Zebrafish Core C3.1 Rationale. Core C maintains wild type, transgenic and mutant zebrafish strains for RMNDC investigators. The unique developmental and optical imaging properties of the zebrafish embryo makes it an ideal model to address questions at the heart of the research programs RMNDC investigators. UCD NINDS researchers are internationally recognized and expert in the fields of neurophysiology, neurodevelopment and optical imaging. During the previous award period. Core C removed technical barriers for RMNDC investigators who were interested in using the model system (e.g., Betz, Caldwell, Clouthier, Niswander, Restrepo, Taylor, Williams) but did not have experience or means for maintaining lines. We propose three Specific Aims for Core C that will allow RMNDC members to take advantage of the zebrafish system for neurodevelopmental and behavioral studies. In addition to the 13 current Core C users, the facility will allow access to the zebrafish model to investigators who have not considered its use because of the pragmatic limitations associated with maintaining and breeding zebrafish. In particular, there has been growing interest in complementing rodent studies (e.g.. Core B) with ones done in zebrafish (e.g., Appel, Artinger, Barlow, Clouthier, Macklin, Niswander, Restrepo, Williams), and vice versa. The core will also be a resource for the larger scientific community because transgenic lines will be described on the RMNDC website and made available to the Zebrafish International Resource Center (ZIRC) by the time of the first publication.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS048154-09
Application #
8585910
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
9
Fiscal Year
2014
Total Cost
$108,012
Indirect Cost
$37,415
Name
University of Colorado Denver
Department
Type
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Sinnen, Brooke L; Bowen, Aaron B; Forte, Jeffrey S et al. (2017) Optogenetic Control of Synaptic Composition and Function. Neuron 93:646-660.e5
Soltys, John N; Meyer, Stephanie A; Schumann, Hannah et al. (2017) Determining the Spatial Relationship of Membrane-Bound Aquaporin-4 Autoantibodies by STED Nanoscopy. Biophys J 112:1692-1702
Goodell, Dayton J; Zaegel, Vincent; Coultrap, Steven J et al. (2017) DAPK1 Mediates LTD by Making CaMKII/GluN2B Binding LTP Specific. Cell Rep 19:2231-2243
Moreno, Rosa L; Josey, Megan; Ribera, Angeles B (2017) Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons. J Vis Exp :
Dittmer, Philip J; Wild, Angela R; Dell'Acqua, Mark L et al. (2017) STIM1 Ca2+ Sensor Control of L-type Ca2+-Channel-Dependent Dendritic Spine Structural Plasticity and Nuclear Signaling. Cell Rep 19:321-334
Gilmer, Jesse I; Person, Abigail L (2017) Morphological Constraints on Cerebellar Granule Cell Combinatorial Diversity. J Neurosci 37:12153-12166
Westacott, Matthew J; Ludin, Nurin W F; Benninger, Richard K P (2017) Spatially Organized ?-Cell Subpopulations Control Electrical Dynamics across Islets of Langerhans. Biophys J 113:1093-1108
Lam, Philip M; Carlsen, Jessica; González, Marco I (2017) A calpain inhibitor ameliorates seizure burden in an experimental model of temporal lobe epilepsy. Neurobiol Dis 102:1-10
McElroy, Pallavi B; Liang, Li-Ping; Day, Brian J et al. (2017) Scavenging reactive oxygen species inhibits status epilepticus-induced neuroinflammation. Exp Neurol 298:13-22
Westacott, Matthew J; Farnsworth, Nikki L; St Clair, Joshua R et al. (2017) Age-Dependent Decline in the Coordinated [Ca2+] and Insulin Secretory Dynamics in Human Pancreatic Islets. Diabetes 66:2436-2445

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