(NMS) As one of three Stanford Neuroscience Cores, the Neuroscience Microscopy Service (NMS) provides capital-intensive microscopy equipment which is often beyond the budgets of individual labs, and expert training on the available microscopes and image analysis software to enable researchers to achieve the highest quality results. Fluorescence microscopy is an essential technique in current neuroscience research and, with the continual development of genetically encoded fluorescent reporters and new microscope technologies, will remain so for the foreseeable future. During each of the previous four years, NMS has served between 50 and 70 Pis with an annual average of 3500 hours of microscope usage and training time. The majority of this support has gone to NIH-funded neuroscience labs. Over the last four years, NMS has kept its microscopy services updated with the addition of a new SIM/STORM super-resolution microscope, a new laser scanning confocal microscope, the addition of electrophysiological recording capability to its two photon tissue slice rig, and the addition of a new high-powered image analysis workstation equipped with Neurolucida neuron tracing software. Under the proposed grant, NMS will continue to provide the most up-to-date and cutting edge services, including efforts to expand the use of its super-resolution microscope and two photon microscopes.

Public Health Relevance

Fluorescence microscopy is an essential technique in current neuroscience research and, with the continual development of genetically encoded fluorescent reporters and new microscope technologies, will remain so for the foreseeable future. As one of three Stanford Neuroscience Cores, the Neuroscience Microscopy Service (NMS) was created to provide capital-intensive fluorescence microscopy equipment which is often beyond the budgets of individual labs, and expert training on its use to enable researchers to achieve the highest quality results.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS069375-08
Application #
9380978
Study Section
Special Emphasis Panel (ZNS1)
Project Start
2011-03-01
Project End
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
8
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94304
Kornfeld, Opher S; Qvit, Nir; Haileselassie, Bereketeab et al. (2018) Interaction of mitochondrial fission factor with dynamin related protein 1 governs physiological mitochondrial function in vivo. Sci Rep 8:14034
Zhang, Zhenjie; Marro, Samuele G; Zhang, Yingsha et al. (2018) The fragile X mutation impairs homeostatic plasticity in human neurons by blocking synaptic retinoic acid signaling. Sci Transl Med 10:
Joshi, Amit U; Saw, Nay L; Shamloo, Mehrdad et al. (2018) Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget 9:6128-6143
Stamatakis, Alice M; Schachter, Mike J; Gulati, Srishti et al. (2018) Simultaneous Optogenetics and Cellular Resolution Calcium Imaging During Active Behavior Using a Miniaturized Microscope. Front Neurosci 12:496
Kramer, Nicholas J; Haney, Michael S; Morgens, David W et al. (2018) CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. Nat Genet 50:603-612
Li, Shi-Bin; Nevárez, Natalie; Giardino, William J et al. (2018) Optical probing of orexin/hypocretin receptor antagonists. Sleep 41:
Liu, Qing; Van Bortle, Kevin; Zhang, Yue et al. (2018) Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960
Razavi, Mehdi; Thakor, Avnesh S (2018) An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy. J Mater Sci Mater Med 29:54
Giardino, William J; Eban-Rothschild, Ada; Christoffel, Daniel J et al. (2018) Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci 21:1084-1095
Bennett, F Chris; Bennett, Mariko L; Yaqoob, Fazeela et al. (2018) A Combination of Ontogeny and CNS Environment Establishes Microglial Identity. Neuron 98:1170-1183.e8

Showing the most recent 10 out of 102 publications