Purpose of the Core: The Genetic Manipulation Core provides Center investigators with the ability to manipulate gene expression in vitro and in vivo. This Core incorporates a twopronged strategy so that investigators can select the optimal method to meet their needs. The AAV Division provides recombinant viruses for manipulating target expression for instances where acute manipulation is sufficient. For those investigators requiring the ability to manipulate gene expression chronically in vivo, the Core provides bacterial artificial chromosome (BAC) transgene construction. An added advantage of this Core design is that it provides an investigator with the ability to first "test" a particular manipulation using the less expensive viral vector approach and then move to a BACbased approach as appropriate. For example, the Orr group is actively trying to identify the kinases and phosphatases that regulate the phosphorylation of ataxin-1 at Ser776, since this posttranslational modification is thought to regulate the normal function of the protein as well as SCA1 pathogenesis by mutant ataxin-1. Having the ability to modify the activity of enzymes in vivo Figure 2. Floorplans showing Core facilities for the Center (areas enclosed by red boxes), using recombinant viral vectors provides a very useful screen of candidates from cellular and biochemical studies before moving into transgenic mouse studies using a BAC-based approach.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
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National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
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University of Minnesota Twin Cities
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Wydeven, Nicole; Marron Fernandez de Velasco, Ezequiel; Du, Yu et al. (2014) Mechanisms underlying the activation of G-protein-gated inwardly rectifying K+ (GIRK) channels by the novel anxiolytic drug, ML297. Proc Natl Acad Sci U S A 111:10755-60
Armbrust, Karen R; Wang, Xinming; Hathorn, Tyisha J et al. (2014) Mutant ?-III spectrin causes mGluR1? mislocalization and functional deficits in a mouse model of spinocerebellar ataxia type 5. J Neurosci 34:9891-904
Moser, H R; Giesler Jr, G J (2014) Itch elicited by intradermal injection of serotonin, intracisternal injection of morphine, and their synergistic interactions in rats. Neuroscience 274:119-27
Martinez, Luis A; Peterson, Brittni M; Meisel, Robert L et al. (2014) Estradiol facilitation of cocaine-induced locomotor sensitization in female rats requires activation of mGluR5. Behav Brain Res 271:39-42
Le Naour, Morgan; Lunzer, Mary M; Powers, Michael D et al. (2014) Putative kappa opioid heteromers as targets for developing analgesics free of adverse effects. J Med Chem 57:6383-92
Moser, Hannah R; Giesler Jr, Glenn J (2014) Characterization of pruriceptive trigeminothalamic tract neurons in rats. J Neurophysiol 111:1574-89
Smith, Laura N; Jedynak, Jakub P; Fontenot, Miles R et al. (2014) Fragile X mental retardation protein regulates synaptic and behavioral plasticity to repeated cocaine administration. Neuron 82:645-58
Meitzen, John; Perry, Adam N; Westenbroek, Christel et al. (2013) Enhanced striatal *1-adrenergic receptor expression following hormone loss in adulthood is programmed by both early sexual differentiation and puberty: a study of humans and rats. Endocrinology 154:1820-31
Meitzen, John; Luoma, Jessie I; Boulware, Marissa I et al. (2013) Palmitoylation of estrogen receptors is essential for neuronal membrane signaling. Endocrinology 154:4293-304
Ebner, Blake A; Ingram, Melissa A; Barnes, Justin A et al. (2013) Purkinje cell ataxin-1 modulates climbing fiber synaptic input in developing and adult mouse cerebellum. J Neurosci 33:5806-20

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