I)ty of Cancer. Front Oncol 7:118
Morel, Lydie; Chiang, Ming Sum R; Higashimori, Haruki et al. (2017) Molecular and Functional Properties of Regional Astrocytes in the Adult Brain. J Neurosci 37:8706-8717
Papouin, Thomas; Dunphy, Jaclyn M; Tolman, Michaela et al. (2017) Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness. Neuron 94:840-854.e7
Bustos, Galdo; Cruz, Pablo; Lovy, Alenka et al. (2017) Endoplasmic Reticulum-Mitochondria Calcium Communication and the Regulation of Mitochondrial Metabolism in Cancer: A Novel Potential Target. Front Oncol 7:199
Hawley, Dillon; Ding, Jian; Thotakura, Suharika et al. (2017) RNA-Seq and CyTOF immuno-profiling of regenerating lacrimal glands identifies a novel subset of cells expressing muscle-related proteins. PLoS One 12:e0179385
Herwald, Sanna E; Zucchi, Paola C; Tan, Shumin et al. (2017) The two transmembrane regions of Candida albicans Dfi1 contribute to its biogenesis. Biochem Biophys Res Commun 488:153-158

Showing the most recent 10 out of 200 publications