The major goals of this core were to establish a core facility for the high content analysis of neurons and neural networks.
The aim was started with high content cellular imaging systems from BD biosciences to capture fluorescence images of cells in 96 and 384 well format as necessary for morphology based shRNA and drug screens. Since then we have significantly expanded the imaging resources with the addition of an Olympus VS120 slide scanning microscope, a Leica laser capture microdissection system, an Olympus Viva View environmental time lapse imaging microscope, and a home built selective plane imaging microscope (SPIM). With these technologies the core has the capabilities to analyze cells and circuits at a wide range of spatiotemporal resolutions in order to reveal their anatomical organization as well as the relationship between cellular content and function. The major goals have not changed but the capabilities have been expanded via the acquisition of the components described above.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30NS072030-04
Application #
8733768
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Harvard Medical School
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02115
Wang, Shan Shan H; Held, Richard G; Wong, Man Yan et al. (2016) Fusion Competent Synaptic Vesicles Persist upon Active Zone Disruption and Loss of Vesicle Docking. Neuron 91:777-91
Tang, Jonathan Cy; Drokhlyansky, Eugene; Etemad, Behzad et al. (2016) Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. Elife 5:
Jackman, Skyler L; Turecek, Josef; Belinsky, Justine E et al. (2016) The calcium sensor synaptotagmin 7 is required for synaptic facilitation. Nature 529:88-91
Granger, Adam J; Mulder, Nicole; Saunders, Arpiar et al. (2016) Cotransmission of acetylcholine and GABA. Neuropharmacology 100:40-6
Hu, Yu; Kim, Ji Hyung; He, Kangmin et al. (2016) Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion. J Exp Med 213:2759-2772
Mierau, Susanna B; Patrizi, Annarita; Hensch, Takao K et al. (2016) Cell-Specific Regulation of N-Methyl-D-Aspartate Receptor Maturation by Mecp2 in Cortical Circuits. Biol Psychiatry 79:746-54
Held, Richard G; Liu, Changliang; Kaeser, Pascal S (2016) ELKS controls the pool of readily releasable vesicles at excitatory synapses through its N-terminal coiled-coil domains. Elife 5:
Witter, Laurens; Rudolph, Stephanie; Pressler, R Todd et al. (2016) Purkinje Cell Collaterals Enable Output Signals from the Cerebellar Cortex to Feed Back to Purkinje Cells and Interneurons. Neuron 91:312-9
Saunders, Arpiar; Huang, Kee Wui; Sabatini, Bernardo Luis (2016) Globus Pallidus Externus Neurons Expressing parvalbumin Interconnect the Subthalamic Nucleus and Striatal Interneurons. PLoS One 11:e0149798
Wang, Chih-Chieh; Weyrer, Christopher; Paturu, Mounica et al. (2016) Calcium-Dependent Protein Kinase C Is Not Required for Post-Tetanic Potentiation at the Hippocampal CA3 to CA1 Synapse. J Neurosci 36:6393-402

Showing the most recent 10 out of 37 publications