In recent years there has been an increase in the generation of transgenic and knockout mouse lines as well as viral delivery of genes. These new genetic manipulations have revolutionized our understanding of the biological function of cellular and molecular processes in both normal and disease state of central nervous system ^^?^^. The large numberof mouse models calls for rigorous, sensitive, and reproducible investigations of behavioral phenotypes of mutant mice lines ^^ ^?, as well as pharmacological efficacy studies. Behavioral testing paradigms should be easily transferable and replicable between laboratories. Many of the standard behavioral tests used today are not easily reproduced between laboratories, due either to variation in the genetic background of mice ^^'^?, or to variations in environmental or experimental conditions ^^'^^. Other confounding factors can include variations in animal handling, housing, transportation, and test conditions ^^?^^? ^. Behavioral models used for phenotyping of mice are designed based on the models that have been designed for rats as the test subjects ^^. Often these classical tests do not satisfactorily transfer over to mice. Additionally, these standard tests introduce many outside variables such as the effects of environment and human handling ^ forced isolation, interruption of sleep/wake cycle, and placement in unnatural, stressful environments. In order to minimize these experimental and environmental variables, we will establish an Automated Behavioral Core to monitor and record mouse behavior in the home cage environment. These novel tests have the benefit of decreasing variability and increasing reproducibility of behavioral outcome between experimental repeats ^^"^?. By using these new technologies we are aiming to 1) reduce novelty factors inherent in many classical tests by performing the behavioral testing inside the home cage ^^;2) test the Individual animals while they remain in social groups (Intellicage) or when they are individually housed (PhenoLab);3) reduce the stress related to the handling and experimental condition;4) perform the data acquisition using computer software in an independent and unbiased manner;and 5) conduct the experiments with higher throughput during the animals active cycle without human interruption.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS069375-03
Application #
8427318
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2012-12-01
Budget End
2013-11-30
Support Year
3
Fiscal Year
2013
Total Cost
$320,085
Indirect Cost
$127,808
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Bennett, Mariko L; Bennett, F Chris; Liddelow, Shane A et al. (2016) New tools for studying microglia in the mouse and human CNS. Proc Natl Acad Sci U S A 113:E1738-46
Kuipers, Hedwich F; Rieck, Mary; Gurevich, Irina et al. (2016) Hyaluronan synthesis is necessary for autoreactive T-cell trafficking, activation, and Th1 polarization. Proc Natl Acad Sci U S A 113:1339-44
Bouyer, Charlène; Chen, Pu; Güven, Sinan et al. (2016) A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors. Adv Mater 28:161-7
Loh, Kyle M; Chen, Angela; Koh, Pang Wei et al. (2016) Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types. Cell 166:451-67
Gaudenzio, Nicolas; Sibilano, Riccardo; Marichal, Thomas et al. (2016) Different activation signals induce distinct mast cell degranulation strategies. J Clin Invest 126:3981-3998
Franco, Magdalena; Panas, Michael W; Marino, Nicole D et al. (2016) A Novel Secreted Protein, MYR1, Is Central to Toxoplasma's Manipulation of Host Cells. MBio 7:e02231-15
Woodling, Nathaniel S; Colas, Damien; Wang, Qian et al. (2016) Cyclooxygenase inhibition targets neurons to prevent early behavioural decline in Alzheimer's disease model mice. Brain 139:2063-81
Winans, Amy M; Collins, Sean R; Meyer, Tobias (2016) Waves of actin and microtubule polymerization drive microtubule-based transport and neurite growth before single axon formation. Elife 5:e12387
Chung, Won-Suk; Verghese, Philip B; Chakraborty, Chandrani et al. (2016) Novel allele-dependent role for APOE in controlling the rate of synapse pruning by astrocytes. Proc Natl Acad Sci U S A 113:10186-91
Lund, Peder J; Elias, Joshua E; Davis, Mark M (2016) Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol 197:3086-3098

Showing the most recent 10 out of 64 publications