Purpose of the Core: The Genetic Manipulation Core provides Center investigators with the ability to manipulate gene expression in vitro and in vivo. This Core incorporates a twopronged strategy so that investigators can select the optimal method to meet their needs. The AAV Division provides recombinant viruses for manipulating target expression for instances where acute manipulation is sufficient. For those investigators requiring the ability to manipulate gene expression chronically in vivo, the Core provides bacterial artificial chromosome (BAC) transgene construction. An added advantage of this Core design is that it provides an investigator with the ability to first """"""""test"""""""" a particular manipulation using the less expensive viral vector approach and then move to a BACbased approach as appropriate. For example, the Orr group is actively trying to identify the kinases and phosphatases that regulate the phosphorylation of ataxin-1 at Ser776, since this posttranslational modification is thought to regulate the normal function of the protein as well as SCA1 pathogenesis by mutant ataxin-1. Having the ability to modify the activity of enzymes in vivo Figure 2. Floorplans showing Core facilities for the Center (areas enclosed by red boxes), using recombinant viral vectors provides a very useful screen of candidates from cellular and biochemical studies before moving into transgenic mouse studies using a BAC-based approach.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS062158-04
Application #
8588812
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
4
Fiscal Year
2014
Total Cost
$146,662
Indirect Cost
$49,535
Name
University of Minnesota Twin Cities
Department
Type
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Victoria, Nicole C; Marron Fernandez de Velasco, Ezequiel; Ostrovskaya, Olga et al. (2016) G Protein-Gated K+ Channel Ablation in Forebrain Pyramidal Neurons Selectively Impairs Fear Learning. Biol Psychiatry 80:796-806
Lipshetz, Brett; Giesler Jr, Glenn J (2016) Effects of scratching and other counterstimuli on responses of trigeminothalamic tract neurons to itch-inducing stimuli in rats. J Neurophysiol 115:520-9
McBrayer, Zofeyah L; Dimova, Jiva; Pisansky, Marc T et al. (2015) Forebrain-Specific Loss of BMPRII in Mice Reduces Anxiety and Increases Object Exploration. PLoS One 10:e0139860
Cramer, Samuel W; Popa, Laurentiu S; Carter, Russell E et al. (2015) Abnormal excitability and episodic low-frequency oscillations in the cerebral cortex of the tottering mouse. J Neurosci 35:5664-79
Prosise, Jodi F; Hendrix, Claudia M; Ebner, Timothy J (2015) Joint angles and angular velocities and relevance of eigenvectors during prehension in the monkey. Exp Brain Res 233:339-50
Öz, Gülin; Kittelson, Emily; Demirgöz, Döne et al. (2015) Assessing recovery from neurodegeneration in spinocerebellar ataxia 1: Comparison of in vivo magnetic resonance spectroscopy with motor testing, gene expression and histology. Neurobiol Dis 74:158-66
Jansen, Nico A; Giesler Jr, Glenn J (2015) Response characteristics of pruriceptive and nociceptive trigeminoparabrachial tract neurons in the rat. J Neurophysiol 113:58-70
Moser, Hannah R; Giesler Jr, Glenn J (2014) Characterization of pruriceptive trigeminothalamic tract neurons in rats. J Neurophysiol 111:1574-89
Le Naour, Morgan; Lunzer, Mary M; Powers, Michael D et al. (2014) Putative kappa opioid heteromers as targets for developing analgesics free of adverse effects. J Med Chem 57:6383-92
Martinez, Luis A; Peterson, Brittni M; Meisel, Robert L et al. (2014) Estradiol facilitation of cocaine-induced locomotor sensitization in female rats requires activation of mGluR5. Behav Brain Res 271:39-42

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