The Microscopy, Image Analysis and Stereology Core, located on the BBSRB fourth floor, provides advanced instrumentation required for neurotrauma and other neuroscience research, including two Olympus Provis AX70 Fluorescence Microscopes (Image Pro interfaced via MagnaFire Digital Camera), an Olympus BX 41 with Video Camera, an Olympus BX 50 Epifluorescent Microscope (Bioquant Stereology package + 3D topographer interfaced with MagnaFire Digital Camera and Optronics Video Camera), a Zeiss Axiovert 200 M microscope, and a Nikon E400 teaching microscope with DAGE Video Camera. In addition to this existing equipment, SCoBIRC recently invested in three new specialized microscopes with funding from the NIH. The first, an Olympus FluoViewTM 300 point-scanning, point-detection, confocal laser scanning microscope designed for biology research applications, has excellent resolution, efficiency of excitation, and an intuitive, user-friendly interface including preset parameters for individual users. The FluoViewTM 300 is also capable of 3D rendering and time-lapse observation, and TTL I/O signals can be generated to coordinate experiments timed with external instrumentation. Additionally, two high-sensitivity photomultiplier tubes (PMTs) are located directly within the FV300 confocal fluorescence emission light path for high sensitivity detection of the fluorescence signal. The second stereology microscope is an inverted Olympus IX 81 outfitted with motorized stage encoders and the BioQuant Nova Prime software, particulariy well suited for quantification using modern stereological tools. The core is staffed by a Core Director, Patrick Sullivan, Ph.D., SCoBIRC Associate Director and Associate Professor of Anatomy &Neurobiology and an Assistant Director, Alexander Rabchevsky, Ph.D., Associate Professor of Physiology both of whom are well versed in the use of multiple image analysis endpoint measures and confocal imaging as endpoints in theirresearch. Both have expertise in developing protocols used to assess tissue sparing and cell loss in neurotrauma research.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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University of Kentucky
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Yonutas, Heather M; Pandya, Jignesh D; Sullivan, Patrick G (2015) Changes in mitochondrial bioenergetics in the brain versus spinal cord become more apparent with age. J Bioenerg Biomembr 47:149-54
Carlson, Shaun W; Madathil, Sindhu K; Sama, Diana M et al. (2014) Conditional overexpression of insulin-like growth factor-1 enhances hippocampal neurogenesis and restores immature neuron dendritic processes after traumatic brain injury. J Neuropathol Exp Neurol 73:734-46
Bolton, Amanda N; Saatman, Kathryn E (2014) Regional neurodegeneration and gliosis are amplified by mild traumatic brain injury repeated at 24-hour intervals. J Neuropathol Exp Neurol 73:933-47
Pandya, Jignesh D; Readnower, Ryan D; Patel, Samir P et al. (2014) N-acetylcysteine amide confers neuroprotection, improves bioenergetics and behavioral outcome following TBI. Exp Neurol 257:106-13
Miller, Darren M; Wang, Juan A; Buchanan, Ashley K et al. (2014) Temporal and spatial dynamics of nrf2-antioxidant response elements mediated gene targets in cortex and hippocampus after controlled cortical impact traumatic brain injury in mice. J Neurotrauma 31:1194-201
Srodulski, Sarah; Sharma, Savita; Bachstetter, Adam B et al. (2014) Neuroinflammation and neurologic deficits in diabetes linked to brain accumulation of amylin. Mol Neurodegener 9:30
Singh, Ranjana; Brewer, M Kathryn; Mashburn, Charles B et al. (2014) Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies. J Biol Chem 289:19383-94
Yu, C G; Singh, R; Crowdus, C et al. (2014) Fenbendazole improves pathological and functional recovery following traumatic spinal cord injury. Neuroscience 256:163-9
Patel, Samir P; Sullivan, Patrick G; Pandya, Jignesh D et al. (2014) N-acetylcysteine amide preserves mitochondrial bioenergetics and improves functional recovery following spinal trauma. Exp Neurol 257:95-105
Miller, Darren M; Singh, Indrapal N; Wang, Juan A et al. (2013) Administration of the Nrf2-ARE activators sulforaphane and carnosic acid attenuates 4-hydroxy-2-nonenal-induced mitochondrial dysfunction ex vivo. Free Radic Biol Med 57:1-9

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