The major benefits from the core are: 1) creation of a repository for all the expression plasmids needed to tag proteins for pull down-based protein isolation procedures and for validating putative binding interactions;2) provision of shuttle vectors needed to produce lentivirus;3) provision of state of the art, well characterized lentivirus vectors to qualified investigators. Lentivirus vectors have proved exceptionally useful in providing efficient transduction of quiescent brain cells that have been traditionally resistant to retroviral-mediated transduction. Recombinant lentiviruses do not encode viral proteins and have the benefit of low toxicity and immunogenicity and produce stable proviral integrants. 4) provision of protocols for isolating tagged proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS055088-04
Application #
8374483
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
4
Fiscal Year
2012
Total Cost
$298,874
Indirect Cost
$107,289
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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