Individual NINDS grantees have strived to employ and develop cutting-edge genetic tools to manipulate the mouse genome to test specific hypotheses proposed in their NINDS-funded research projects. Producing many of these lines has required multiple steps in generating mouse ES cells and these lines are often laborious or difficult to obtain. The cost and time for generating such ES lines is a major bottleneck for successful animal model development as it prohibits many NINDS funded investigators from generating genetically-engineering mice, even for labs that are versed in the required advanced and very specific technologies. A central genome modification core will provide services to break the barriers to generate genetically modified mouse lines by supporting the production of designer ES-cell lines. A centralized GM Core will increase efficiency and lower the cost of generating such lines. In addition, a central service will provide opportunities for synergistic development of lines that will be of value across all user groups. The Institute has made a commitment to provide space for establishment of the GM core that would lead the way to meet the evolving needs of Neuroscience Center investigators. The major goal of the GM Core is to provide services for manipulation ofthe mouse genome in ES cells that are essential for studies ofthe mechanisms of neural function, structure and development as well as the generation of both in vitro and in vivo mouse models of diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS072031-04
Application #
8666073
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
4
Fiscal Year
2014
Total Cost
$67,363
Indirect Cost
$32,002
Name
Salk Institute for Biological Studies
Department
Type
DUNS #
078731668
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Hilde, Kathryn L; Levine, Ariel J; Hinckley, Christopher A et al. (2016) Satb2 Is Required for the Development of a Spinal Exteroceptive Microcircuit that Modulates Limb Position. Neuron 91:763-76
Kawaguchi, Daichi; Sahara, Setsuko; Zembrzycki, Andreas et al. (2016) Generation and analysis of an improved Foxg1-IRES-Cre driver mouse line. Dev Biol 412:139-47
Xu, Jiqing; de Winter, Fred; Farrokhi, Catherine et al. (2016) Neuregulin 1 improves cognitive deficits and neuropathology in an Alzheimer's disease model. Sci Rep 6:31692
Kador, Karl E; Grogan, Shawn P; Dorthé, Erik W et al. (2016) Control of Retinal Ganglion Cell Positioning and Neurite Growth: Combining 3D Printing with Radial Electrospun Scaffolds. Tissue Eng Part A 22:286-94
Sekiguchi, Kohei J; Shekhtmeyster, Pavel; Merten, Katharina et al. (2016) Imaging large-scale cellular activity in spinal cord of freely behaving mice. Nat Commun 7:11450
Perez-Garcia, Carlos G; O'Leary, Dennis D M (2016) Formation of the Cortical Subventricular Zone Requires MDGA1-Mediated Aggregation of Basal Progenitors. Cell Rep 14:560-71
Kerman, Bilal E; Kim, Hyung Joon; Padmanabhan, Krishnan et al. (2015) In vitro myelin formation using embryonic stem cells. Development 142:2213-25
Taylor-Weiner, Hermes; Ravi, Neeraja; Engler, Adam J (2015) Traction forces mediated by integrin signaling are necessary for definitive endoderm specification. J Cell Sci 128:1961-8
Wassenaar, Jean W; Boss, Gerry R; Christman, Karen L (2015) Decellularized skeletal muscle as an in vitro model for studying drug-extracellular matrix interactions. Biomaterials 64:108-14
Adams, Michael W; Loftus, Andrew F; Dunn, Sarah E et al. (2015) Light Sheet Fluorescence Microscopy (LSFM). Curr Protoc Cytom 71:12.37.1-15

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